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HO-1/CREB3 Modulates Golgi Stress in Patients With Sepsis

Recruiting
Conditions
Sepsis
Interventions
Other: nothing
Registration Number
NCT06045780
Lead Sponsor
Tianjin Nankai Hospital
Brief Summary

This study was an ambispective observational cohort study that included sepsis patients hospitalized in the Intensive care unit (ICU) of the Nankai Hospital. All patients met the consensus criteria of sepsis-3. The purpose of this study is to investigate the valuable biomarkers for the severity of sepsis and predictors of 30-day mortality for patients with sepsis.

Detailed Description

All patients met the consensus criteria of sepsis-3. The purpose of this study is to investigate the valuable biomarkers for the severity of sepsis and predictors of 30-day mortality for patients with sepsis.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
150
Inclusion Criteria
  1. Males or females over the age of 18;
  2. Sepsis was diagnosed within 48h which meets SPESIS 3 criteria;
  3. Capable of understanding the purpose and risk of the study;
  4. Patients or proxy must give written informed consent before any assessment is performed.
Exclusion Criteria
  1. Pregnancy, lactation or perinatal period;
  2. Breast carcinoma;
  3. HIV seropositive or Syphilis seropositive;
  4. Any clinical-relevant condition that might affect study participation and/or study results;
  5. Participation in any other intervention trial;
  6. Unwillingness or inability to following the study protocol in the investigators opinion.

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Arm && Interventions
GroupInterventionDescription
Healthy controlsnothinghealthy
Sepsisnothingdisease
Primary Outcome Measures
NameTimeMethod
cAMP-response element binding protein 3, CREB3Up to Day 28

CREB3 levels were measured in the PBMCs of sepsis patients and healthy controls

ADP-ribosylation factor 4, ARF4Up to Day 28

ARF4 levels were measured in the PBMCs of sepsis patients and healthy controls

Secondary Outcome Measures
NameTimeMethod
Heme oxygnase-1, HO-1Up to Day 28

HO-1 levels were measured in the PBMCs of sepsis patients and healthy controls

Oxygenation indexUp to Day 28

The ratio of arterial oxygen partial pressure (PaO2 in mmHg) to fractional inspired oxygen (FiO2 expressed as a fraction, not a percentage).

Mechanical ventilation daysUp to Day 28

The number of days the patient was alive and mechanical ventilating

Sequential Organ-Failure Assessment (SOFA) scoreUp to Day 28

The SOFA score is used to assess the severity and prognosis of sepsis. SOFA was based on six different scores, one for each of the respiratory, cardiovascular, hepatic, coagulation, renal and neurological systems each scored from 0 to 4 with an increasing score reflecting worsening organ dysfunction.

ARF familyUp to Day 28

ARF1, ARF3, ARF5, ARF6 levels were measured in the PBMCs of sepsis patients and healthy controls

Inflammation biomarkers Inflammation levelsUp to Day 28

Procalcitonin (PCT), C-reactive protein (CRP), tumor necrosis factor-alpha (TNF-alpha), interleukin-1 beta (IL-1β), and interleukin-6 (IL-6)

ICU stay lengthUp to Day 90

The number of days the patient was alive and ICU stay

28 days surviveUp to Day 28

All Cause Mortality to Day 28

Acute Physiology and Chronic Health Evaluation II (APACHE II) scoreUp to Day 28

The APACHE II score consists of three parts: acute physiological score, age score, and chronic health score. APACHE II uses a point score (range 0 to 71) based upon initial values of 12 routine physiologic measurements, age, and previous health status to provide a general measure of severity of disease, with an increasing score reflecting worsening organ dysfunction.

Trial Locations

Locations (1)

Tianjin Nankai Hospital

🇨🇳

Tianjin, Tianjin, China

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