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A Phase III Clinical Trial to Determine the Safety and Efficacy of ZF2001 for Prevention of COVID-19

Phase 3
Conditions
Safety and Efficacy
Prevention of COVID-19
Interventions
Biological: Recombinant new coronavirus vaccine (CHO cell) group
Biological: Recombinant new coronavirus vaccine (CHO cells) placebo group
Registration Number
NCT04646590
Lead Sponsor
Anhui Zhifei Longcom Biologic Pharmacy Co., Ltd.
Brief Summary

A randomized, double-blind, placebo-controlled international multicenter clinical trial design will be adopted. A total of 29,000 subjects aged 18 years and above are planned to be recruited, including 750 subjects aged 18-59 years and 250 subjects aged 60 years and above in China; 21,000 subjects aged 18-59 years and 7,000 subjects aged 60 years and above will be recruited outside China. Safety and immunogenicity will be evaluated among the Chinese subjects, and efficacy, immunogenicity and safety will be evaluated among the subjects outside China. Among them, 750 subjects aged 18-59 and 250 subjects aged 60 and above from outside China and all subjects from China will be selected as the immunogenicity subgroup for immunogenicity bridging study. The IgG levels of SARS-COV-2 neutralizing antibody and RBD protein binding antibody will be detected by blood sampling before vaccination, 14 days and 6 months after full course of vaccination to evaluate the immunogenicity and immune persistence.

Detailed Description

After signing the informed consent form, the volunteers aged 18 years and above will receive the relevant examinations after an inquiry by investigator of the medical history (including COVID-19 history), recent medication(vaccine) history, allergy history and concomitant medications, and demographic data collection by the investigators, including physical examination (skin and mucous membranes, lymph nodes, head, neck, chest, abdomen, spine/limbs), novel Coronavirus (SARS-COV-2) nucleic acid test and antibody test, urine pregnancy (women of childbearing age) test, and vital signs (blood pressure, axillary/oral temperature, pulse) evaluation.

Screening eligible subjects will be 1:1 randomly assigned to the experimental group and the placebo control group, and vaccinated as per the 0, 1, 2 month immunization schedule.

All adverse events (AEs) up to 30 minutes after each dose of vaccination, all AEs from 0 to 7 days (including both solicited and unsolicited), and all AES from 8 to 30 days (unsolicited) will be collected; All serious adverse events (SAEs) will be collected from the first dose of vaccination to 12 months after the whole vaccination.

The incidence rate and efficacy of any severity of COVID-9 of any severity 14 days after whole vaccination.

Blood samples (5ml) will be collected before the first dose of vaccination and 14 days and 6 months after the whole course of vaccination to detect neutralizing antibody of SARS-COV-2 and protein binding antibody IgG of receptor binding region (RBD) .

Recruitment & Eligibility

Status
UNKNOWN
Sex
All
Target Recruitment
28904
Inclusion Criteria
  • Population aged 18 years and above;
  • Subjects voluntarily participate in the study and sign the informed consent form; and are able to provide valid identification, and understand and comply with the requirements of the trial protocol;
  • Female subjects of childbearing age agree to use effective contraceptive measures from the beginning of the study to 2 months after full course of vaccination.
Exclusion Criteria
  • Suspected or confirmed as fever(axillary temperature ≥37.3°C / oral temperature ≥37.5°C) within 72 hours before the enrollment, or axillary temperature ≥37.3°C / oral temperature ≥37.5°C at the day of screening;

  • Diastolic blood pressure ≥ 100mmhg and / or systolic blood pressure ≥ 150mmhg;

  • Patients with previous history of a COVID-19;

  • Detection of SARS-COV-2 nucleic acid or antibody is positive;

  • Those who are suffering from the following diseases:

    1. With thrombocytopenia, any coagulation dysfunction or receiving anti-coagulatory treatment
    2. Congenital or acquired immune deficiency or autoimmune disease history; no spleen, or history of splenic surgery and trauma, or receiving immunomodulator treatment within 6 months, e.g., immunosuppressive dose of glucocorticoids (reference dose: equivalent to 20mg/ day of prednisone, over 1 week); Or monoclonal antibodies; Or thymosin; Or interferon etc.; However, topical application (such as ointment, eye drops, inhalers or nasal sprays) is permitted;
    3. Symptoms related to acute respiratory tract infection (such as sneezing, nasal congestion, runny nose, cough, sore throat, loss of taste, chills, shortness of breath, etc.);
    4. Cancer patients (except basal cell carcinoma).
  • With a history of serious allergy to any vaccine or any composition of Investigational product (including: aluminum preparations), such as allergic shock, allergic throat edema, allergic purpura, thrombocytopenic purpura, localized allergic necrosis reaction (Arthus reaction), dyspnea and angioneuroedema;

