IL-10-dependent immune suppression after trauma - regulation of monocytes and T-cells-controlled immune defense

Conditions: A41
A41.0
A41.9
J80
N17
K72.0
Other sepsis
Sepsis due to Staphylococcus aureus
Sepsis, unspecified
Adult respiratory distress syndrome

Registration Number: DRKS00003318

Lead Sponsor: Klinik für Unfall-, Hand- und WiederherstellungschirurgieKlinik der J W Goethe-Universität

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Pending

Sex: All

Target Recruitment: 140

Inclusion Criteria

all patients with an Injury Severity Score (ISS) between = 16 and ISS < 42, primary allocation after accident, preclinical time < 120 min, regular completion of the first treatment in the emergency room, signed informed consent

Exclusion Criteria

all patients with an ISS < 16 and ISS > 42, very poor prognosis/unfavourable prognosis (infaust prognosis) within first 24h after trauma, dementia or lacking cognitive abilities to participate the study (before trauma), patients with severe burn injuries, acute myocardial infarction, coronary bypass surgery, patients who are not primary allocated to the intensive care unit of the study center, tumor patients (chemotherapy,irradiation, HIV, Hepatitis A, B, C, HCV, CMV, immune suppressive drug medication

Study & Design

Study Type: observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Clinical: Diagnosed complication after trauma (sepsis, septic shock, organ failure, multiple organ failure, death)<br>Experimental: analysis of monocyte and regulatory t-cells function, daily in a time course of 10 days after trauma + determination of the cytokine profile in serum.<br><br>Monocytic functionality is evaluated by whole blood stimulation with lipopolysaccharide and subsequent detemination of cytokines in the supernatant (TNF-a, IL-1ß and IL-10) by ELISA method. Phagocytosis-activity of monocytes is determined by incubation of blood samples with FITC-stained E.coli (opsonized)-solution and subsequent flow cytometric analysis. <br>Determinatio of T-cell function is performed by stimulation with ConA and subsequent measurement of cytokines (IFN-?, IL-2, IL-4 und IL-10) in the supernatant.<br>

Secondary Outcome Measures