MedPath

A Study to Evaluate the Efficacy, Safety and Tolerability of Mirabegron and Solifenacin Succinate Alone and in Combination for the Treatment of Overactive Bladder

Phase 2
Completed
Conditions
Urologic Diseases
Urinary Bladder Diseases
Signs and Symptoms
Urinary Bladder, Overactive
Urological Manifestations
Interventions
Registration Number
NCT01340027
Lead Sponsor
Astellas Pharma Europe B.V.
Brief Summary

The purpose of this study is to examine how well two medicines in combination (solifenacin succinate and mirabegron) work in the treatment of bladder problems over a 12-week period.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
1307
Inclusion Criteria
  • Inclusion Criteria at Visit 1/Screening:

    • Subject has a Body Mass Index (BMI) of between 18 and 35 kg/m^2 and a total body weight between 50 and 95 kg;
    • Subject is willing and able to complete the micturition diary and questionnaires correctly and is willing and able to measure his/her vital signs at home at stipulated time points, using the device provided by the study personnel, and to adequately record the readings;
    • Subject has symptoms of overactive bladder (OAB; urinary frequency, urgency and/or urgency incontinence) for at least 3 months.
  • Inclusion Criteria at Visit 3/Baseline:

    • Subject has experienced frequency of micturition on average ≥ 8 times per 24-hour period during the 3-day micturition diary period (incontinence episode should not be counted as a micturition);
    • Subject must experience at least 1 episode of urgency (grade 3 or 4) per 24-hour period (with or without urgency incontinence) during the 3 day micturition diary period.
Exclusion Criteria
  • Exclusion Criteria at Visit 1/Screening:

    • Subject is breastfeeding, pregnant or intends to become pregnant during the study. The pregnancy test (Beta Human Chorionic Gonadotropin in serum) at Screening must be negative in women of childbearing potential;
    • Female subjects of childbearing potential and not using a highly effective method of birth control during the study and for 30 days after final study drug administration.
    • Male subjects (unless surgically sterile) with female spouses/partners who are of childbearing potential, and not using a barrier method of contraception during the study and for 30 days after final study drug administration. In addition, female spouses/partners of male subjects and who are of childbearing potential should also use a highly effective method of birth control during the study and for 30 days after final study drug administration. Highly effective methods of birth control are defined as those, alone or in combination, that result in a low failure rate (i.e. less than 1% per year) when used consistently and correctly.
    • Subject has significant post-void residual (PVR) volume (> 150 mL);
    • Subject has significant stress incontinence or mixed stress/urgency incontinence where stress is the predominant factor as determined by the Investigator (for female subjects confirmed by the cough provocation test);
    • Subject has a neurological cause for detrusor overactivity;
    • Subject has an indwelling catheter or practices intermittent self-catheterization;
    • Subject has diabetic neuropathy;
    • Subject has chronic inflammation such as interstitial cystitis, bladder stones, previous pelvic radiation therapy or previous or current malignant disease of the pelvic organs;
    • Subject has had previous lower urinary tract or pelvic floor surgery (except cystoscopy);
    • Subject has had intravesical treatment in the past 12 months with e.g., botulinum toxin, resiniferatoxin, capsaicin;
    • Subject has uncontrolled narrow angle glaucoma, urinary or gastric retention, severe ulcerative colitis or Crohn's Disease, toxic megacolon, myasthenia gravis or any other condition which makes the use of anticholinergics contraindicated;
    • Subject has clinically significant cardiovascular or cerebrovascular diseases within 6 months prior to Screening, such as myocardial infarction, uncontrolled angina, significant ventricular arrhythmias, heart failure and stroke;
    • Subject is receiving current non-drug treatment including electro-stimulation therapy (with the exception of a bladder training program or pelvic floor exercises which started more than 30 days prior to Screening);
    • Subject is using medications intended to treat OAB or prohibited medications.
    • Subject has known or suspected hypersensitivity to solifenacin succinate, mirabegron or any of their excipients;
    • Subject has any significant neurological disease or defect affecting bladder function (e.g., neurogenic bladder, systemic or central neurological disease such as multiple sclerosis [MS] and Parkinson's disease);
    • Subject has severe hypertension which is defined as a sitting average systolic blood pressure ≥ 180 mmHg and/or an average diastolic blood pressure ≥ 110 mmHg;
  • Exclusion Criteria at Visit 2/Placebo Run-In:

