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Testing the safety and immune response of HPV-DNA vaccination in patients with a HPV-positive preinvasive lesion of the vulva.

Completed
Conditions
VIN III lesion, immunotherapy, HPVVIN III laesie, immuuntherapie, HPV
Registration Number
NL-OMON27857
Lead Sponsor
KI-AV
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Completed
Sex
Not specified
Target Recruitment
12
Inclusion Criteria

* Age above 18 years

* Willing and able to undergo the planned study procedures

Exclusion Criteria

* Prior treatment with anti-HPV agents

* Participation in a study with another investigational drug within 30 days prior to the enrolment in this study

Study & Design

Study Type
Interventional
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
* To study the safety and toxicity of two different doses of the naked DNA vaccine encoding the shuffled HPV16 E7 gene products (TTFC-E7SH).<br /><br>* To study the HPV-specific immune response in two different doses of TTFC-E7SH.
Secondary Outcome Measures
NameTimeMethod
* To study the clinical response to vaccination of two different doses of TTFC-E7SH<br><br /><br /><br>Exploratory outcomes:<br /><br>* Local immune response<br /><br>HPV16-specific proliferative capacity will be tested in triplicate in a 3 day proliferation assay.<br /><br>* Tumour microenvironment<br /><br>The effect of vaccination on the tumour microenvironment will be determined by multicolour fluorescent immunohistochemistry.
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