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Efficacy and Safety of Intramuscular PDA-002 in Subjects Who Have Diabetic Foot Ulcer With and Without Peripheral Arterial Disease

Phase 2
Terminated
Conditions
Peripheral Arterial Disease
Diabetic Foot
Interventions
Other: Placebo
Biological: 3 x 10^6 cells
Biological: 10 x 10^6 cells
Biological: 30 x 10^6 cells
Registration Number
NCT02264288
Lead Sponsor
Celularity Incorporated
Brief Summary

The PDA-002-DFU-002 trial is a Phase 2, multicenter, randomized, double blind, placebo controlled, dose range finding study. The study will enroll approximately 133 subjects in four treatment groups. The primary objective of the study is to assess the efficacy and safety of PDA-002 administered intramuscular (IM) in subjects who have DFU with and without PAD. The secondary objective is to explore potential clinical efficacy by assessing changes in vascular parameters such as Ankle-Brachial Index and Toe-Brachial Index (ABI and/or TBI), Transcutaneous oxygen measurements (TcPO2).

Detailed Description

Not available

Recruitment & Eligibility

Status
TERMINATED
Sex
All
Target Recruitment
159
Inclusion Criteria
  1. Males and females, at least 18 years of age or older at the time of signing the informed consent document.
  2. Understand and voluntarily sign an informed consent document prior to any study related assessments/procedures are conducted.
  3. Able to adhere to the study visit schedule and other protocol requirements.
  4. Diabetes mellitus Type 1 or Type 2.
  5. Diabetic foot ulcer with severity of Grade 1 (full thickness only) or Grade 2 on the Wagner Grading Scale (Appendix A) of greater than one month duration which has not adequately responded to conventional ulcer therapy with a size of at least of 1cm2 except if present on the toe. The maximum lesion size range in the index ulcer is ≤ 10cm2. The measurement of the index ulcer is to be evaluated and measured after debridement (if necessary) at the Screening Visit. If located on the plantar aspect of the foot, the index ulcer must be able to be adequately offloaded in the assessment of the investigator.
  6. No planned revascularization or amputation over the next 3 months after Screening visit, in the opinion of the Investigator.
  7. Screening should not begin until at least 14 days after a failed reperfusion intervention and at least 30 days after a successful reperfusion intervention.
  8. Subjects should be receiving appropriate medical therapy for hypertension and diabetes any other chronic medical conditions for which they require ongoing care.
  9. A female of childbearing potential must have a negative serum pregnancy test at Screening and a negative urine pregnancy test prior to treatment with study therapy. In addition, sexually active FCBP must agree to use 2 of the following adequate forms of contraception methods simultaneously such as: oral, injectable, or implantable hormonal contraception; tubal ligation; IUD; barrier contraceptive with spermicide or vasectomized partner for the duration of the study and the Follow-up Period.
  10. Males (including those who have had a vasectomy) must agree to use barrier contraception (latex condoms) when engaging in reproductive sexual activity with FCBP for the duration of the study and the Follow-up Period.
Exclusion Criteria
  1. Any significant medical condition, laboratory abnormality, or psychiatric illness that would prevent the subject from participating in the study.
  2. Any condition including the presence of laboratory abnormalities, which places the subject at unacceptable risk if he or she were to participate in the study.
  3. Any condition that confounds the ability to interpret data from the study.
  4. Pregnant or lactating females.
  5. Subjects with a body mass index > 45 kg/m2 at Screening.
  6. AST (SGOT) or ALT (SGPT) > 2.5 x the upper limit of normal (ULN) at Screening.
  7. Patient on renal dialysis for abnormal kidney function.
  8. An ABI < 0.4 and or TBI < 0.3 in the leg with the index ulcer.
  9. Bilirubin level > 2 mg/dL (unless subject has known Gilbert's disease) at Screening.
  10. Untreated chronic infection or treatment of any infection with systemic antibiotics, including the ulcer site, must be free of antibiotics within 1 week prior to dosing with IP.
  11. Active osteomyelitis, infection, or cellulites at or adjacent to the index ulcer. Patients with a history of being treated for an osteomyelitis without a surgical resection.
  12. Index ulcer that has decreased or increased in size by ≥ 30% during the Screening/Run-In/ Pre-Treatment Period.
  13. Active Charcot Neuroarthropathy in the foot with the index ulcer
  14. Pain at rest due to limb ischemia.
  15. Uncontrolled hypertension (defined as diastolic blood pressure > 100 mmHg or systolic blood pressure > 180 mmHg during Screening at 2 independent measurements taken while subject is sitting and resting for at least 5 minutes).
  16. Poorly controlled diabetes mellitus (hemoglobin A1c > 12% or a screening serum glucose of ≥ 300mg/dl).
  17. Untreated proliferative retinopathy.
  18. History of malignant ventricular arrhythmia, CCS Class III-IV angina pectoris, myocardial infarction/ percutaneous coronary intervention (PCI) / coronary artery bypass graft (CABG) in the preceding 6 months prior to signing the informed consent form (ICF), pending coronary revascularization in the following 3 months, transient ischemic attack/cerebrovascular accident in the preceding 6 months, prior to signing the ICF, and/or New York Heart Association [NYHA] Stage III or IV congestive heart failure.
  19. Abnormal ECG: new right bundle branch block (BBB) ≥ 120 msec in the preceding 3 months prior to signing the ICF.
  20. Uncontrolled hypercoagulation syndrome.
  21. Life expectancy less than at 2 years at the time of signing the ICF due to concomitant illnesses.
  22. In the opinion of the Investigator, the subject is unsuitable for cellular therapy.
  23. History of malignancy within 5 years prior to signing the ICF except basal cell or squamous cell carcinoma of the skin or remote history of cancer now considered cured or positive Pap smear with subsequent negative follow-up.
  24. History of hypersensitivity to any of the components of the product formulation (including bovine or porcine products, dextran 40, and dimethyl sulfoxide [DMSO]).
  25. Subject has received an investigational agent -an agent or device not approved by the US Food and Drug Administration (FDA) for marketed use in any indication- within 90 days (or 5 half-lives, whichever is longer) prior to treatment with study therapy or planned participation in another therapeutic study prior to the completion of this study.
  26. Subject has received previous investigational gene or cell therapy.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboIdentically matching placebo administered IM on Study Days 1 and 8
3 x 10^6 cells3 x 10^6 cellsHuman Placenta Derived cells (PDA-002) administered intramuscularly (IM) on Study Days 1 and 8
10 x 10^6 cells10 x 10^6 cells10 x 10\^6 Human Placenta Derived cells (PDA-002) administered intramuscularly on Study Days 1 and 8
30 x 10^6 cells30 x 10^6 cells30 x 10\^6 Human Placenta Derived cells (PDA-002) administered intramuscularly on Study Days 1 and 8
Primary Outcome Measures
NameTimeMethod
Complete wound closure of the index ulcerUp to 4 months

