Complement Regulatory Proteins Expression and Clinical Response in Rheumatoid Arthritis (RA) Patients Treated With Rituximab

• Conditions: Rheumatoid Arthritis

• Registration Number: NCT01096069
• Lead Sponsor: Hospital de Clinicas de Porto Alegre

Brief Summary

There is a correlation between the CD55, CD59, CD35 and CD46 expression on B lymphocytes of patients before and after treatment with rituximab and the level of depletion and repopulation time for these cells. The theoretical rationale of the study assumes that the correlation, if any, will be a negative correlation. However, the hypothesis of positive correlation (two-tailed test) will also be tested.

Detailed Description

Rituximab, an anti-CD20 monoclonal antibody, is a new alternative treatment for patients with severe immune diseases and resistance to conventional treatment. One of the mechanisms of action of this drug is the complement-mediated lysis. Most RA patients respond well to rituximab treatment, however, some are refractory and mechanism of this non-response is still unclear. The role of CD55 and CD59 protein and its overexpression as a mechanism of resistance to rituximab treatment in lymphoma patients have been investigated. However, there has not been studied a correlation between the intensity of expression of these regulatory molecules on B cells of RA patients and resistance to treatment with Rituximab.

Study Timeline

• Study First Submitted: 2010-02-23
• Status Verified: 2010-03
• Study First Submitted QC: 2010-03-29
• Last Update Submitted: 2010-03-29
• Study First Posted: 2010-03-30
• Last Update Posted: 2010-03-30
• Study Start: 2010-06
• Primary Completion: 2010-12
• Study Completion: 2011-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

1. Patients of both gender, aged greater than or equal to 18 years; and lower than 70 years.
2. Patients diagnosed with RA for at least 6 months according to the criteria of the American College of Rheumatology (ACR) in 1987 for classification of AR.
3. Patients with a medical indication for therapy with rituximab, with prescription of this therapy for the first time and have not yet received the first dose.
4. DAS28 score greater than or equal to 3.2.
5. Use of adequate contraception, as judged from the attending physician.
6. Desire to participate voluntarily, the ability to understand the protocol, documented by signing the consent form (attached).

Exclusion Criteria

1. overlapping rheumatic disease, autoimmune lymphoproliferative and neoplastic.
2. active infection, patients with the use of cytotoxic drugs, presence of positive serology for HCV, HBV and HIV, active or latent tuberculosis.
3. allergy or hypersensitivity to rituximab.
4. pregnant woman or performing breastfeeding.
5. participating in another clinical study with intervention.
6. with functional class IV defined based on the criteria of Steinbrocker functional classification for RA.
7. prior therapy with Rituximab.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified