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Search Allelic Imbalance of Expression of BRCA Genes in Hereditary Risk of Breast and/or Ovarian Cancer

Phase 2
Completed
Conditions
Hereditary Breast and Ovarian Cancer Syndrome
Interventions
Genetic: blood collection
Registration Number
NCT01333748
Lead Sponsor
Centre Francois Baclesse
Brief Summary

The purpose of this study is to determine proportion of patients presented a search allelic imbalance of expression of genes BRCA 1 and 2 in population with hereditary breast and/or ovarian cancer risk and negative for deletion mutation BRCA 1 and 2 genes

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
530
Inclusion Criteria

For patients

  • Women with breast cancer and / or ovarian cancer meet criteria suggestive of a hereditary predisposition
  • Deleterious mutation of BRCA1 and BRCA2 sought and not highlighted
  • Age ≥ 18 years
  • Agreeing to participate in the study (a collection of signed informed consent)

For control population

  • Women with no history of breast and / or ovarian cancer and no family history of breast and / or ovarian cancer among family members on the 1st and 2nd degree before age 50 for breast cancer and before 60 years for ovarian cancer
  • Agreeing to participate in the study (a collection of signed informed consent)
Exclusion Criteria

For patients:

  • Patients with a known deleterious mutation in BRCA1 and BRCA2
  • Patients do not meet criteria suggestive of a hereditary predisposition
  • Persons deprived of liberty or under guardianship (including guardianship)

For control population:

  • Males
  • Personal or family history of breast and / or ovarian cancer (breast or ovarian cancer in their family experienced 1st and 2nd degree before age 50 for breast cancer before age 60 for cancer ovarian)
  • Persons deprived of liberty or under guardianship (including guardianship)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
patients groupblood collectionPatients with ovarian and/or breast cancer
control populationblood collectioncontrol population without history of breast and/or ovarian cancer
Primary Outcome Measures
NameTimeMethod
estimate the proportion of patients with allelic imbalance at the level of expression of BRCA1blood sample at baseline, no follow-up in this study

The main objective of this study is to estimate the proportion of patients with allelic imbalance at the level of expression of BRCA1 in a population meeting the criteria suggestive of a hereditary predisposition to breast and / or ovarian cancer , and negative for deleterious mutations of BRCA 1 and BRCA 2.

Secondary Outcome Measures
NameTimeMethod
Study the variability of the measurement of the allelic expression depending on the position of SNPsblood sample at baseline, no follow-up in this study

Study the variability of the measurement of the allelic expression depending on the position of SNPs (Single Nucleotide Polymorphism) in the gene, in order to extend this research to variants of unknown significance whatever their position in the gene.

proportion of patients with allelic imbalance at the level of expression of the BRCA2 geneblood sample at baseline, no follow-up in this study

Estimate the proportion of patients with allelic imbalance at the level of expression of the BRCA2 gene in the same population.

Observe the possible effect of ageblood sample at baseline, no follow-up in this study

The frequency of allelic imbalance of expression will be compared depending on the age of the witnesses in the control population. The potential effect of age on the presence or absence of allelic imbalance of expression will be observed.

Trial Locations

Locations (5)

CHU

🇫🇷

Rouen, France

Dr Pascaline BERTHET

🇫🇷

Caen, France

Centre Hospitalier

🇫🇷

Cherbourg, France

Centre Eugène MARQUIS

🇫🇷

Rennes, France

Centre Henri BECQUEREL

🇫🇷

Rouen, France

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