The Effect of the Plant Hormones Strigolactones on Cultures of Dysplastic Colonic Lesions and Cancer
Trial Snapshot
- Phase
- Not Applicable
- Sponsor
- Meir Medical Center
- Enrollment
- 150
- Primary Endpoint
- percentage of cells in G2/M arrest after dose-dependent exposure
Study Overview
Brief Summary
The purpose of this study is to determine the effects of the plant hormones strigolactones, on cell cultures of colonic polyps and colorectal cancer.
Detailed Description
Strigolactones have been shown to anti-proliferative and pro-apoptotic effects in cell cultures of breast and prostate origin, partially by down-regulation of cyclin B1 and induction of cell cycle arrest. In this study the investigators intend to take biopsies from benign colonic polyps and cancer tissue, culture intestinal explants and generate immortalized primary cells as a platform to assess the effect of this plant hormone on cell viability, cell mobility, apoptosis and cytoskeleton modifications.
Study Design
- Study Type
- Observational
- Observational Model
- Case Only
- Time Perspective
- Prospective
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- Yes
Inclusion Criteria
- •Patients with positive findings during colonoscopy
Exclusion Criteria
- •Patients below 18 years old
- •Patients who cannot sign a consent form
Outcomes
Primary Outcomes
percentage of cells in G2/M arrest after dose-dependent exposure
Time Frame: 24 hours of exposure to strigolactone
analysis of cell cycle
percentage of cells with reduced viability after dose-dependent exposure
Time Frame: 24 hours of exposure to strigolactone
Secondary Outcomes
No secondary outcomes reported
Investigators
ido laish
MD
Meir Medical Center