Effects of NAC Supplementation on Skeletal Muscle Performance Following Aseptic Injury Induced by Exercise
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 2
- 主要终点
- Change in reduced glutathione in blood
研究概览
简要总结
In this investigation the investigators utilized NAC administration to foster GSH availability during an 8-day period following eccentric exercise-induced muscle damage in order to test our hypotheses: i) antioxidant supplementation does not disturb performance and adaptations induced by exercise-induced muscle injury and ii) redox status perturbations in skeletal muscle are pivotal for the regulation of muscle' inflammatory response and repair.
详细描述
The major thiol-disulfide couple of reduced (GSH) and oxidized glutathione (GSSG) is a key-regulator of major transcriptional pathways regulating aseptic inflammation and recovery of skeletal muscle following aseptic injury. Antioxidant supplementation may hamper exercise-induced cellular adaptations.
Our objective was to examine how thiol-based antioxidant supplementation affects skeletal muscle's performance and redox-sensitive signalling during the inflammatory and repair phases associated with exercise-induced micro-trauma.In a double-blind, counterbalanced design, 12 men received placebo (PLA) or N-acetylcysteine (NAC, 20 mg/kg/day) following muscle-damaging exercise (300 eccentric contractions). In each trial, muscle performance was measured at baseline, post-exercise, 2h post-exercise and daily for 8 consecutive days. Muscle biopsies from vastus lateralis and blood samples were collected pre-exercise and 2h, 2d, and 8d post-exercise.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- Double (Participant, Outcomes Assessor)
入排标准
- 年龄范围
- 18 Years 至 30 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •a) recreationally trained as evidenced by their maximal oxygen consumption levels (VO2max >45 ml/kg/min), b) were engaged in systematic exercise at least three times/week for ≥12 months), c) non-smokers, d) abstained from any vigorous physical activity during the study, e)abstained from consumption of caffeine, alcohol, performance-enhancing or antioxidant supplements, and medications during the study.
排除标准
- •a) a known NAC intolerance or allergy, b) a recent febrile illness, c) history of muscle lesion, d) lower limb trauma
结局指标
主要结局
Change in reduced glutathione in blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Concentration of reduced glutathione in red blood cells
Change in reduced glutathione in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
concentration of reduced glutathione in quadriceps skeletal muscle group
Change in protein carbonyls in red blood cells and serum
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
concentration of protein carbonyls
Change in protein carbonyls in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
protein carbonyl concentration in vastus lateralis skeletal muscle
Change in thiobarbituric acid reactive substances in red blood cells and serum
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
thiobarbituric acid reactive substances concentration in serum and red blood cells
Change in thiobarbituric acid reactive substances in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
thiobarbituric acid reactive substances concentration in vastus lateralis skeletal muscle
Change in oxidized glutathione in red blood cells and blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Concentration of oxidized glutathione in red blood cells and whole blood
Change in total antioxidant capacity in serum
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in oxidized glutathione in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
concentration of oxidized glutathione in vastus lateralis skeletal muscle
Change in catalase activity in red blood cells and serum
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in glutathione peroxidase activity in red blood cells
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in creatine kinase activity in plasma
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in C-reactive protein in plasma
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in macrophage infiltration in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
Change in white blood cell count in blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in neutrophil count in blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in fatty acid binding protein in plasma
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in cortisol concentration in blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in testosterone concentration in plasma
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Change in cytokine concentration in plasma
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Measurement of IL-1β, IL-4, IL-6, TNF-α, IL-8, IL-10, IL-12p70 concentrations in plasma
Change in adhesion molecule concentration in blood
时间窗: one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Measurement of ICAM-1, VCAM-1, sP-selectin, sE-selectin concentrations in plasma
Change in intracellular signalling proteins in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
Measurement of phosphorylation levels of protein kinase B (Akt), mammalian target of rapamycin (mTOR), serine/threonine kinase (p70S6K), ribosomal protein S6 (rpS6), nuclear factor κB (NFκB), serine⁄threonine mitogen activated protein kinase (p38-MAPK) in vastus lateralis muscle.
Change in myogenic determination factor (MyoD) protein levels in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
MyoD expression in vastus lateralis muscle
Change in tumor necrosis factor α in muscle
时间窗: one hour before exercise, 2 hours post-exercise, 2 days post-exercise, 8 days post-exercise
Protein levels of TNF-α in vastus lateralis muscle
次要结局
- Change in muscle function of knee extensor and flexor muscle(one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise)
- Body composition(One day before exercise)
- Maximal aerobic capacity(One day before exercise)
- Change in profile of dietary intake(one hour before exercise, daily for 8 days post-exercise)
- Change in side effect occurence(one hour before exercise, daily for 8 days post-exercise)
研究者
Ioannis G. Fatouros
Assistant Professor
Democritus University of Thrace