    • Subject has evidence of a urinary tract infection (UTI) (urine culture containing > 100,000 cfu/mL). The subject can be enrolled into the study after successful treatment of the UTI (confirmed by a laboratory result of negative urine culture). However, the subject must be re screened if the initial screening visit was > 28 days;
    • Subject has a QT interval > 450 ms or is at risk of QT prolongation (e.g., family history of long QT syndrome, hypokalaemia) or is on drug treatment known to be associated with QT prolongation;
    • Subject has clinically significant abnormalities on the 12 lead electrocardiogram (ECG);
    • Subject has serum creatinine > 150 µmol/L, aspartate aminotransferase (AST) and/or alanine aminotransferase (ALT) > 2x upper limit of normal (ULN), gamma-glutamyltransferase (γ-GT) > 3x ULN, or total bilirubin > 2x ULN, as assessed in Screening samples;
  • Exclusion Criteria at Visit 3/Baseline:

    • Subject had an average total daily urine volume > 3000 mL as recorded in the micturition diary period;
    • Subject has severe hypertension which is defined as a sitting average systolic blood pressure ≥ 180 mmHg and/or an average diastolic blood pressure ≥ 110 mmHg.

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
PlaceboPlaceboParticipants received matching placebo tablets orally once a day for 12 weeks
Solifenacin 2.5 mgSolifenacin succinateParticipants received solifenacin 2.5 mg tablets orally once a day for 12 weeks
Solifenacin 10 mgSolifenacin succinateParticipants received solifenacin 10 mg tablets orally once a day for 12 weeks
Mirabegron 25 mgMirabegronParticipants received mirabegron 25 mg tablets orally once a day for 12 weeks
Mirabegron 50 mgMirabegronParticipants received mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 5 mgSolifenacin succinateParticipants received solifenacin 5 mg tablets orally once a day for 12 weeks
Solifenacin 2.5 mg and Mirabegron 25 mgMirabegronParticipants received solifenacin 2.5 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 2.5 mg and Mirabegron 50 mgMirabegronParticipants received solifenacin 2.5 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 2.5 mg and Mirabegron 25 mgSolifenacin succinateParticipants received solifenacin 2.5 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 2.5 mg and Mirabegron 50 mgSolifenacin succinateParticipants received solifenacin 2.5 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 5 mg and Mirabegron 25 mgMirabegronParticipants received solifenacin 5 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 5 mg and Mirabegron 25 mgSolifenacin succinateParticipants received solifenacin 5 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 5 mg and Mirabegron 50 mgMirabegronParticipants received solifenacin 5 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 5 mg and Mirabegron 50 mgSolifenacin succinateParticipants received solifenacin 5 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 10 mg and Mirabegron 25 mgMirabegronParticipants received solifenacin 10 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 10 mg and Mirabegron 25 mgSolifenacin succinateParticipants received solifenacin 10 mg and mirabegron 25 mg tablets orally once a day for 12 weeks
Solifenacin 10 mg and Mirabegron 50 mgMirabegronParticipants received solifenacin 10 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Solifenacin 10 mg and Mirabegron 50 mgSolifenacin succinateParticipants received solifenacin 10 mg and mirabegron 50 mg tablets orally once a day for 12 weeks
Primary Outcome Measures
NameTimeMethod
Change From Baseline to End of Treatment (EOT) in Mean Volume Voided Per MicturitionBaseline and Week 12

The average volume voided per micturition was calculated from the volume of each micturition measured by the participant and recorded in a micturition diary for 3 days before the Baseline and Week 12 clinic visits.

Secondary Outcome Measures
NameTimeMethod
Change From Baseline to Each Visit in Mean Volume Voided Per MicturitionBaseline and Weeks 2, 4, 8 and 12

The average volume voided per micturition was calculated from the volume of each micturition measured by the participant and recorded in a micturition diary for 3 days before the Baseline and each post-baseline clinic visit.

