Combustion Derived Air Pollution and Vascular Function
- Conditions
- Endothelial Dysfunction
- Interventions
- Procedure: Forearm Vascular StudyProcedure: Badimon Chamber
- Registration Number
- NCT00775099
- Lead Sponsor
- University of Edinburgh
- Brief Summary
Air pollution is a major cause of cardiovascular morbidity and mortality. The components of air pollution responsible and the mechanisms through which they might mediate these harmful effects remain only partially understood. The link between cardiovascular disease and air pollution is strongest for fine particulate matter. Fine particulate matter (PM) is produced from the combustion of fossil fuels with the most significant threat thought to be posed by small particles less than 10µm (PM 10) which can be inhaled into the lungs. We propose to identify the precise component of diesel exhaust that mediates the adverse cardiovascular effects using a carbon particle generator, and a particle concentrator. The aim of this study proposal is to assess the vascular effects of different types and components of air pollution in healthy subjects. We intend to test the hypotheses that:
1. Combustion derived nanoparticulate causes an acute impairment of endothelial vasomotor and fibrinolytic function in healthy volunteers.
2. Exposure to combustion derived air pollution is associated with increased thrombus formation.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 16
- Healthy volunteers
- Current smokers
- Significant occupational exposure to air pollution
- History of lung disease
- Women of child-bearing potential
- Malignant arrhythmias
- Renal or hepatic failure
- Significant co-morbidity
- Systolic blood pressure >190 or <100 mmHg
- Previous history of blood dyscrasia
- Unable to tolerate the supine position
- Lack of informed consent
- Blood donation within last 3 months
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Filtered Air Exposure Forearm Vascular Study 1 hour exposure to filtered air during intermittent exercise Filtered Air Exposure Badimon Chamber 1 hour exposure to filtered air during intermittent exercise Diesel Exhaust Exposure Forearm Vascular Study 1 hour exposure to dilute diesel exhaust at a concentration of 300 µg/m3 during intermittent exercise Diesel Exhaust Exposure Badimon Chamber 1 hour exposure to dilute diesel exhaust at a concentration of 300 µg/m3 during intermittent exercise Filtered Diesel Exposure Forearm Vascular Study 1 hour exposure to diesel exhaust with all particulates filtered out using teflon filter with intermittent exercise Filtered Diesel Exposure Badimon Chamber 1 hour exposure to diesel exhaust with all particulates filtered out using teflon filter with intermittent exercise PALAS Exposure Forearm Vascular Study 1 hour exposure to pure carbon particles produced by PALAS generator during intermittent exercise PALAS Exposure Badimon Chamber 1 hour exposure to pure carbon particles produced by PALAS generator during intermittent exercise
- Primary Outcome Measures
Name Time Method Forearm blood flow measured by forearm venous occlusion plethysmography in response to infused vasodilators 6-8 hours after exposure
- Secondary Outcome Measures
Name Time Method Heart rate and heart rate variability measured with 3 lead Holter electrographic monitors During and for 24 hours after exposure Ex-vivo thrombus formation assessed using the Badimon chamber 6 hours after exposure Blood pressure During and after exposure and during forearm study Plasma t-PA and PAI concentrations following infusion of bradykinin During forearm study Arterial stiffness measured by radial artery tonometry Before and after exposure Plasma inflammatory markers IL-6, TNF-alpha, IL-1 and hsCRP Before and after exposure Platelet monocyte binding as measured by flow cytometry After the exposure
Trial Locations
- Locations (1)
University of Edinburgh
🇬🇧Edinburgh, United Kingdom