Apolipoprotein (APO)E Genotype, Meal Fatty Acids, Postprandial Lipaemia

Not Applicable

Completed

• Conditions: Cardiovascular Disease
• Interventions: Dietary Supplement: High unsaturated fat meal; Dietary Supplement: High saturated fat meal; Dietary Supplement: Saturated fatty acids and fish oil meal

• Registration Number: NCT01522482
• Lead Sponsor: University of Reading

Brief Summary

Cardiovascular disease (CVD) is the greatest cause of morbidity and mortality in the UK. Abnormalities in the concentration and/or composition of lipoproteins (the lipid carrying particles), in particular low density lipoproteins (LDL) in circulation, is one of the most important physiological defects contributing to the development of CVD.

The LDL cholesterol (LDLC) response to fatty acid change is in part mediated by the APOE genotype, with E4 individuals (25% of the UK population) being most responsive to changes in dietary fats, showing greater reductions when low levels of saturated fats or fish oils are consumed and greater increases when high levels of these fats are consumed. Therefore the aims of the present study is to understand the mechanism that regulates the higher LDLC response associated with saturated fatty acids and fish oil consumption in healthy men prospectively recruited based on their APOE genotype.

Detailed Description

Not available

Study Timeline

• Study Start: 2009-03
• Primary Completion: 2009-12
• Study Completion: 2011-07
• Status Verified: 2012-01
• Study First Submitted: 2012-01-26
• Study First Submitted QC: 2012-01-30
• Study First Posted: 2012-01-31
• Last Update Submitted: 2012-01-31
• Last Update Posted: 2012-02-02

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 31

Inclusion Criteria

• Gender Male
• Age 18-70 years
• Body Mass Index (BMI) < 32 kg/m2
• Plasma triglycerides 1-4 mmol/l
• Plasma cholesterol < 8 mmol/l
• Glucose < 7 mmol/l
• Haemoglobin > 11 g/dl
• ApoE E3/E3, E3/E4

Exclusion Criteria

• Blood pressure > 200/95 mmHg
• Had suffered a myocardial infraction or stroke in previous 2 years.
• Diabetes mellitus
• Liver disease
• Other endocrine disorders
• Unstable angina
• Familial hyperlipidaemia
• Any dietary restrictions or an a weight reducing diet
• On fatty acid supplements e.g. evening primrose oil or fish oils
• Vigorous exercise e.g. competitive athletes
• ApoE2/E2, apoE2/E3 and apoE2/E4
• Any other parameter on which the investigators felt an individual was unsuitable

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
High unsaturated fat meal	High unsaturated fat meal	Provided a fatty acid profile representative of a typical UK diet
High saturated fat meal	High saturated fat meal	-
Saturated fatty acid and fish oils meal	Saturated fatty acids and fish oil meal	Equivalent to two portions of oily fish

Primary Outcome Measures

Name	Time	Method
Impact of APOE genotype and dietary fat composition in plasma lipids	3 months

Secondary Outcome Measures

Name	Time	Method
Cardiovascular disease risk factors	3 months

Trial Locations

Locations (1)

Department of Food and Nutritional Sciences, University of Reading; 🇬🇧 Reading, United Kingdom