Effects of Sleep Restriction on BAT Activation in Humans

Not Applicable

Completed

• Conditions: Sleep Deprivation; Obesity
• Interventions: Behavioral: Partial Sleep Restriction; Behavioral: Total Sleep Deprivation; Behavioral: Habitual Sleep

• Registration Number: NCT02770118
• Lead Sponsor: Columbia University

Brief Summary

The goal of this proposed research is to test the hypothesis that long-term mild sleep restriction (SR), as occurs frequently in adults and adolescents, leads to a positive energy balance and weight gain.

Aim 1. To determine the effects of SR, relative to habitual sleep (HS), on food choice and energy intake (EI) in adults at risk of obesity.

* Hypothesis 1a. EI, assessed by multiple weekly 24-hour recalls, will be greater during a period of SR relative to HS. This will be mostly due to increased fat and carbohydrate intakes.

* Hypothesis 1b. Neuronal responses to food stimuli, assessed by functional MRI (fMRI) after 6 weeks of SR or HS, will indicate increased activity in networks associated with reward and food valuation (insula, orbitofrontal cortex) during a period of SR relative to HS. These responses will be correlated with intakes of high carbohydrate and high fat foods (hypothesis 1a) and neuropeptide Y (NPY). Moreover, activation of the default mode network (DMN) will be suppressed to a lesser extent after SR compared to HS.

Aim 2. To determine the effects of SR, relative to HS, on energy expenditure (EE) via independent and complementary approaches.

* Hypothesis 2a. EE, assessed by doubly-labeled water (DLW), and physical activity level, monitored daily by actigraphy, will be lower during SR relative to HS.

* Hypothesis 2b. Brown adipose tissue (BAT), assessed by positron emission tomography and magnetic resonance combined scanner (PET/MR) using 18F-fluorodeoxyglucose (18FDG-PET) and fat fraction (FF) measurement under cold stimulation, will be greater after SR relative to HS. This would suggest higher adaptive thermogenesis after SR compared to HS. BAT activation will also be correlated with NPY.

Aim 3. To determine whether SR alters body weight and adiposity relative to HS.

* Hypothesis 3a. SR will lead to weight gain and increased total adiposity, as assessed using magnetic resonance imaging (MRI), relative to HS.

* Hypothesis 3b. Increased adiposity after SR will be correlated to an adverse cardio-metabolic risk profile (increased glucose, insulin, triglycerides, leptin, reduced high-density lipoprotein cholesterol and adiponectin) and neuronal responses to food stimuli (Hypothesis 1b), and EE (Hypothesis 2a \& 2b). Failure to stimulate BAT with SR will be associated with greater gain in adiposity.

Detailed Description

There is an association between short sleep duration (SSD) and obesity. Moreover, short sleepers (\<7 hours sleep/night) gain more weight over time than normal sleepers (7-8 hours sleep/night). These relationships are increasingly supported by clinical data showing that restricting sleep duration in healthy, normal weight adults, increases energy intake (EI).

Study Timeline

• Study Start: 2016-01
• Study First Submitted: 2016-02-10
• Study First Submitted QC: 2016-05-10
• Study First Posted: 2016-05-12
• Primary Completion: 2017-06
• Status Verified: 2017-07
• Study Completion: 2017-07
• Last Update Submitted: 2017-07-17
• Last Update Posted: 2017-07-19

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 4

Inclusion Criteria

• Normal scores on:

  • Pittsburgh Quality of Sleep Questionnaire
  • Epworth Sleepiness Scale
  • Berlin Questionnaire
  • Sleep Disorders Inventory Questionnaire
  • Beck Depression Inventory
  • Composite Scale of Morningness/Eveningness
  • Three Factor Eating Questionnaire

• Sleep 7-9 hours in bed/night with no daytime nap

• Age 20-49 years, premenopausal women

• All racial/ethnic groups

• Body mass index 25-29.9 kg/m2

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Exclusion Criteria

• Smokers (any cigarettes or ex-smoker <3 years)
• Neurological, medical or psychiatric disorder, diabetics
• Eating and/or sleep disorders
• Contraindications for MRI scanning
• Travel across time zones within 4 weeks
• History of drug and alcohol abuse
• Shift worker (or rotating shift worker)
• Caffeine intake >300 mg/d
• Pregnancy or within 1 y post-partum
• Heavy equipment operators Commercial long-distance drivers

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
TSD-PSR	Partial Sleep Restriction	Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
PSR-TSD	Partial Sleep Restriction	Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
PSR-TSD	Habitual Sleep	Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
PSR-TSD	Total Sleep Deprivation	Partial sleep restriction (PSR) followed by total sleep deprivation (TSD). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
TSD-PSR	Total Sleep Deprivation	Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).
TSD-PSR	Habitual Sleep	Total sleep deprivation (TSD) followed by partial sleep restriction (PSR). Experimental procedures will begin with a 3-day period of habitual sleep (HS).

Primary Outcome Measures

Name	Time	Method
Change from baseline Brown adipose tissue (BAT) in 6 weeks	6 weeks	Assessed by positron emission tomography and magnetic resonance combined scanner (PET/MR) using 18F-fluorodeoxyglucose (18FDG-PET). Fat fraction (FF) measurement under cold stimulation is to be measured.

Secondary Outcome Measures

Name	Time	Method
Difference in Glucose level from baseline at 6 weeks	6 weeks	Assessed by fasting blood samples
Difference in weight from baseline at 6 weeks	6 weeks	Assessed by body composition and anthropometric measurements.

Trial Locations

Locations (1)

New York Nutrition Obesity Research Center; 🇺🇸 New York, New York, United States