  • Inoculated with subunit vaccine and inactivated vaccine within 14 days before the first dosing of investigational vaccine, or inoculated with attenuated live vaccine within 30 days;

  • Previous receiving blood transfusion or blood relevant products (including immunoglobulin) within 3 months, or planning to receive such products from the starting of study to <6 months after the whole-course inoculation;

  • Have participated in or are participating in other covid-19 related clinical trials;

  • Women in breastfeeding period or in pregnant period (including women at childbearing age with positive result of urine pregnancy test);

  • Considered by investigators as any disease or state possibly making the subject at unacceptable risk; not conforming to the requirements of study protocol; interference of assessment of reactions of vaccine.

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Investigational VaccineRecombinant new coronavirus vaccine (CHO cell) groupRecombinant Novel Coronavirus Vaccine (CHO cell), 0.5mL/vial, contains 25 μg NCP-RBD protein. After mixing, take all the liquid and inject intramuscularly into deltoid muscle of upper arm.
Placebo comparatorRecombinant new coronavirus vaccine (CHO cells) placebo groupPlacebo for Recombinant Novel Coronavirus Vaccine (CHO cell), 0.5mL/vial, contains 0.25mg Aluminum hydroxide adjuvant. After mixing, take all the liquid and inject intramuscularly into deltoid muscle of upper arm.
Primary Outcome Measures
NameTimeMethod
The endpoint of safety studyFrom the first dose of vaccination until 12 months after full course of vaccination

Analysis of serious adverse events from the first dose of vaccination until 12 months after full course of vaccination

The endpoint of efficacy study14 days to one year after whole vaccination

The number of any severity of COVID-9 cases 14 days after whole vaccination

Secondary Outcome Measures
NameTimeMethod
The endpoint of efficacy studyUp to one year after the vaccination

The number of severe and severity above COVID-19 cases 14 days after whole vaccination; The number of any severity of COVID-9 cases after first dose of vaccination; The number of COVID-19 cases of any severity in populations of different age group (18-59 years vs. 60 years and above) 14 days after whole vaccination.

Endpoint of immunogenicity and immune persistence studyAt 14 days and 6 months after full course of vaccination

The level of neutralizing antibody to SARS-COV-2 and IgG level of RBD protein binding antibody at 14 days and 6 months after full course of vaccination.

Trial Locations

Locations (18)

GUVD Poliklinika

🇺🇿

Tashkent, Uzbekistan

Hunan Provincial Center for Disease Control and Prevention

🇨🇳

Changsha, Hunan, China

Fkui-Rscm

🇮🇩

Jakarta, Daerah Khusus Ibukota Jakarta, Indonesia

Aziz Fatimah Hospital

🇵🇰

Faisalabad, Punjab, Pakistan

National Hospital & Medical Center

🇵🇰

Lahore, Punjab, Pakistan

Central Park Teaching Hospital

🇵🇰

Lahore, Punjab, Pakistan

Indus Hospital and Health Network

🇵🇰

Karachi, Sindh, Pakistan

University of Health Sciences

🇵🇰

Lahore, Punjab, Pakistan

Al-Shifa Trust Eye Hospital

🇵🇰

Rawalpindi, Punjab, Pakistan

IIV Markaziy Poliklinika

🇺🇿

Tashkent, Uzbekistan

Toshkent viloyat IIV Poliklinika

🇺🇿

Tashkent, Uzbekistan

Shaheed Zulfiqar Ali Bhutto Medical University

🇵🇰

Islamabad, Pakistan

Biodimed Guayaquil Clinical Research Center

🇪🇨

Guayaquil, Guayas, Ecuador

Family Hospital No.15

🇺🇿

Tashkent, Uzbekistan

Biodimed Alemania Clinical Research Center

🇪🇨

Quito, Pichincha, Ecuador

Biodimed Eloy Alfaro Clinical Research Center

🇪🇨

Quito, Pichincha, Ecuador

FK Unpad/RSHS

🇮🇩

Bandung, Jawa Barat, Indonesia

Avicenna Hospital

🇵🇰

Lahore, Punjab, Pakistan

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