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Real-time Early Detection of Nephrotoxicity by Urinary Biomarker Analysis With SeroFlow Technology

Not Applicable
Recruiting
Conditions
Acute Kidney Injury
Interventions
Diagnostic Test: AKI risk screening using RenaFAST POCT test kits
Registration Number
NCT06124885
Lead Sponsor
National University Hospital, Singapore
Brief Summary

The study aims to perform real-time validation of the RenaFAST kit (a point-of-care test kit that quantifies three urinary proteins) in predicting acute kidney injury(AKI) among patients prescribed drug therapies of nephrotoxic potential. Based on the type and duration of drug therapy, a maximum of 5 time-point urine samples will be collected from consenting patients and a real-time biomarker analysis will be conducted using the RenaFAST kits.

Detailed Description

All eligible patients who fulfill the inclusion and exclusion criteria will be approached for consent. The patients with the highest AKI risk (with all 3 urine biomarkers, Clusterin, monocyte chemoattractant protein-1 (MCP1), and Beta-2 microglobulin (ß2MG), above prediction threshold set by the study) will be identified. The nephrology consultants within the research team will perform a medical chart and physical review(where required) of these patients, noting potential actions to be taken in data collection forms. This will help in evaluating if indeed there are perceived interventions that could potentially be delivered in response to early prediction of AKI.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
150
Inclusion Criteria
  • Patients who receive a projected ≥7 days of therapy of antimicrobials including aminoglycosides (i.e., gentamicin or amikacin), vancomycin, polymyxin, amphotericin and foscarnet.
  • Patients who receive a projected ≥7 days of Calcineurin inhibitors (cyclosporin, tacrolimus)
  • Patients who receive a projected ≥7 days of anti-virals (Cidofovir and Ganciclovir)
  • Patients who receive Anti-cancer drugs (Chemotherapy such as cisplatin, Ifosfamide, Methotrexate, Pemetrexed) or
  • Patients who receive Anti-cancer drugs (Immunotherapy such as immune checkpoint inhibitors as well as types of VGEF inhibitors that are associated with acute kidney injury)
Exclusion Criteria
  • Patients with AKI prior to therapy initiation.
  • Patients with baseline eGFR < 15 mL/min/1.73m2 (stage 5 chronic kidney disease)
  • Patients admitted to intensive care unit at study baseline, as critical illness is a natural confounder to AKI
  • Females who are pregnant
  • Immediate post-kidney transplant recipients (initial 3 months following transplant).

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
AKI risk screening using RenaFAST POCT test kitsAKI risk screening using RenaFAST POCT test kitsAll consenting patients will have a real-time biomarker analysis done at 5 time-points during their course of drug therapy using the renaFAST kits.
Primary Outcome Measures
NameTimeMethod
Presence of an Acute kidney injury (AKI) eventStart date of drug therapy till one week post end date of drug therapy

AKI will be defined by the minimum stage 1 criterion in accordance to KDIGO AKI criteria:

1. Relative increase in serum creatinine of 1.5 times or higher, versus the baseline.

2. Absolute increase in serum creatinine of \> 26.5 μmol/L within 48 hours.

Additionally, for those administered cisplatin, cases of severe hyponatremia needing hospitalization for severe dehydration and intravenous fluid-rescue will also be taken as an outcome measure of clinically-evident kidney injury and renal salt wasting.

If the subject meets any one of the above 3 criteria, the patient will be recorded as having suffered an AKI event.

Secondary Outcome Measures
NameTimeMethod
Number of patients requiring dialysis treatment for the AKI eventFrom the date of AKI onset to date of resolution of AKI

Whether patient required dialysis/CRRT for treatment of AKI event

Severity of AKI eventFrom the date of AKI onset to date of peak AKI

Peak AKI severity will be determined by highest recorded serum creatinine levels of patient.

Number of AKI days till recoveryFrom the date of AKI onset to date of resolution of AKI

Number of days from the onset of AKI (as per aforementioned AKI criteria) to resolution of AKI (Defined as when serum creatinine levels reach baseline levels or no longer meet the AKI criterion, whichever is earlier)

Length of stay in hospitalFrom the date of admission to the date of discharge of patient from hospital, assessed up to 12months from date of consent

Duration of hospital stay will be determined based on admission and discharge dates of the patient, for the period relevant to study participation.

Trial Locations

Locations (1)

National University Hospital

🇸🇬

Singapore, Singapore

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