Serum Level of IL31,33 and 36 in Egyptian Paients With Psoriasis in Assiut University Hospital : Case Control Study
试验速览
- 阶段
- 不适用
- 入组人数
- 90
- 主要终点
- describe the serum levels of IL31, IL33 and IL36 in egyptian patients with psoriasis.
研究概览
简要总结
Evaluating Serum Level Of IL 31 , IL33 and IL36 and Their Correlation with Disease Activity In Patients With Psoriasis In Assuit University Hospital .
- To correlate their levels with disease activity using PASI score .
- To allow better understanding of the pathophysiological mechanism of the disease .
详细描述
Serum samples will be collected from 45 psoriasis patients and 45 matched healthy individuals. After full history taking and dermatological examination, the disease severity will be assessed using the psoriasis area and severity index (PASI) score. All patients enrolled in our study had no other autoimmune or systemic diseases and underwent no systemic treatment including glucocorticoids, immunosuppressive drugs, or phototherapy at least 1 month before the PASI score evaluation and sample collection period. Wriiten informed consent will be obtained from all the patients and healthy controls.
Assessment of serum interleukin-31 ,33 and 36 concentrations in psoriatic patients Patients and controls Blood samples will collected from psoriatic patients and controls, and will centrifuged for 15 min at 1000 rpm. Next, the serum samples will be subdivided into small aliquots to be stored at -80°C until analysis for cytokine levels. ELISA kits will be used to determine serum IL-31 , 33 and 36
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Retrospective
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients with all types of psoriasis.
- •healthy volunteers without a family history of psoriasis or other autoimmune disease.
排除标准
- •other autoimmune , other chronic inflammatory or systemic diseases.
- •cases received glucocorticoids, immunosuppressive drugs at least 1 month before sample collection .
结局指标
主要结局
describe the serum levels of IL31, IL33 and IL36 in egyptian patients with psoriasis.
时间窗: Baseline
1. Blood samples will collected from psoriatic patients and controls. 2. using ELISA kits to measure serum concentration of IL31 \[pg/ml\] , IL33 \[pg/ml\] and IL36 \[pg/ml\] . 3. p value less than 0,05 reflect significant increase in serum levels of measured interleukins.
4. Knowing the extent of disease activity by Psoriasis Area and Severity Index (PASI) score.
时间窗: Baseline
assessment of the severity of lesions and the area affected into a single score in the range 0 (no disease) to 72 (maximal disease).
次要结局
- serum level of interleukins reflect severity of disease and represent anovel target for antipsoriatic drug theraby.(Baseline)
研究者
Fatma Elzahraa Adel Ismail
Principal Investigator
Assiut University