Genetic Mutations and Environmental Exposure in Young Patients With Retinoblastoma and in Their Parents and Young Healthy Unrelated Volunteers
- Conditions
- Recurrent RetinoblastomaExtraocular RetinoblastomaIntraocular Retinoblastoma
- Interventions
- Other: Laboratory Biomarker AnalysisOther: Questionnaire Administration
- Registration Number
- NCT00690469
- Lead Sponsor
- Children's Oncology Group
- Brief Summary
This laboratory study is looking at genetic mutations and environmental exposure in young patients with retinoblastoma and in their parents and young healthy unrelated volunteers. Gathering information about gene mutations and environmental exposure may help doctors learn more about the causes of retinoblastoma in young patients.
- Detailed Description
OBJECTIVES:
I. To investigate the role of genotypes for carcinogen metabolizing enzymes (CME) and DNA repair proteins(DRPs) of the father of children diagnosed with retinoblastoma (RB) and his environmental exposures prior to the child?s conception in the etiology of sporadic bilateral retinoblastoma.
II. To test if the prevalence of preconception environmental exposures and polymorphisms with known or predicted functional consequences in genes for CMEs and DRPs is different in fathers of children with sporadic bilateral RB compared with fathers of the control group.
III. To test if the prevalence of the father?s preconception environmental exposures and his polymorphisms in CMEs and DRPs differs between subsets of cases defined by the type of mutation at the RB1 gene locus.
IV. To investigate the role of genotypes for CMEs and DRPs of the mother and child and environmental exposures after the child?s conception in the etiology of sporadic unilateral RB.
V. To test if the prevalence of environmental exposures during the pregnancy and polymorphisms with known or predicted functional consequences in CMEs is different in the mothers of children with sporadic unilateral RB compared with mothers of the control group.
VI. To test if the prevalence of polymorphisms in genes for CMEs and DRPs with known or predicted functional consequences is different in the children with sporadic unilateral RB compared with controls.
VII. To test if the prevalence of gestational exposures and polymorphisms in genes for CMEs of the mother and the polymorphisms in genes for CME and DRPs in the children differs between subsets of cases defined by the type of mutation at the RB1 gene locus.
OUTLINE: This is a multicenter study.
Participants undergo a structured telephone interview questionnaire. The parental questionnaires collect basic demographic data (including age, race, education, and income), occupational history, medical radiation exposure, diet and supplement use (for the year before pregnancy for father, during pregnancy for mother), tobacco use, and alcohol use. The mothers are also asked about residential pesticides and prior assisted reproductive technology.
Controls (parents) provide saliva samples. If a patient is also enrolled on COG-ARET0332, then the patient blood and tumor samples should be submitted. Parents of patients on this protocol should also submit a blood sample. Blood samples from the affected child, and blood and/or sputum samples from the parents may be submitted. Tumor specimens should be submitted if available.
For some patients, a RB1 mutation detection assay on DNA derived from peripheral blood is performed. If the mutation is found, the parents? DNA is also screened. Blood samples undergo DNA-based sequencing analysis, single nucleotide polymorphism genotyping, quantitative Southern blot analysis, isolation of RNA and reverse transcriptase-polymerase chain reaction analysis, and loss of heterozygosity analysis.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 234
-
Cases must meet the following criteria:
-
Diagnosed with sporadic retinoblastoma (RB) on or after 07/01/2006
- No familial retinoblastoma
-
Have permission of physician to contact the parents
-
Diagnosed and/or treated at a Children's Oncology Group (COG) institution or *Wills Eye Hospital
-
-
Controls must meet 1 of the following criteria:
- Mother of a child with unilateral RB
- Father of a child with bilateral RB
- Age-matched non-blood-related child if possible
-
Must reside in the U.S. or Canada
-
Must have telephone in the home
-
Biological parent speaks English or Spanish
-
Concurrent treatment on a therapeutic trial is NOT required
Not provided
Study & Design
- Study Type
- OBSERVATIONAL
- Study Design
- Not specified
- Arm && Interventions
Group Intervention Description Observational (biomarker analysis) Laboratory Biomarker Analysis Participants undergo a structured telephone interview questionnaire. The parental questionnaires collect basic demographic data (including age, race, education, and income), occupational history, medical radiation exposure, diet and supplement use (for the year before pregnancy for father, during pregnancy for mother), tobacco use, and alcohol use. The mothers are also asked about residential pesticides and prior assisted reproductive technology. Controls (parents) provide saliva samples. If a patient is also enrolled on COG-ARET0332, then the patient blood and tumor samples should be submitted. Parents of patients on this protocol should also submit a blood sample. Blood samples from the affected child, and blood and/or sputum samples from the parents may be submitted. Tumor specimens should be submitted if available. For some patients, a RB1 mutation detection assay on DNA derived from peripheral blood is performed. If the mutation is found, the parents? DNA is also screened. Observational (biomarker analysis) Questionnaire Administration Participants undergo a structured telephone interview questionnaire. The parental questionnaires collect basic demographic data (including age, race, education, and income), occupational history, medical radiation exposure, diet and supplement use (for the year before pregnancy for father, during pregnancy for mother), tobacco use, and alcohol use. The mothers are also asked about residential pesticides and prior assisted reproductive technology. Controls (parents) provide saliva samples. If a patient is also enrolled on COG-ARET0332, then the patient blood and tumor samples should be submitted. Parents of patients on this protocol should also submit a blood sample. Blood samples from the affected child, and blood and/or sputum samples from the parents may be submitted. Tumor specimens should be submitted if available. For some patients, a RB1 mutation detection assay on DNA derived from peripheral blood is performed. If the mutation is found, the parents? DNA is also screened.
