Impact of Inulin on Production of Phenolic Acids From Tomato Onion and Lovage Soup
- Conditions
- Healthy Adults
- Interventions
- Dietary Supplement: Tomato, onion & lovage soupDietary Supplement: Tomato, onion & lovage soup with inulinDietary Supplement: Inulin
- Registration Number
- NCT03577145
- Lead Sponsor
- University of Glasgow
- Brief Summary
This is an acute human bioavailability study in self-reported healthy participants aged 20-70 years old. The investigators hypothesize that combination of polyphenolics from a soup rich in rutin and quercitin and the non-digestible carbohydrate (NDC) inulin will increase the production of phenolic acids by bacteria in the human colon and these will be detected in urine. Participants will attend for three arms in a randomized order: Tomato, onion and lovage soup (high polyphenol food), Inulin (NDC) or Mixture of tomato, onion and lovage soup and inulin.
During each feeding study, urine, blood and stool samples will be collected at regular intervals for the duration of 24 hrs after consumption of test food. Participants will be asked to follow a low polyphenol diet for 2 days prior to the feeding study.
- Detailed Description
Polyphenol rich plant foods have been associated with several health benefits but their bioavailability is generally low. The majority of plant polyphenols are poorly absorbed in the small intestine and enter the colon where the colonic microbiota metabolise them to release a range of phenolic acids, which are now thought to be the main bioactive components related to the reduction in disease risk. Very little is known about the impact of other constituents of the diet on the metabolism and bacterial catabolism of these polyphenols.
Colonic bacteria are key agents in the release of the bioactive molecules from polyphenols but also ferment non-digestible carbohydrates (NDC) such as inulin to short chain fatty acids. It is likely that there are key interactions in the colonic bacterial metabolism of NDC and phenolics. The investigators hypothesize that combination of polyphenolics (in onions, tomatoes and lovage) with inulin (NDC) will increase the urinary output of bioactive phenolic acids.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 15
• Self-reported healthy adults
- Antibiotic use within the last 3 months
- Identified gastro-intestinal diseases
- On prescribed medication other than the contraceptive pill
- Pregnant or breastfeeding.
- Diagnosed as anaemic
- Allergic to paracetamol or any food
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Tomato, onion & lovage soup Tomato, onion & lovage soup One dose of tomato (300g), onion (100g) \& lovage (20g) will be given to subjects in the form of a soup Tomato, onion & lovage soup with inulin Tomato, onion & lovage soup with inulin One dose of tomato (300g), onion (100g) \& lovage (20g) with 10g inulin will be given to subjects in the form of a soup Inulin Inulin One dose of 10g inulin will be given to subjects in the form of a drink
- Primary Outcome Measures
Name Time Method Phenolic acids bioavailability 0-24 hrs Urine excretion of phenolic acids (µg) will be measured with GC-MS
- Secondary Outcome Measures
Name Time Method Mouth to caecum transit time 0-8 hrs Mouth to caecum transit time (in hours/mins) will be calculated from sustained rise in breath hydrogen level measurements by hydrogen monitor
Gastric emptying time 0-6 hrs Gastric emptying time (in hours/mins) will be estimated using kinetics of plasma paracetamol levels measured by acetaminophen assay kits
Glycaemic measurements 0-8 hrs Plasma glucose (mmol/L), insulin (mU/L) will be measured by commercial kits
Appetite hormones measurements 0-8 hrs Appetite hormone (PYY) levels in plasma (pg/mL) will be measured by commercial Elisa kit
Trial Locations
- Locations (1)
School of Medicine, Nursing and Dentistry, College of MVLS, University of Glasgow
🇬🇧Glasgow, Lanarkshire, United Kingdom