Change From Baseline to End of Treatment in Mean Number of Incontinence Episodes Per 24 HoursBaseline and Week 12

The average number of incontinence episodes (any involuntary leakage of urine) per day was derived from the number of incontinence episodes recorded by the participant in the micturition diary for 3-days before the Baseline and Week 12 clinic visits.

Change From Baseline to Each Visit in Mean Number of Micturitions Per 24 HoursBaseline and Weeks 2, 4, 8 and 12

The average number of micturitions (urinations) per 24 hours was derived from the number of urinations (excluding incontinence only episodes) per day recorded by the participant in the micturition diary for 3-days before the Baseline and each post-baseline clinic visit.

Percentage of Participants With Zero Incontinence Episodes Post-baselineWeeks 2, 4, 8 and 12

The percentage of participants with no incontinence episodes for the 3 days prior to each clinic visit derived from the micturition diary recorded by the participant.

Percentage of Participants With 50% Reduction in Incontinence EpisodesBaseline and Weeks 2, 4, 8 and 12

The percentage of participants with at least a 50% decrease from Baseline in mean number of incontinence episodes per 24 hours during the 3 days prior to each clinic visit derived from the participant's micturition diary.

Change From Baseline to Each Visit in Mean Number of Nocturia Episodes Per 24-HoursBaseline and Weeks 2, 4, 8 and 12

Nocturia is defined as waking at night one or more times to void. The average number of times a participant urinated (excluding incontinence only episodes) during sleeping time per day was derived from the 3-day micturition diary.

Change From Baseline to Each Visit in Mean Number of Urgency Episodes (Grade 3 and/or 4) Per 24 HoursBaseline and Weeks 2, 4, 8 and 12

The average number of urgency episodes (the sudden, compelling desire to pass urine, which is difficult to defer), derived from urgency episodes classified by the participant in the 3-day micturition diary as grade 3 or 4 on the Patient Perception of Intensity of Urgency Scale: 0: No urgency; 1: Mild urgency; 2: Moderate urgency, could delay voiding a short while; 3: Severe urgency, could not delay voiding; 4: Urge incontinence, leaked before arriving to the toilet.

Percentage of Participants With Deterioration in PPBCBaseline and Week 12

The PPBC scale is a global assessment tool that asks patients to rate their impression of their current bladder condition on a 6-point scale from 1: 'Does not cause me any problems at all'; 2: 'Causes me some very minor problems'; 3: 'Causes me some minor problems'; 4: 'Causes me (some) moderate problems'; 5: 'Causes me severe problems' and 6: 'Causes me many severe problems'. Deterioration was defined as at least a 1 point increase from Baseline in PPBC score.

Change From Baseline to End of Treatment in Symptom Bother Score as Assessed by the Overactive Bladder Questionnaire (OAB-q)Baseline and Week 12

Overactive bladder symptoms were assessed using the symptom bother scale of the overactive bladder questionnaire. The symptom bother scale consists of 8 questions answered by the participant on a scale from 1-6. The total symptom bother score was calculated from the 8 answers and then transformed to range from 0 to 100, with 100 indicating worst severity. A negative change from Baseline in symptom bother score indicates improvements.

Percentage of Participants With a Symptom Bother ResponseBaseline and Week 12

Overactive bladder symptoms were assessed using the symptom bother scale of the overactive bladder questionnaire. The symptom bother scale consists of 8 questions answered by the participant on a scale from 1-6. The total symptom bother score was calculated from the 8 answers and then transformed to range from 0 to 100, with 100 indicating worst severity. Symptom bother response is defined as improvement (decrease) of at least 10 points from Baseline.

Change From Baseline to End of Treatment in Health-related Quality of Life (HRQL) Total ScoreBaseline and Week 12

Health-related quality of life was assessed by the HRQL subscales (coping, concern, sleep and social interaction) of the overactive bladder questionnaire (OABq). The HRQL total score was calculated by adding the 4 HRQL subscale scores, and transforming to a scale from 0 to 100, with higher scores indicating better quality of life. A positive change from Baseline in HRQL score indicates improvements.

