Microarray Analysis of Sinus Samples From Patients With and Without Chronic Rhinosinusitis
- Conditions
- Maxillary Sinusitis
- Registration Number
- NCT00683371
- Lead Sponsor
- University of California, San Francisco
- Brief Summary
Mucosal biopsies, endoscopically-guided brush samples of mucus, and a saline lavage taken from the maxillary sinuses of ten CRS patients undergoing sinus surgery are analyzed using three microarrays in order to detect bacteria, fungi and viruses. Ten control patients with normal sinuses will have the same samples taken. The hypothesis is that bacterial, fungal, and viral communities present in the maxillary sinus of patients with CRS are significantly different from those patients with healthy sinuses, and that microorganisms identified in patients with or without CRS will differ from previously published data obtained using other techniques.
- Detailed Description
Mucosal biopsies, endoscopically-guided brush samples of mucus, and a saline lavage taken from the maxillary sinuses of ten CRS patients undergoing sinus surgery are analyzed using three microarrays: 16S rRNA PhyloChip (to detect bacteria), MycoChip (to detect fungi) and ViroChip (to detect viruses). Ten control patients with normal sinuses, as assessed by CT scan and a sinusitis-specific survey, will have the same samples taken. The hypothesis is that bacterial, fungal, and viral communities present in the maxillary sinus of patients with CRS are significantly different from those patients with healthy sinuses, and that microorganisms identified in patients with or without CRS will differ from previously published data obtained using other techniques. Comparing diseased and control flora will provide insight into the relative contribution of each pathogen to CRS and may guide the development of future therapies.
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 20
Not provided
- Control patients with any evidence of CRS, by history, survey, or imaging criteria would be excluded.
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- PARALLEL
- Primary Outcome Measures
Name Time Method Microbial community profiling using the PhyloChip, MycoChip, and ViroChip will yield vast quantities of data to be reduced in dimensions for interpretation. Pathogens detected in the nasal mucus of patients with CRS will be compared to healthy controls. Immediate Preoperative period.
- Secondary Outcome Measures
Name Time Method The presence or absence of a given microorganism will be compared to previously published data obtained using either traditional culture methods or other genomic methods. Immediate preoperative period
Related Research Topics
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Trial Locations
- Locations (1)
University of California, San Francisco, Dept of Otolaryngology-HNS
🇺🇸San Francisco, California, United States
University of California, San Francisco, Dept of Otolaryngology-HNS🇺🇸San Francisco, California, United States