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Fat Metabolism in Response to Acute Diet- and Exercise-induced Changes in Energy Balance

Not Applicable
Completed
Conditions
Cardiovascular Diseases
Registration Number
NCT00830999
Lead Sponsor
Washington University School of Medicine
Brief Summary

This study is being conducted to learn more about the role of diet and exercise in regulating plasma triglyceride (fat) metabolism. The investigators will examine the effect of acute (24 hour) changes in energy intake and expenditure on fat metabolism the following day.

Detailed Description

Excess body fat and a sedentary lifestyle are associated with increased plasma triglyceride (TG) and apolipoprotein B-100 (apoB-100) concentrations, which are important risk factors for the development of cardiovascular disease.

Weight loss and endurance exercise improve plasma lipid and lipoprotein concentrations. However, the mechanisms responsible for this effect are largely unknown, and much uncertainty remains regarding the independent roles of dietary energy intake, exercise energy expenditure, and net energy balance in controlling plasma TG concentrations.

The main goal of this project, therefore, is to investigate the mechanisms by which acute alterations in energy balance, induced by diet and/or physical activity (endurance exercise), regulate very-low density lipoprotein (VLDL) metabolism.

Subjects will be asked to perform 3 separate trials. One of these will always be an "energy balance" trial that will act as the control trial for the other 2 trials they perform. Subjects will therefore be randomized to 2 different study arms.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
12
Inclusion Criteria
  • overweight and obese men
  • normal to mild hypertriglyceridemia
Exclusion Criteria
  • Smoking
  • Any medical condition other than increased body weight (e.g. diabetes, heart disease, etc.).
  • Use of drugs known to affect lipid metabolism (e.g. statins, etc.).
  • Regular exercise training.

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Primary Outcome Measures
NameTimeMethod
Very Low Density Lipoprotein-Triglyceride and apoB-100 concentrationsAfter acute (24 hour) changes in energy intake and/or energy expenditure
Secondary Outcome Measures
NameTimeMethod
Liver Very Low Density Lipoprotein-Triglyceride and apoB-100 secretion ratesAfter acute (24 hour) changes in energy intake and/or energy expenditure
Plasma insulin concentrationDuring acute (24 hour) changes in energy intake and/or energy expenditure

Measured hourly over the 24 h period.

Plasma free fatty acid concentration and turnover rateAfter acute (24 hour) changes in energy intake and/or energy expenditure
Liver Very Low Density Lipoprotein-Triglyceride and apoB-100 clearance rates from plasmaAfter acute (24 hour) changes in energy intake and/or energy expenditure
Plasma glucose concentration and turnover rateAfter acute (24 hour) changes in energy intake and/or energy expenditure
Plasma glucose concentrationDuring acute (24 hour) changes in energy intake and/or energy expenditure

Measured hourly over the 24 h period.

Plasma free fatty acid concentrationDuring acute (24 hour) changes in energy intake and/or energy expenditure

Measured hourly over the 24 h period.

Trial Locations

Locations (1)

Washington University in Saint Louis

🇺🇸

Saint Louis, Missouri, United States

Washington University in Saint Louis
🇺🇸Saint Louis, Missouri, United States

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