Complete wound closure is defined as closure of the index ulcer and retaining wound closure for the subsequent 4 weeks

Safety (adverse events [type, frequency, and severity of AEs, and relationship of AEs to study drug], laboratory abnormalities, and hospitalizationsUp to 24 months

An AE is any noxious, unintended, or untoward medical occurrence that may appear or worsen in a subject during the course of a study.

Secondary Outcome Measures
NameTimeMethod
Time to ulcer closureUp to 6 months

The time period for the index ulcer to close

Ankle Brachial Index (ABI ) and Toe-Brachial Index (TBI)Up to 6 months

ABI and TBI will be calculated by dividing the systolic blood pressure at the ankle or toe by the systolic blood pressures (Doppler technique) in the arm

50% Closure of Index UlcerUp to 24 months

The proportion of subjects with at least 50% closure of the index ulcer

Transcutaneous oxygenUp to 6 months

Transcutaneous oxygen measurements determine the oxygen level in tissue below the skin

Time to amputation, ulcer opening closing, gangrene and infectionUp to 24 months

Time to major amputation (above the ankle) of treated leg, minor amputations, to re-opening of ulcer, time to doubling / halving of index ulcer total surface area from baseline, de novo gangrene and foot wound infection

Wagner Grading ScaleUp to 24 months

The Wagner system assesses ulcer depth and the presence of osteomyelitis or gangrene by using the following grades: grade 0 (pre-or postulcerative lesion), grade 1 (partial/full thickness ulcer), grade 2 (probing to tendon or capsule), grade 3 (deep with osteitis), grade 4 (partial foot gangrene), and grade 5 (whole foot gangrene).

Rutherford CriteriaUp to 6 months

The Rutherford classification is a clinical staging system for describing peripheral arterial disease.

36-item Short Form Health Survey (SF-36)Up to 6 months

A validated measurement of health related quality of life

Diabetic Foot Ulcer Scale Short Form (DFS-SF) index ulcerUp to 6 months

A measurement of the impact of diabetic foot ulcer on a patient's quality of life.

Patient Global Impression of Change in Neuropathy (PGICN)Up to 24 months

Assessment of changes in neuropathy over time

European Quality of Life: EuroQOL-5D- health utility index assessment (EQ-5D)Up to 6 months

A standardized instrument used as a measure of health outcome

Number of all ulcersUp to 24 months

The total number of ulcers

Leg rest pain scoreUp to 24 months

A visual analog scale (VAS) graded from 0 (pain free) to Grade 10 (maximum pain)

Trial Locations

Locations (55)

UCLA

🇺🇸

Los Angeles, California, United States

University of Pennsylvania Health Systems

🇺🇸

Philadelphia, Pennsylvania, United States

Premier Vein and Vascular Center

🇺🇸

Houston, Texas, United States

Clinical Research of West Florida Inc - Clearwater

🇺🇸

Clearwater, Florida, United States

East Valley Foot and Ankle Specialist

🇺🇸

Mesa, Arizona, United States

Limb Preservation Platform, INC.