Change From Baseline to End of Treatment in European Quality of Life-5 Dimensions (EQ-5D) Visual Analog Scale (VAS)Baseline and Week 12

The EQ-5D is an international, standardized, generic instrument for describing and evaluating health status. Health status is assessed by patients evaluating their health on a vertical, visual analog scale from 0 to 100 where the endpoints are labeled 'Worst imaginable health state' (=0) and 'Best imaginable health state' (=100). On the EQ-5D VAS, a positive change from baseline indicates improvement.

Change From Baseline to End of Treatment in Mean Number of Micturitions Per 24 HoursBaseline and Week 12

The average number of micturitions (urinations) per 24 hours was derived from the number of urinations (excluding incontinence only episodes) per day recorded by the participant in the micturition diary for 3-days before the Baseline and Week 12 clinic visits.

Percentage of Participants With a Micturition ResponseBaseline and Weeks 2, 4, 8 and 12

A responder is defined as a participant with at most 8 micturitions per 24 hours post-baseline and a negative change (i.e. an improvement) from Baseline.

Change From Baseline to Each Visit in Mean Number of Incontinence Episodes Per 24 HoursBaseline and Weeks 2, 4, 8 and 12

The average number of incontinence episodes (any involuntary leakage of urine) per day was derived from the number of incontinence episodes recorded by the participant in the micturition diary for 3-days before the Baseline and each post-baseline clinic visit.

Change From Baseline to Each Visit in Mean Number of Urgency Incontinence Episodes Per 24 HoursBaseline and Weeks 2, 4, 8 and 12

Urgency incontinence is the involuntary leakage of urine accompanied by or immediately preceded by urgency, and was derived from the number of incontinence episodes classified by the participant in a 3-day micturition diary as Grade 3 or 4 on the Patient Perception of Intensity of Urgency Scale: 0 = No urgency; 1 = Mild urgency; 2 = Moderate urgency, could postpone voiding a short while; 3 = Severe urgency, could not postpone voiding; 4 = Urge incontinence, leaked before arriving to the toilet.

Percentage of Participants With a Health-related Quality of Life Total Score ResponseBaseline and Week 12

Health-related quality of life was assessed by the HRQL subscales (coping, concern, sleep and social interaction) of the overactive bladder questionnaire (OABq). The HRQL total score was calculated by adding the 4 HRQL subscale scores, and transforming to a scale from 0 to 100, with higher scores indicating better quality of life. HRQL response is defined as improvement (decrease) of at least 10 points from Baseline.

Change From Baseline in European Quality of Life-5 Dimensions (EQ-5D) Mobility ScoreBaseline and Week 12

The EQ-5D is an international, standardized, nondisease-specific (i.e., generic) instrument for describing and valuing health status. Participants were asked to indicate which of the following statements best describes their health state:

I have no problems in walking about; I have some problems in walking about; I am confined to bed.

In the table below, each row title lists Baseline health status first followed by End of Treatment health status and reports the number of patients in that category.

Change From Baseline to Each Visit in Mean Level of UrgencyBaseline and Weeks 2, 4, 8 and 12

Average of participants' ratings on the degree of urgency associated with each micturition and/or incontinence episode recorded in the 3-day micturition diary according to the Patient Perception of Intensity of Urgency Scale: 0: No urgency; 1: Mild urgency; 2: Moderate urgency, could delay voiding a short while; 3: Severe urgency, could not delay voiding; 4: Urge incontinence, leaked before arriving to the toilet.

Change From Baseline to Each Visit in Mean Number of Pads Used Per 24 HoursBaseline and Weeks 2, 4, 8 and 12

The average number of times a participant recorded a new pad used per day during the 3-day micturition diary period.

