Changes in intracellular metabolism and inflammatory function of circulating and adipose tissue depot-specific CD14+ cells in obesity and diabetes mellitus
Completed
- Conditions
- adipose tissue chronic low-grade inflammationinflammation of the adipose tissue10012653
- Registration Number
- NL-OMON42823
- Lead Sponsor
- Radboud Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 45
Inclusion Criteria
group 1 (males and females): age: 35-75 years, body mass index (BMI) of 19-25 kg/m2;group 2 (males and females): age: 35-75 years, body mass index (BMI) of >25 kg/m2 ;group 3 (males and females): age: 35-75 years, body mass index (BMI) of >25 kg/m2, diagnosis of T2DM
Exclusion Criteria
any presence of a chronic or acute inflammation and/or autoimmune disorder, any anti-inflammatory medication, metabolic diseases, endocrine diseases and chronic and/or acute inflammatory diseases (high sensitivity C-reactive protein > 1 mg/liter)
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>To determine metabolic pathways important for inflammatory function of CD14+<br /><br>cells from blood, visceral and subcutaneous adipose tissue by gene expression<br /><br>analysis (human microarray platform by affymetrix) using a bioinformatics<br /><br>approach.<br /><br><br /><br>To determine the intracellular metabolism of CD14+ cells from blood and adipose<br /><br>tissue by measurements of metabolites in cell lysates or supernatants of the<br /><br>(cultured) immune cells and by analysis of intracellular metabolic signalling<br /><br>pathways on protein and gene expression level.</p><br>
- Secondary Outcome Measures
Name Time Method <p>To determine the differences in inflammatory potential of CD14+ cells isolated<br /><br>from lean, obese and diabetic subjects by histological assessment of adipocyte<br /><br>size, macrophage infiltration and formation of crown-like structures in the<br /><br>different adipose tissue depots and by measurements of plasma cytokines and<br /><br>cytokines released upon stimulation of cultured cells isolated from the blood. </p><br>