Molecular Mechanisms Underpinning Sarcopenic Obesity
试验速览
- 阶段
- 不适用
- 状态
- 招募中
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Fiber type-specific cell signalling
研究概览
简要总结
The coexistence of obesity and sarcopenia results in sarcopenic obesity, a high-risk geriatric condition associated with metabolic perturbations and several co-morbidities. Despite the rising numbers of older adults diagnosed with sarcopenic obesity, few studies have characterized this condition on the muscle cell and systemic level. This study sought to comprehensively assess the influence of sarcopenic obesity on muscle and blood-related parameters and compare it to non-sarocopenic obese and non-sarcopenic lean older adults.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 80 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Body fat % in accordance with previously defined criteria
- •Free of smoking (since 10 years)
- •Able to walk without assistance
- •Able to perform bilateral leg extension exercise
排除标准
- •History of or present disease of myocardial, vascular, endocrine, hepatic, renal, pulmonary or neuromuscular origin
- •Uncontrolled hypo/hypertension
- •Use of anticoagulants (Warfarin, Trombyl)
- •Use of metformin or other anti-diabetic medications
- •Poor appetite and unexplained weight loss (4,5 kg) over the past 6 months
- •Active malignancy
- •Present infection
- •Dementia or delirium
- •Other conditions that may interfere with the study protocol in the opinion of the PI
结局指标
主要结局
Fiber type-specific cell signalling
时间窗: 1 hour after ingestion of essential amino acids and resistance exercise
This measure will be investigated during an experimental trial in which the participants are subjected to an acute bout of resistance exercise followed by the ingestion of essential amino acids (240 mg/kg lean mass). During this 3 hour time period, the participants will donate two muscle biopsies, one before exercise and the second one 1 hour after exercise. Cell signalling networks related to muscle mass regulation will be examined in these biopsy samples. The signal will be measured using Western blotting and the signal obtained is expressed in relation to baseline (adjusted volume intensity).
次要结局
- Whole body glucose tolerance(2 hours)
- Accelerometer-assessed physical activity levels(1 week)
- Isometric and isokinetic leg muscle strength(1 hour)
- Skeletal muscle fiber characteristics(1 hour prior to ingestion of essential amino acids and resistance exercise)
- Whole body muscle volume(1 hour)
研究者
William Apro
Associate Professor
The Swedish School of Sport and Health Sciences