Effects of Sleep Restriction on BAT Activation in Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 4
- 试验地点
- 1
- 主要终点
- Change from baseline Brown adipose tissue (BAT) in 6 weeks
研究概览
简要总结
The goal of this proposed research is to test the hypothesis that long-term mild sleep restriction (SR), as occurs frequently in adults and adolescents, leads to a positive energy balance and weight gain.
Aim 1. To determine the effects of SR, relative to habitual sleep (HS), on food choice and energy intake (EI) in adults at risk of obesity.
- Hypothesis 1a. EI, assessed by multiple weekly 24-hour recalls, will be greater during a period of SR relative to HS. This will be mostly due to increased fat and carbohydrate intakes.
- Hypothesis 1b. Neuronal responses to food stimuli, assessed by functional MRI (fMRI) after 6 weeks of SR or HS, will indicate increased activity in networks associated with reward and food valuation (insula, orbitofrontal cortex) during a period of SR relative to HS. These responses will be correlated with intakes of high carbohydrate and high fat foods (hypothesis 1a) and neuropeptide Y (NPY). Moreover, activation of the default mode network (DMN) will be suppressed to a lesser extent after SR compared to HS.
Aim 2. To determine the effects of SR, relative to HS, on energy expenditure (EE) via independent and complementary approaches.
- Hypothesis 2a. EE, assessed by doubly-labeled water (DLW), and physical activity level, monitored daily by actigraphy, will be lower during SR relative to HS.
- Hypothesis 2b. Brown adipose tissue (BAT), assessed by positron emission tomography and magnetic resonance combined scanner (PET/MR) using 18F-fluorodeoxyglucose (18FDG-PET) and fat fraction (FF) measurement under cold stimulation, will be greater after SR relative to HS. This would suggest higher adaptive thermogenesis after SR compared to HS. BAT activation will also be correlated with NPY.
Aim 3. To determine whether SR alters body weight and adiposity relative to HS.
- Hypothesis 3a. SR will lead to weight gain and increased total adiposity, as assessed using magnetic resonance imaging (MRI), relative to HS.
- Hypothesis 3b. Increased adiposity after SR will be correlated to an adverse cardio-metabolic risk profile (increased glucose, insulin, triglycerides, leptin, reduced high-density lipoprotein cholesterol and adiponectin) and neuronal responses to food stimuli (Hypothesis 1b), and EE (Hypothesis 2a & 2b). Failure to stimulate BAT with SR will be associated with greater gain in adiposity.
详细描述
There is an association between short sleep duration (SSD) and obesity. Moreover, short sleepers (<7 hours sleep/night) gain more weight over time than normal sleepers (7-8 hours sleep/night). These relationships are increasingly supported by clinical data showing that restricting sleep duration in healthy, normal weight adults, increases energy intake (EI).
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 20 Years 至 49 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Normal scores on:
- •Pittsburgh Quality of Sleep Questionnaire
- •Epworth Sleepiness Scale
- •Berlin Questionnaire
- •Sleep Disorders Inventory Questionnaire
- •Beck Depression Inventory
- •Composite Scale of Morningness/Eveningness
- •Three Factor Eating Questionnaire
- •Sleep 7-9 hours in bed/night with no daytime nap
- •Age 20-49 years, premenopausal women
- •All racial/ethnic groups
- •Body mass index 25-29.9 kg/m2
排除标准
- •Smokers (any cigarettes or ex-smoker <3 years)
- •Neurological, medical or psychiatric disorder, diabetics
- •Eating and/or sleep disorders
- •Contraindications for MRI scanning
- •Travel across time zones within 4 weeks
- •History of drug and alcohol abuse
- •Shift worker (or rotating shift worker)
- •Caffeine intake >300 mg/d
- •Pregnancy or within 1 y post-partum
- •Heavy equipment operators Commercial long-distance drivers
研究组 & 干预措施
TSD-PSR
Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Habitual Sleep (Behavioral)
PSR-TSD
Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Partial Sleep Restriction (Behavioral)
PSR-TSD
Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Total Sleep Deprivation (Behavioral)
PSR-TSD
Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Habitual Sleep (Behavioral)
TSD-PSR
Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Partial Sleep Restriction (Behavioral)
TSD-PSR
Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
干预措施: Total Sleep Deprivation (Behavioral)
结局指标
主要结局
Change from baseline Brown adipose tissue (BAT) in 6 weeks
时间窗: 6 weeks
Assessed by positron emission tomography and magnetic resonance combined scanner (PET/MR) using 18F-fluorodeoxyglucose (18FDG-PET). Fat fraction (FF) measurement under cold stimulation is to be measured.
次要结局
- Difference in Glucose level from baseline at 6 weeks(6 weeks)
- Difference in weight from baseline at 6 weeks(6 weeks)
研究者
Marie-Pierre St-Onge
Assistant Professor of Nutritional Medicine, Dept of Medicine Endocrinology
Columbia University