Determination of dihydropyrimidine dehydrogenase and thymidylate synthase activity in human peripheral blood mononuclear cells in healthy volunteers.
Completed
- Conditions
- DPD and TS enzyme activity1003754610027655
- Registration Number
- NL-OMON36760
- Lead Sponsor
- Antoni van Leeuwenhoek Ziekenhuis
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 40
Inclusion Criteria
1. Healthy volunteer; not known with cancer or current treatment for cancer, and without any surgical operation in the past 6 months
2. Age > 18 years
3. Able and willing to give written informed consent
4. Able and willing to undergo blood sampling for DPD enzyme activity analysis
5. Able and willing to undergo blood sampling for pharmacogenetic analysis
Exclusion Criteria
1. Any treatment with investigational drugs within 30 days before the start of the study
2. History of cancer
3. Any treatment with anti-cancer drugs
4. Legal incapacity
5. Any condition that may interfere with the study protocol
Study & Design
- Study Type
- Observational invasive
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>ranges of and mean DPD resp. TS enzyme activity in healthy volunteers</p><br>
- Secondary Outcome Measures
Name Time Method <p>Correlation of DPD and TS enzyme activity with DPD and TS gene expression and<br /><br>observed polymorphisms in DPYD resp. TYMS.</p><br>