Regulation of Human Tendon and Skeletal Muscle Protein Turnover Following Tissue Injury: Mechanisms Behind Regeneration and Injury Preventive Adaptations
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 25
- 试验地点
- 2
- 主要终点
- Effect of somatropin on muscle protein synthesis 14 days after injury
研究概览
简要总结
The study is a 5-week human study including 24 18-35 year old healthy men. Each participant will have muscle injury and tendon strain induced in one leg using neuromuscular electrical stimulation in conjunction with forced lengthening contractions. The investigators will monitor the recovery from injury/strain over a 4 week period, in which half of the subjects will receive growth hormone (somatropin) to stimulate the connective tissue synthesis.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Triple (Participant, Investigator, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •Healthy men
- •BMI between 18.5-30 kg/m^2
排除标准
- •Regular strength training within the last 3 months
- •Current or former use of anabolic steroids or growth hormone
- •Use of corticosteroids in the last 3 months
- •Use of blood thinning medication
- •Use of medication which can affect muscle protein synthesis
- •Current or former drug og alcohol abuse
- •Knee pain
- •Previous participation in studies using deuterated water or alanine tracers
结局指标
主要结局
Effect of somatropin on muscle protein synthesis 14 days after injury
时间窗: 14 days
The difference in fractional synthesis rate in muscle proteins 14 days after injury with or without 14 days treatment with somatropin. Measured using deuterated water
次要结局
- Muscle protein breakdown 14 days after injury(14 days)
- Muscle protein synthesis 7 days after injury(7 days)
- Muscle protein synthesis 14 days after injury(14 days)
- Muscle protein breakdown 7 days after injury(7 days)
- Muscle protein breakdown 28 days after injury(28 days)
- Histochemical staining of muscle cross sections 7 days after injury(7 days)
- Histochemical staining of muscle cross sections 14 days after injury(14 days)
- Histochemical staining of muscle cross sections 28 days after injury(28 days)
- The effect of somatropin on tendon tissue synthesis(7 days)
- Tendon tissue synthesis 7 days after strain(7 days)
- Tendon tissue synthesis 7 days after strain with/without somatropin(7 days)
- Isometric muscle force output 7 days following injury(7 days)
- Isometric muscle force output 14 days following injury(14 days)
- Isometric muscle force output 28 days following injury(28 days)
研究者
Grith Højfeldt
Principal investigator, postdoc
Bispebjerg Hospital