Periodontal Inflammation and Tryptophan-Kynurenine Metabolism in Parkinson's Disease
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 60
- 试验地点
- 1
- 主要终点
- Pocket probing depth
研究概览
简要总结
Parkinson's disease is a slowly progressing disease that affects the motor and non-motor functions of an individual. Periodontitis is an inflammatory chronic disease resulting in the destruction of tooth supporting structures. Inflammation has been identified as an important factor in both diseases. Given this common mechanism, the question arises as to how they may affect each other when both diseases occur together. In this study, we compared the metabolism of tryptophan, a natural amino acid of the human body, and observed whether such a process provides a biological link between periodontitis and Parkinson's disease. We extracted the saliva and blood samples of patients of Parkinson's disease and periodontitis, patients of periodontitis and healthy human beings. Comparisons between the three were made, and it was found that levels of some metabolites of tryptophan were higher in the patients of both diseases. These findings raise the possibility of a close biological connection between periodontal and brain health. Through an increased understanding of this connection in the future, new approaches for diagnosis and treatment of Parkinson's disease and periodontitis may be developed.
详细描述
Saliva and serum samples were collected from 20 Stage III, Grade B periodontitis patients with PAD (Parkinson+periodontitis group) and without PAD (periodontitis group), and 20 periodontally and systemically healthy (control group). Samples were analyzed for TRP, KYN, KYN/TRP ratio, KYNA, 3OHKYN, picolinic acid (PA), and quinolinic acid (QA) by liquid chromatography-mass spectrometry. Clinical periodontal parameters [plaque index (PI), probing pocket depth (PPD), clinical attachment loss (CAL), and bleeding on probing (BOP)] were recorded.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 75 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •systemically healthy
- •clinical diagnosis of periodontitis
- •clinical diagnosis of periodontal health
排除标准
- •history of regular use of systemic antibiotics anti-inflammatory, or antioxidant drugs (previous 3 months)
- •nonsurgical periodontal treatment (previous 6 months)
- •surgical periodontal treatment (previous 12 months)
- •presence of<10 teeth
- •current medications affecting gingival health (calcium channel blockers, phenytoin, cyclosporine, and hormone replacement therapy)
- •diagnosis of rheumatoid arthritis
- •pregnancy
- •lactating
- •excessive alcohol consumption.
结局指标
主要结局
Pocket probing depth
时间窗: 6 months
Measurement of the depth of a sulcus or periodontal pocket determined by measuring distance from a gingival margin to the base of the sulcus or pocket with a calibrated periodontal probe.
Clinical attachment level
时间窗: 6 months
Clinical attachment level (or loss, CAL) is a more accurate indicator of the periodontal support around a tooth than probing depth alone. CAL is measured from a fixed point on the tooth that does not change, the CEJ. To calculate CAL, two measurements are needed: distance from the gingival margin to the CEJ and probing depth. In recession: probing depth (+) gingival margin to the CEJ (add). In tissue overgrowth: probing depth (-) gingival margin to the CEJ (subtract)
Bleeding on probing
时间窗: 6 months
referring to bleeding that is induced by gentle manipulation of the tissue at the depth of the gingival sulcus, or interface between the gingiva and a tooth.
次要结局
- Saliva and serum samples processing and analyses(1 month)
研究者
Melis Yilmaz
Assistant Prof Dr
Medipol University