Age-related Changes in Sleep-wake Regulation

Not Applicable

Completed

• Conditions: Sleep Deprivation
• Interventions: Behavioral: Prolonged wakefulness

• Registration Number: NCT03813082
• Lead Sponsor: University of Zurich

Brief Summary

A sleep deprivation protocol combined with state-of-the-art, simultaneous positron emission tomography and magnetic resonance spectroscopy imaging will be employed to investigate the effects of sleep deprivation and aging on hypothesized molecular markers of sleep need.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-03-01
• Primary Completion: 2017-09
• Study First Submitted: 2019-01-17
• Study First Submitted QC: 2019-01-22
• Study First Posted: 2019-01-23
• Study Completion: 2019-12
• Status Verified: 2021-01
• Last Update Submitted: 2021-01-25
• Last Update Posted: 2021-01-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 29

Inclusion Criteria

• healthy male individuals
• age: 20-35 or 60-70 years
• right-handed
• non-smokers
• moderate caffeine/alcohol consumption
• normal BMI

Exclusion Criteria

• Sleep/circadian rhythms disturbances
• former brain injuries with loss of consciousness and brain anomalies
• drug intake/consumption
• medication intake
• cardiac pacemaker
• neurological disorders
• prior participation (during past 2 years) in radiological or nuclear experiment

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Sleep deprivation young men	Prolonged wakefulness	Young study participants will complete four nights in the sleep laboratory, whereas they will stay awake during one night. PET brain imaging will be conducted at the same circadian time on three consecutive afternoons (prior, during and after prolonged wakefulness). Additionally, validated tests of vigilance and cognitive performance will be administered and the brain waves will be recorded in wakefulness and sleep.
Sleep deprivation older men	Prolonged wakefulness	Older study participants will complete four nights in the sleep laboratory, whereas they will stay awake during one night. PET brain imaging will be conducted at the same circadian time on three consecutive afternoons (prior, during and after prolonged wakefulness). Additionally, validated tests of vigilance and cognitive performance will be administered and the brain waves will be recorded in wakefulness and sleep.

Primary Outcome Measures

Name	Time	Method
Sleep-wake induced change in cerebral availability of mGluR5 (metabotropic glutamate receptors of subtype 5)	Change from baseline metabotropic glutamate receptors of subtype 5 availability after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	Positron emission tomography

Secondary Outcome Measures

Name	Time	Method
Sleep-wake induced change in waking electroencephalogram (EEG)	Change from baseline waking EEG during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Spectral composition of the EEG in wakefulness
Sleep-wake induced change in subjective state	Change from baseline sleepiness during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Subjective sleepiness as quantified with the Stanford Sleepiness Scale (range: 1 to 7)
Sleep-wake induced change in cognitive performance	Change from baseline cognitive performance during 33 hours of prolonged wakefulness and after 8 hours of recovery sleep	Cognitive performance battery including tasks measuring sustained attention, executive functioning and working memory
Sleep-wake induced change in potential molecular markers of sleep need	Change from baseline microRNA, fragile X mental retardation protein and brain-derived neurotrophic factor concentrations after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	microRNAs, fragile X mental retardation protein, and brain-derived neurotrophic factor concentrations in peripheral blood
Sleep-wake induced change in glutamate concentration in prefrontal cortex and basal ganglia	Change from baseline glutamate concentration after 33 hours of prolonged wakefulness and 8 hours of recovery sleep	Magnetic resonance spectroscopy
Sleep-wake induced change in sleep electroencephalogram (EEG)	Change from all-night baseline sleep EEG in 8 hours of recovery sleep after 33 hours of prolonged wakefulness	Spectral composition of the EEG during sleep