Analysis of Gingival Crevicular Fluid
- Conditions
- Periodontal Diseases
- Interventions
- Diagnostic Test: Gingival crevicular fluid sampling
- Registration Number
- NCT05325905
- Lead Sponsor
- Bezmialem Vakif University
- Brief Summary
The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
- Detailed Description
Gingival crevicular fluid (GCF) is formed when fluid exudes from the vessels of the microcirculation into the inflamed periodontal tissue and into the sulcus or pocket. As the fluid traverses the inflamed tissue, it is thought to pick up enzymes and other molecules that participate in the destructive process, as well as products of cell and tissue degradation. Efforts to develop diagnostic tests based on host factors have been focused almost entirely on analysis of GCF.
The purpose of this study was to investigate and compare GCF interleukin-1β (IL-1β), interleukin-10 (IL-10), interleukin-36γ (IL-36γ) levels in patients with healthy (H), gingivitis (G) and chronic periodontitis (CP) status.
Systemically healthy and non-smokers 80 participants (30 males and 50 females) who were selected from among admitting to Dentistry Faculty of Selcuk University were included in this study. Clinical periodontal parameters were recorded and GCF samples were obtained. Samples were stored in -80 ˚C until analysis with Enzyme-Linked ImmunoSorbent Assay (ELISA).
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 81
- Systemically healthy
- non-smokers,
- presence of at least 20 teeth in the mouth
- receiving periodontal therapy in the last 6 months;
- receiving antibiotics or anti-inflammatory drugs in the last 6 months;
- currently pregnant or breastfeeding
- using a removable partial denture and removable or fixed orthodontic appliances.
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Gingivitis Gingival crevicular fluid sampling gingival inflammation (BoP ≥ 10% of the sites, red-blue discoloration of the gingiva and edema) PD ≤ 4 mm and no clinical attachment or radiographic bone loss Periodontitis Gingival crevicular fluid sampling PD ≤ 5 mm, radiographic bone loss, and 3-4 mm CAL in at least 30% of the sites Healthy Gingival crevicular fluid sampling bleeding on probing (BoP) ≤ 10% of the sites, probing depth (PD) ≤ 3 mm and no indication of clinical attachment level (CAL) or radiographic bone loss
- Primary Outcome Measures
Name Time Method Interleukin-36γ (IL-36γ) levels in GCF Baseline GCF samples were obtained from one interproximal site in each quadrant
- Secondary Outcome Measures
Name Time Method Probing depth (PD) Baseline PD; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars.
Bleeding on Probing (BoP) Baseline BoP, from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars
IL-10 and IL-1β levels in GCF Baseline GCF samples were obtained from one interproximal site in each quadrant
Plaque Index (PI) Baseline PI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars
Clinical attachment level (CAL) Baseline CAL; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars.
Gingival Index (GI) Baseline GI; from six sites (mesio-buccal, mid-buccal, disto-buccal, mesio-lingual, mid-lingual, disto-lingual locations) of each tooth other than third molars