Effect of Platelet-Rich Plasma on the rate of Orthodontic Tooth Movement and on the Biomarkers present in Gingival Crevicular Fluid â€“ A Split Mouth Randomised Controlled Trial

Phase 2

Not yet recruiting

Conditions: Volunteers with dental malocclusion of protruding upper and lower teeth who need premolar extraction. participants between the ages of 16 years and 35years who has good periodontal health and no systemic medical history or metabolic bone disease were chosen for the study.
Disorder of teeth and supporting structures, unspecified,

Registration Number: CTRI/2023/04/051626

Lead Sponsor: Dr DY Patil Dental College

Brief Summary: Orthodontic Treatment requires the use of brackets and wires tofacilitate tooth movement and bring about alignment. This procedure takes anaverage of about 24 months and sometimes more. There have been various studiesto reduce the treatment time and many methods have been put forth to bringabout faster tooth movement. These include acceleration of orthodontic toothmovement by use of ultrasonic vibrations, photobiomodulation, low level lasertherapy, and use of pharmaceutical approach. One of the recently used localagents to accelerate the rate of orthodontic tooth movement is platelet-richplasma (PRP). PRP is defined as an autologous concentration of platelets in aminute volume of plasma. The alpha granules in the platelets are the mostabundant secretory granules. They contain numerous proteins, including growthfactors and chemokines, which are crucial for primary hemostasis and woundhealing. Additionally, the rate of orthodontic movement can be studied throughthe biomarkers present in the Gingival Crevicular Fluid(GCF) which is agingival exudate. Using the knowledge of the biochemical changes taking placein the GCF following the use of PRP, we can attempt to synthetically alter thetooth movement and accelerate our treatment time.

Novelty: We plan to use the information in accelerating orthodontic treatmenttime in adults so that more adult patients can seek orthodontic treatment.

Objectives: The studyâ€™s objective is to find the effect of PRP on therate of tooth movement and on the biomarkers that are present in GCF.

Methods: The method would include injecting a small amount of PRP which would beprepared from the patientâ€™s own blood, into the desired site in the mouth.Later, GCF samples would be collected from these sites at designated time intervalsto study the effect of PRP on the biomarkers in GCF.

Expected outcome:

1)Intervention side tooth movement will be accelerated.

2)Identification of biomarkers responsible for enhanced tooth movement.

3) Isolated Biomarkers for faster tooth movement therapy.

Detailed Description: Not available

Recruitment & Eligibility

Status: Not Yet Recruiting

Sex: All

Target Recruitment: 20

Inclusion Criteria

20 participants between the ages of 16 and 35 who have a full complement of teeth and good periodontal health and no severe medical history or metabolic bone disease will be chosen for the study.
The selected subjects either had bimaxillary protrusion or Class II Division I malocclusion, undergoing orthodontic treatment and requiring extraction of 1st premolars.

Exclusion Criteria

Exclusion criteria for the trial will be those with periodontal diseases/poor oral hygiene, patients with autoimmune diseases, pregnancy, and lactation and those on medication (e.g. antibiotics, antihistamines, cortisone, hormones, and any other medication that may interfere with the inflammatory process or adversely affect the periodontium) and syndromic patients.

Study & Design

Study Type: Interventional

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
INCREASED RATE OF TTOTH MOVEMENT BY ATLEAST 1.5% THAN NORMAL DUE TO THE EFFECT OF PRP	We intend to check the amount of tooth movement at 1, 2 and 3 months time interval

Secondary Outcome Measures

Name	Time	Method
EFFECT OF BIOMARKERS IN THE GCF COLLECTED DURING THE 3 MONTHS OF TRIAL PERIOD	3 MONTHS

Trial Locations

Locations (1): Dr. D.Y Patil Dental College and Hospital
🇮🇳
Pune, MAHARASHTRA, India
Dr. D.Y Patil Dental College and Hospital
🇮🇳Pune, MAHARASHTRA, India
Dr Sonali Deshmukh
Principal investigator
9371009387
sonalivdeshmukh@gmail.com