eukocyte dynamics in health and disease: in vivo labelling of dividing leukocytes using deuterated water

Withdrawn

• Conditions: lymfopenie t.g.v. stamceltransplantatie; lymphopenia; immune cell loss; 10021460

• Registration Number: NL-OMON33161
• Lead Sponsor: niversitair Medisch Centrum Utrecht

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 6 May 2024

Recruitment & Eligibility

• Status: Withdrawn
• Sex: Not specified
• Target Recruitment: 60

Inclusion Criteria

a. healthy, 20-30 years of age, HIV-negative
b. healthy, 60 years or older, HIV-negative
c. healthy, 18 years or older, complete thymus removed at young age because of heart surgery
d. stem cell transplantation patiënt, 18 years or older, treated for hematological malignancies with an autologous HSCT, HIV-negative
e. 18 years or older, untreated HIV-1-infection

Exclusion Criteria

a, b, c. do not suffer from immunological disorders and show no obvious symptoms of infections
d. do not suffer from graft-versus-host-disease or severe (viral) infections
e. do not receive antiviral therapy

Study & Design

• Study Type: Observational invasive
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>The main parameter of the study is the amount of deuterium (label) that the<br /><br>different leukocyte populations have incorporated in their DNA by cell division<br /><br>at a given time. For this purpose blood withdrawals are done both in the period<br /><br>during which participants drink 2H2O (uplabelling phase), and in the period<br /><br>after stopping with 2H2O intake (delabelling phase). Data obtained during<br /><br>uplabelling and delabelling phases can be interpreted by mathematical models<br /><br>that describe the dynamics of leukocyte populations.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>n.a.</p><br>