Change From Baseline to End of Treatment in Patient Perception of Bladder Condition (PPBC)Baseline and Week 12

The PPBC scale is a global assessment tool that asks patients to rate their impression of their current bladder condition on a 6-point scale from 1: 'Does not cause me any problems at all'; 2: 'Causes me some very minor problems'; 3: 'Causes me some minor problems'; 4: 'Causes me (some) moderate problems'; 5: 'Causes me severe problems' and 6: 'Causes me many severe problems'. A negative change from Baseline score indicates improvement.

Percentage of Participants With Improvement in PPBCBaseline and Week 12

The PPBC scale is a global assessment tool that asks patients to rate their impression of their current bladder condition on a 6-point scale from 1: 'Does not cause me any problems at all'; 2: 'Causes me some very minor problems'; 3: 'Causes me some minor problems'; 4: 'Causes me (some) moderate problems'; 5: 'Causes me severe problems' and 6: 'Causes me many severe problems'. Improvement was defined as at least a 1-point improvement (decrease) from Baseline in PPBC score.

Change From Baseline to End of Treatment in Treatment Satisfaction on Visual Analog Scale (TS-VAS)Baseline and Week 12

The TS-VAS is a visual analog scale (VAS) that asks patients to rate their satisfaction with treatment by placing a vertical mark on a 10 cm line where the endpoints are labeled 'No, not at all' on the left (=0) to 'Yes, completely satisfied' on the right (=10). A positive change from Baseline indicates improvement.

Percentage of Participants With Major Improvement in PPBCBaseline and Week 12

The PPBC scale is a global assessment tool that asks patients to rate their impression of their current bladder condition on a 6-point scale from 1: 'Does not cause me any problems at all'; 2: 'Causes me some very minor problems'; 3: 'Causes me some minor problems'; 4: 'Causes me (some) moderate problems'; 5: 'Causes me severe problems' and 6: 'Causes me many severe problems'. Major improvement was defined as at least a 2-point improvement (decrease) from Baseline in PPBC score.

Change From Baseline in European Quality of Life-5 Dimensions (EQ-5D) Self-care ScoreBaseline and Week 12

The EQ-5D is an international, standardized, nondisease-specific (i.e., generic) instrument for describing and valuing health status. Participants were asked to indicate which of the following statements best describes their health state:

I have no problems with self-care; I have some problems washing or dressing myself; I am unable to wash or dress myself.

In the table below, each row title lists Baseline health status first followed by End of Treatment health status and reports the number of patients in that category.

Change From Baseline in European Quality of Life-5 Dimensions (EQ-5D) Anxiety/Depression ScoreBaseline and Week 12

The EQ-5D is an international, standardized, nondisease-specific (i.e., generic) instrument for describing and valuing health status. Participants were asked to indicate which of the following statements best describes their health state:

I am not anxious or depressed; I am moderately anxious or depressed; I am extremely anxious or depressed. In the table below, each row title lists Baseline health status first followed by End of Treatment health status and reports the number of patients in that category.

Change From Baseline in European Quality of Life-5 Dimensions (EQ-5D) Usual Activities ScoreBaseline and Week 12

The EQ-5D is a standardized, nondisease-specific instrument for describing health status. Participants were asked which statement best describes their health state with regard to usual activities (work, study or leisure): I have no problems performing my usual activities; I have some problems performing my usual activities; I am unable to perform my usual activities.

In the table below, each row title lists Baseline health status first followed by End of Treatment health status and reports the number of patients in that category.

Change From Baseline in European Quality of Life-5 Dimensions (EQ-5D) Pain/Discomfort ScoreBaseline and Week 12

The EQ-5D is an international, standardized, nondisease-specific (i.e., generic) instrument for describing and valuing health status. Participants were asked to indicate which of the following statements best describes their health state:

I have no pain or discomfort; I have moderate pain or discomfort; I have extreme pain or discomfort. In the table below, each row title lists Baseline health status first followed by End of Treatment health status and reports the number of participants in that category.