🇺🇸

Fresno, California, United States

TFi Medical

🇺🇸

Mobile, Alabama, United States

Aung Foothealth Clinics and Advanced Wound Care Center

🇺🇸

Tucson, Arizona, United States

Reliance Clinical Research

🇺🇸

Chino, California, United States

Center for Clinical Research Inc.

🇺🇸

Castro Valley, California, United States

Jeffrey A Klemes DPM

🇺🇸

Beverly Hills, California, United States

Foot and Ankle Clinic

🇺🇸

Los Angeles, California, United States

VA Palo Alto Health Care System

🇺🇸

Palo Alto, California, United States

Stanford University

🇺🇸

Stanford, California, United States

Georgetown University Medical Center Lombardi Cancer Center

🇺🇸

Washington, District of Columbia, United States

Barry University

🇺🇸

Fort Lauderdale, Florida, United States

The Research Center

🇺🇸

Hialeah, Florida, United States

Solutions Through Advanced Research Inc.

🇺🇸

Jacksonville, Florida, United States

University of Miami

🇺🇸

Miami, Florida, United States

GF Professional Research Group Corporation

🇺🇸

Miami Lakes, Florida, United States

University of Illinois at Chicago Cancer Center

🇺🇸

Chicago, Illinois, United States

Well Pharma Medical Research Corporation

🇺🇸

Miami, Florida, United States

Med-Care Research Inc

🇺🇸

North Miami Beach, Florida, United States

Podiatry 1st

🇺🇸

Belleville, Illinois, United States

Weill Foot & Ankle Institute

🇺🇸

Des Plaines, Illinois, United States

Southern Illinois University

🇺🇸

Springfield, Illinois, United States

Rosalind Franklin University of Medicine and Science

🇺🇸

North Chicago, Illinois, United States

Sinai Hospital of Baltimore

🇺🇸

Baltimore, Maryland, United States

Revive Research Institute

🇺🇸

Sterling Heights, Michigan, United States

Northwest Hospital

🇺🇸

Randallstown, Maryland, United States

Office of Michael J. De Marco, DPM

🇺🇸

Tinton Falls, New Jersey, United States

Ocean City Foot and Ankle Assoc

🇺🇸

Toms River, New Jersey, United States

University of Texas Southwestern Medical Center

🇺🇸

Dallas, Texas, United States

Clinical Research Associates of Central Pennsylvania

🇺🇸

Duncansville, Pennsylvania, United States

Syracuse VA Medical Center

🇺🇸

Syracuse, New York, United States

Complete Family Foot Care - McAllen Office

🇺🇸

McAllen, Texas, United States

Carilion Clinic

🇺🇸

Roanoke, Virginia, United States

1Foot 2Foot Centre for Foot & Ankle Care PC

🇺🇸

Suffolk, Virginia, United States

Milwaukee Foot & Ankle Specialists

🇺🇸

Wauwatosa, Wisconsin, United States

Carl T. Hayden Veterans Affairs Medical Center

🇺🇸

Phoenix, Arizona, United States

Arizona Arthritis and Rheumatology Research, PLLC

🇺🇸

Phoenix, Arizona, United States

Endeavor Clinical Trials PA

🇺🇸

San Antonio, Texas, United States

SAM Clinical Research Center

🇺🇸

San Antonio, Texas, United States

Advanced Foot & Ankle Center

🇺🇸

Salt Lake City, Utah, United States

Cardiology PC

🇺🇸

Birmingham, Alabama, United States

CGH Medical Center Main Clinic

🇺🇸

Sterling, Illinois, United States

Northeast Arkansas Baptist Clinic

🇺🇸

Jonesboro, Arkansas, United States

Englewood Hospital and Medical Center

🇺🇸

Englewood, New Jersey, United States

Hamilton Foot Care

🇺🇸

Baltimore, Maryland, United States

University of Florida

🇺🇸

Jacksonville, Florida, United States

Florida Research Network, LLC

🇺🇸

Gainesville, Florida, United States

UNC Hospitals University of North Carolina

🇺🇸

Chapel Hill, North Carolina, United States

Foot and Ankle Center of Illinois

🇺🇸

Springfield, Illinois, United States

Animas Foot and Ankle

🇺🇸

Gallup, New Mexico, United States

Office of Gerard J. Furst, DPM, PLLC

🇺🇸

East Setauket, New York, United States

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