Analysis of Gingival Crevicular Fluid IL-1β, IL-10, IL-36γ Cytokine Levels in Gingivitis and Periodontitis Patients
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 81
- 主要终点
- Interleukin-36γ (IL-36γ) levels in GCF
研究概览
简要总结
The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
详细描述
Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF.
The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).
研究设计
- 研究类型
- Observational
- 观察模型
- Other
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 20 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy
- •non-smokers,
- •presence of at least 20 teeth in the mouth
排除标准
- •receiving periodontal therapy in the last 6 months;
- •receiving antibiotics or anti-inflammatory drugs in the last 6 months;
- •currently pregnant or breastfeeding
- •using a removable partial denture and removable or fixed orthodontic appliances.
结局指标
主要结局
Interleukin-36γ (IL-36γ) levels in GCF
时间窗: Baseline
GCF samples were obtained from one interproximal site in each quadrant
次要结局
- Probing depth (PD)(Baseline)
- Bleeding on Probing (BoP)(Baseline)
- IL-10 and IL-1β levels in GCF(Baseline)
- Plaque Index (PI)(Baseline)
- Clinical attachment level (CAL)(Baseline)
- Gingival Index (GI)(Baseline)
研究者
Mehtikar Gursel
Prof.Dr
Bezmialem Vakif University