The Comparison of Oxidative Stress Between Inhalation Anesthetics

Completed

• Conditions: Oxidative Stress; Cardiac Surgery

• Registration Number: NCT00965445
• Lead Sponsor: Chang Gung Memorial Hospital

Brief Summary

Oxidative stress is an unavoidable event during cardiac surgery. Isoprostanes have been demonstrated to be a reliable biomarker for the evaluation of oxidative stress in vivo.The aims of this study are(1)to develop an accurate liquid chromatography-tandem mass spectrometry methods for the detection of urinary isoprostane isomers in samples collected from healthy volunteers(for method quality control) and patients receiving a cardiac surgery (2)to investigate the change of isoprostanes after cardiopulmonary bypass(CPB) (3)to investigate the effect of different anesthetics on isoprostanes.

Detailed Description

Enhanced production of oxygen free radicals can lead to the generation of oxidative stress, which is harmful to human tissue and organs. Reactive oxygen species are released abruptly during some surgical procedures, and they are the major causes of ischemia-reperfusion injuries.Isoprostanes have been demonstrated to be a reliable biomarker for the evaluation of oxidative stress in vivo. Therefore, accurately monitoring and avoiding the occurrence of oxidative stress during surgery is an important clinical issue.

Isoprostanes are a series of prostaglandin-like compounds produced by non-enzymatic peroxidation of arachidonic acid.The aims of this study are(1)to develop an accurate liquid chromatography-tandem mass spectrometry methods for the detection of urinary isoprostane isomers in samples collected from patients receiving a cardiac surgery (2)to investigate the change of isoprostanes after cardiopulmonary bypass (3)to investigate the effect of different anesthetics on isoprostanes during surgery.

Study Timeline

• Study Start: 2007-03
• Primary Completion: 2007-12
• Study Completion: 2007-12
• Status Verified: 2009-08
• Study First Submitted: 2009-08-23
• Study First Submitted QC: 2009-08-23
• Last Update Submitted: 2009-08-24
• Study First Posted: 2009-08-25
• Last Update Posted: 2009-08-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

• patients receiving cardiac valve surgery in ASA II-III physical status

Exclusion Criteria

• trauma, infection, low ejection fraction (less than 25%), liver cirrhosis, or acute renal failure, and emergency surgery

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
isoprostanes isomer as marker of oxidative stress	one day

Trial Locations

Locations (1)

Dept. of Anesthesiology, Chang Gung Memorial Hospital; 🇨🇳 Taipei, Taiwan