- Primary Outcome Measures
Name Time Method Probability that mothers of unilateral RB cases are more likely to have specific DNA-repair gene variants than the mothers of the control group Not Provided Significant effect of specific DNA repair and CME genotypes on the risk of unilateral RB Not Provided Association of the probability of having a child with bilateral retinoblastoma (RB) with the paternal genotype for selected DNA repair and carcinogen metabolizing enzymes (CME) genes Not Provided Probability that the bilateral RB1 mutation subtype will vary by DNA repair or CME genotype or preconception exposures of the fathers of bilateral RB cases Not Provided Probability that the unilateral RB1 mutation subtype will vary by DNA repair or CME genotype of the mother, of the affected child, and with gestational exposures Not Provided
- Secondary Outcome Measures
Name Time Method
Trial Locations
- Locations (52)
Children's Healthcare of Atlanta - Egleston
🇺🇸Atlanta, Georgia, United States
Emory University Hospital/Winship Cancer Institute
🇺🇸Atlanta, Georgia, United States
University of Missouri - Ellis Fischel
🇺🇸Columbia, Missouri, United States
Rainbow Babies and Childrens Hospital
🇺🇸Cleveland, Ohio, United States
Children's Hospital of Philadelphia
🇺🇸Philadelphia, Pennsylvania, United States
Driscoll Children's Hospital
🇺🇸Corpus Christi, Texas, United States
Children's Hospital of Pittsburgh of UPMC
🇺🇸Pittsburgh, Pennsylvania, United States
Texas Tech University Health Sciences Center-Amarillo
🇺🇸Amarillo, Texas, United States
Nationwide Children's Hospital
🇺🇸Columbus, Ohio, United States
Nemours Children's Clinic-Jacksonville
🇺🇸Jacksonville, Florida, United States
Nemours Children's Clinic - Pensacola
🇺🇸Pensacola, Florida, United States
Washington University School of Medicine
🇺🇸Saint Louis, Missouri, United States
Connecticut Children's Medical Center
🇺🇸Hartford, Connecticut, United States
Saint Mary's Hospital
🇺🇸West Palm Beach, Florida, United States
Spectrum Health at Butterworth Campus
🇺🇸Grand Rapids, Michigan, United States
San Jorge Children's Hospital
🇵🇷San Juan, Puerto Rico
MedStar Georgetown University Hospital
🇺🇸Washington, District of Columbia, United States
University of Mississippi Medical Center
🇺🇸Jackson, Mississippi, United States
IWK Health Centre
🇨🇦Halifax, Nova Scotia, Canada
British Columbia Children's Hospital
🇨🇦Vancouver, British Columbia, Canada
UCSF Medical Center-Mount Zion
🇺🇸San Francisco, California, United States
University of Miami Miller School of Medicine-Sylvester Cancer Center
🇺🇸Miami, Florida, United States
Indiana University/Melvin and Bren Simon Cancer Center
🇺🇸Indianapolis, Indiana, United States
Wayne State University/Karmanos Cancer Institute
🇺🇸Detroit, Michigan, United States
University of Minnesota/Masonic Cancer Center
🇺🇸Minneapolis, Minnesota, United States
Nevada Cancer Research Foundation CCOP
🇺🇸Las Vegas, Nevada, United States
University of Texas Health Science Center at San Antonio
🇺🇸San Antonio, Texas, United States
Alfred I duPont Hospital for Children
🇺🇸Wilmington, Delaware, United States
Children's Hospital Los Angeles
🇺🇸Los Angeles, California, United States
Nemours Children's Clinic - Orlando
🇺🇸Orlando, Florida, United States
Johns Hopkins All Children's Hospital
🇺🇸Saint Petersburg, Florida, United States
Sacred Heart Hospital
🇺🇸Pensacola, Florida, United States
University of Illinois
🇺🇸Chicago, Illinois, United States
Helen DeVos Children's Hospital at Spectrum Health
🇺🇸Grand Rapids, Michigan, United States
Dayton Children's Hospital
🇺🇸Dayton, Ohio, United States
Children's Hospital Colorado
🇺🇸Aurora, Colorado, United States
Saint Joseph's Hospital/Children's Hospital-Tampa
🇺🇸Tampa, Florida, United States
Mayo Clinic
🇺🇸Rochester, Minnesota, United States
Children's Hospital Central California
🇺🇸Madera, California, United States
UCSF Medical Center-Parnassus
🇺🇸San Francisco, California, United States
Lurie Children's Hospital-Chicago
🇺🇸Chicago, Illinois, United States
University of Iowa/Holden Comprehensive Cancer Center
🇺🇸Iowa City, Iowa, United States
Riley Hospital for Children
🇺🇸Indianapolis, Indiana, United States
University of Kentucky/Markey Cancer Center
🇺🇸Lexington, Kentucky, United States
Columbia Regional
🇺🇸Columbia, Missouri, United States
The Childrens Mercy Hospital
🇺🇸Kansas City, Missouri, United States
University of New Mexico Cancer Center
🇺🇸Albuquerque, New Mexico, United States
Memorial Sloan Kettering Cancer Center
🇺🇸New York, New York, United States
Duke University Medical Center
🇺🇸Durham, North Carolina, United States
Cincinnati Children's Hospital Medical Center
🇺🇸Cincinnati, Ohio, United States
Cleveland Clinic Foundation
🇺🇸Cleveland, Ohio, United States
Cook Children's Medical Center
🇺🇸Fort Worth, Texas, United States