Evaluation of Interleukin (IL)-38, IL-36-gamma, IL-17 and Matrix Metalloproteinase-9 Levels in Gingival Crevicular Fluid, Saliva and Serum for Periodontal Health, Gingivitis and Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 90
- 试验地点
- 2
- 主要终点
- Levels of Interleukin-36 gamma (pg/ml) in GCF, saliva and serum fluid
研究概览
简要总结
The aim of this study is; detection of interleukin(IL)-38, IL-36 gamma(γ) , IL-17 and matrix metalloproteinase(MMP)-9 levels in gingival crevicular fluid (GCF), saliva and serum samples of periodontally healthy, gingivitis and periodontitis patients and the possible correlation between these values and clinical parameters of periodontal diseases. Materials and methods: Samples were obtained from 90 systemically healthy non-smoker individuals with periodontitis (P, n=30), gingivitis(G, n=30) and healthy periodontium (S, n=30). Full-mouth clinical periodontal measurements including probing depth (PD), clinical attachment level (CAL), bleeding on probing (BOP), gingival index (GI) and plaque index (PI) were also recorded. Enzyme-linked immunosorbent assay (ELISA) was used to determine IL-38, IL-36γ, IL-17 and MMP-9 levels in the biological samples.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Parallel
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Systemically healthy (The determination of healthy volunteers will be based on the statements of the patients in the anamnesis. No additional examinations will be made.)
- •At least twenty permanent teeth in the mouth
- •Non-smoker
- •No medication for continuous use
- •Those who have not used antibiotics, anti-inflammatory and systemic corticosteroid drugs in the last 6 months.
- •Not in pregnancy or lactation period.
- •For the periodontitis group that has not received periodontal treatment in the last 6 months
排除标准
- •Any oral or systemic disease
- •Regularly using a systemic medication
- •During pregnancy or lactation
- •Received periodontal treatment within the last 6 months.
- •Those who received antibiotic, anti-inflammatory or systemic corticosteroid medication in the last 6 months
结局指标
主要结局
Levels of Interleukin-36 gamma (pg/ml) in GCF, saliva and serum fluid
时间窗: 24 hours after taking the clinical measurements at the first visit
Levels of Interleukin-38 (pg/ml) in GCF, saliva and serum fluid
时间窗: 24 hours after taking the clinical measurements at the first visit
次要结局
- Levels of Interleukin-17 (pg/ml) in GCF, saliva and serum fluid(24 hours after taking the clinical measurements at the first visit)
- Levels of Matrix Metalloproteinase-9 (ng/ml) in GCF, saliva and serum fluid(24 hours after taking the clinical measurements at the first visit)
研究者
Hilal Korkmaz
Research Assistant
Akdeniz University