Strigolactones and Dysplastic Colonic Lesions or Cancer
- Conditions
- Colonic NeoplasmsColonic Polyps
- Interventions
- Procedure: biopsy during colonoscopy
- Registration Number
- NCT02824835
- Lead Sponsor
- Meir Medical Center
- Brief Summary
The purpose of this study is to determine the effects of the plant hormones strigolactones, on cell cultures of colonic polyps and colorectal cancer.
- Detailed Description
Strigolactones have been shown to anti-proliferative and pro-apoptotic effects in cell cultures of breast and prostate origin, partially by down-regulation of cyclin B1 and induction of cell cycle arrest. In this study the investigators intend to take biopsies from benign colonic polyps and cancer tissue, culture intestinal explants and generate immortalized primary cells as a platform to assess the effect of this plant hormone on cell viability, cell mobility, apoptosis and cytoskeleton modifications.
Recruitment & Eligibility
- Status
- UNKNOWN
- Sex
- All
- Target Recruitment
- 150
Inclusion Criteria
- Patients with positive findings during colonoscopy
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Exclusion Criteria
- Patients below 18 years old
- Patients who cannot sign a consent form
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Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description cancer group biopsy during colonoscopy Patients with colorectal cancer. Biopsies are taken before surgical resection. adenoma group biopsy during colonoscopy Patients with adenoma. Biopsies are taken before endoscopic resection.
- Primary Outcome Measures
Name Time Method percentage of cells with reduced viability after dose-dependent exposure 24 hours of exposure to strigolactone percentage of cells in G2/M arrest after dose-dependent exposure 24 hours of exposure to strigolactone analysis of cell cycle
- Secondary Outcome Measures
Name Time Method