Change From Baseline to End of Treatment in Work Productivity and Activity Impairment (WPAI)Baseline and Week 12

This 6-item assessment measures productivity losses during the past 7 days and includes measures on work time missed due to health, impairment while working due to health (the participant's assessment of the degree to which health affected their productivity while working), overall work impairment due to health (takes into account both hours missed due to health and the participant's assessment of the degree to which health affected their productivity while working) and activity impairment due to health (the degree in which health problems affected their ability to do regular daily activities). Scores for each measure are expressed from 0 to 100 with higher numbers indicating greater impairment and less productivity, i.e., worse outcomes. A negative change from baseline indicates improvement.

Trial Locations

Locations (133)

FR33111

🇫🇷

Paris Cedex 13, France

SK42109

🇸🇰

Banska Bystrica, Slovakia

SK42106

🇸🇰

Piestany, Slovakia

SK42102

🇸🇰

Presov, Slovakia

UA38102

🇺🇦

Donetsk, Ukraine

GB44103

🇬🇧

Bristol, United Kingdom

GB44104

🇬🇧

Nantwich, United Kingdom

ES34109

🇪🇸

Madrid, Spain

ES34103

🇪🇸

Madrid, Spain

SK42112

🇸🇰

Bratislava, Slovakia

ES34101

🇪🇸

Madrid, Spain

GB44108

🇬🇧

Cambridge, United Kingdom

GB44106

🇬🇧

Garston, United Kingdom

GB44107

🇬🇧

Plymouth, United Kingdom

UA38104

🇺🇦

Dnepropetrovsk, Ukraine

SE46101

🇸🇪

Gothenburg, Sweden

SE46103

🇸🇪

Karlshamn, Sweden

ES34105

🇪🇸

Pamplona, Spain

SE46104

🇸🇪

Malmo, Sweden

SE46102

🇸🇪

Stockholm, Sweden

GB44101

🇬🇧

Reading, United Kingdom

SK42105

🇸🇰

Pieštany, Slovakia

GB44110

🇬🇧

Northwood, United Kingdom

ES34104

🇪🇸

San Juan de Alicante, Spain

ES34102

🇪🇸

Madrid, Spain

ES34107

🇪🇸

Sevilla, Spain

SE46105

🇸🇪

Tanumshede, Sweden

GB44111

🇬🇧

Glasgow, United Kingdom

GB44105

🇬🇧

Sandbach, United Kingdom

CZ42006

🇨🇿

Plzen, Czechia

DK45104

🇩🇰

Holstebro, Denmark

BY37101

🇧🇾

Minsk, Belarus

BY37102

🇧🇾

Minsk, Belarus

BE32102

🇧🇪

Brussels, Belgium

BE32104

🇧🇪

Edegem, Belgium

CZ42005

🇨🇿

Bohumín, Czechia

CZ42003

🇨🇿

Hradec Kralove, Czechia

CZ42011

🇨🇿

Ostrava, Czechia

CZ42007

🇨🇿

Prague 4, Czechia

CZ42001

🇨🇿

Prague, Czechia

CZ42009

🇨🇿

Prague, Czechia

CZ42010

🇨🇿

Roudnice nad Labem, Czechia

CZ42012

🇨🇿

Sternberk, Czechia

CZ42002

🇨🇿

Uherske Hradiste, Czechia

FI35801

🇫🇮

Oulu, Finland

FR33108

🇫🇷

Dijon, France

FR33103

🇫🇷

Orleans, France

FR33112

🇫🇷

Paris cedex 20, France

FR33106

🇫🇷

Toulouse, France

DE49109

🇩🇪

Bad Ems, Germany

DE49103

🇩🇪

Göttingen, Germany

DE49117

🇩🇪

Hagenow, Germany

DE49105

🇩🇪

Hettstedt, Germany

