The Impact of Hypoxia on the Biochemical and Morphological Parameters of Blood in Rowers.

Not Applicable

Completed

• Conditions: Hypoxia
• Interventions: Other: hypoxia

• Registration Number: NCT06264193
• Lead Sponsor: Poznan University of Physical Education

Brief Summary

The response to hypoxia is very individual and epending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that 18 days of the LH-TL method does not significantly increase the level of EPO and VEGF in rowers. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Detailed Description

The Polish national rower team athletes, 13 males (ages 27,0±4,2 years), were randomly divided into two groups: 1) The hypoxic group (H) included 8 rowers participating in sports training and the LH-TL method, hypoxic rooms (FiO2=14.5%, corresponds to an altitude of 3000 meters). 2) The control group (C) presented 5 rowers participating only in sports training and living in normoxic rooms, at the same sports camp. Rowers were observed during the preparatory period. The hypoxic group lived and slept in hypoxic rooms, for at least 8-14 hours per day. During the camp, all rowers followed the same training schedule and diet.All participants' saturation and heart rate were measured daily, morning and evening, throughout the camp period. Venous blood samples were taken four times, at the beginning of the camp (Baseline), after 5 days, after 12 days, and after 18 days of the camp. Body composition parameters such as body mass index, body fat, and free fat mass were estimated using the bioelectrical impedance method of an analyzer Tanita BC 418 (Japan). Data analysis was performed using Statistica. The response to hypoxia is very individual. Depending on many aspects, such as the type of training, duration, intensity, or hypoxic stimulus, hypoxia affects the athlete in various ways. The results of this study have shown that the blood parameters did not increase significantly in the hypoxic group compared to the control group. However, reticulocytes, immature red blood cells, have shown significant differences after 18-d LH-TL between groups. Further research should be carried out to investigate an optimal hypoxic dose and time, which will raise EPO, VEGF, and morphology variables.

Study Timeline

• Study Start: 2014-11-30
• Primary Completion: 2014-12-21
• Study Completion: 2014-12-21
• Status Verified: 2023-11
• Study First Submitted: 2024-01-10
• Study First Submitted QC: 2024-02-09
• Study First Posted: 2024-02-16
• Last Update Submitted: 2025-01-15
• Last Update Posted: 2025-01-17

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 13

Inclusion Criteria

• member of national rower's teams, and sports camp participant.

Exclusion Criteria

• negative symptoms of hypoxia, and/or injury.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
Hypoxia	hypoxia	live high-train low method was used

Primary Outcome Measures

Name	Time	Method
Erythropoietin (EPO) mlU/ml	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	immunoenzymatic assay methods using diagnostic kits
vascular endothelial growth factor (VEGF) mlU/ml	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	immunoenzymatic assay methods using diagnostic kits

Secondary Outcome Measures

Name	Time	Method
hemoglobin (Hb)g/dL	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	A professional accredited laboratory company determined the Hb level (Poland, ISO 9001:2008).
bpm - beats per minute(%)	every day, morning and evening, while sports camp	was measured using a pulse oximeter (Tech-Med, ISO: 13485).
creatine kinase (CK) ng/ml	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	was determined using spectrophotometer analysis and diagnostic kits
Hs C-Reactive Protein (hsCRP) mg/L	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	was determined by immunoenzymatic assay methods using diagnostic kits
hematocrit (Htc)%	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	A professional accredited laboratory company determined the Htc level (Poland, ISO 9001:2008).
white blood cells (WBC)10 3/µL	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	A professional accredited laboratory company determined the WBC level (Poland, ISO 9001:2008).
SpO2 - oxygen saturation (%)	every day, morning and evening, while sports camp	was measured using a pulse oximeter (Tech-Med, ISO: 13485).
red blood cells (RBC)mln/mm3	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	A professional accredited laboratory company determined the RBC level (Poland, ISO 9001:2008).
reticulocytes (Ret)‰	At the beginning of the camp (Baseline), after 5 days (T1), after 12 days (T2), and after 18 days (T3) of the camp.	A professional accredited laboratory company determined the Ret level (Poland, ISO 9001:2008).