DE49110

🇩🇪

Neustadt I. Sachsen, Germany

DE49108

🇩🇪

Leipzig, Germany

DE49118

🇩🇪

Reutlingen, Germany

DE49101

🇩🇪

Rostock, Germany

DE49111

🇩🇪

Sangerhausen, Germany

HU36108

🇭🇺

Csongrád, Hungary

HU36106

🇭🇺

Körmend, Hungary

HU36110

🇭🇺

Miskolc, Hungary

HU36103

🇭🇺

Szekszárd, Hungary

HU36107

🇭🇺

Tatabánya, Hungary

NL31102

🇳🇱

Sneek, Netherlands

NL31101

🇳🇱

Winterswijk, Netherlands

NO47104

🇳🇴

Elverum, Norway

PL48108

🇵🇱

Lublin, Poland

PL48106

🇵🇱

Piaseczno, Poland

PL48104

🇵🇱

Pulawy, Poland

PL48105

🇵🇱

Warsaw, Poland

PT35105

🇵🇹

Coimbra, Portugal

PT35104

🇵🇹

Lisbon, Portugal

PT35101

🇵🇹

Porto, Portugal

PT35106

🇵🇹

Tomar, Portugal

RO40102

🇷🇴

Bucharest, Romania

RO40106

🇷🇴

Brasov, Romania

RO40104

🇷🇴

Bucharest, Romania

RO40108

🇷🇴

Bucharest, Romania

RO40105

🇷🇴

Craiova, Romania

RU70108

🇷🇺

Moscow, Russian Federation

RU70110

🇷🇺

Moscow, Russian Federation

RU70101

🇷🇺

Saint Petersburg, Russian Federation

RU70106

🇷🇺

St. Petersburg, Russian Federation

RU70113

🇷🇺

Ufa, Russian Federation

DK45101

🇩🇰

Aarhus N, Denmark

DK45102

🇩🇰

Herlev, Denmark

IT39103

🇮🇹

Avellino, Italy

IT39101

🇮🇹

Catanzaro, Italy

IT39105

🇮🇹

Florence, Italy

IT39102

🇮🇹

Treviglio (BG), Italy

FR33104

🇫🇷

Colmar Cedex, France

FR33110

🇫🇷

Tours, France

BE32103

🇧🇪

Gent, Belgium

FI35803

🇫🇮

Helsinki, Finland

BY37103

🇧🇾

Minsk, Belarus

BY37104

🇧🇾

Vitebsk, Belarus

BE32101

🇧🇪

Leuven, Belgium

FI35804

🇫🇮

Kouvola, Finland

FI35802

🇫🇮

Tampere, Finland

NL31104

🇳🇱

Amsterdam, Netherlands

NL31106

🇳🇱

Maastricht, Netherlands

PL48101

🇵🇱

Warsaw, Poland

HU36101

🇭🇺

Gyor, Hungary

NO47102

🇳🇴

Hamar, Norway

PL48107

🇵🇱

Krakow, Poland

PL48103

🇵🇱

Lodz, Poland

PL48112

🇵🇱

Wiecbork, Poland

HU36104

🇭🇺

Sopron, Hungary

UA38109

🇺🇦

Kiev, Ukraine

PL48111

🇵🇱

Wroclaw, Poland

PT35102

🇵🇹

Coimbra, Portugal

SK42108

🇸🇰

Trencin, Slovakia

SK42103

🇸🇰

Zilina, Slovakia

UA38107

🇺🇦

Lviv, Ukraine

UA38101

🇺🇦

Odessa, Ukraine

PT35107

🇵🇹

Lisbon, Portugal

RO40103

🇷🇴

Bucharest, Romania

RO40101

🇷🇴

Craiova, Romania

RU70112

🇷🇺

Kazan, Russian Federation

RU70102

🇷🇺

Saint Petersburg, Russian Federation

SK42107

🇸🇰

Kosice, Slovakia

PT35110

🇵🇹

Porto, Portugal

RU70109

🇷🇺

St. Petersburg, Russian Federation

SK42104

🇸🇰

Nitra, Slovakia

UA38111

🇺🇦

Donetsk, Ukraine

RO40107

🇷🇴

Sibiu, Romania

RU70103

🇷🇺

Saint Petersburg, Russian Federation

SK42113

🇸🇰

Malacky, Slovakia

RU70107

🇷🇺

Saint Petersburg, Russian Federation

SK42101

🇸🇰

Trenčín, Slovakia

UA38103

🇺🇦

Zaporizhzhya, Ukraine

UA38106

🇺🇦

Kiev, Ukraine

DE49104

🇩🇪

Wismar, Germany

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