Proteomic and Metabolomic Lacrimal Fingerprint in Diverse Pathologies of the Ocular Surface
Overview
- Phase
- Not Applicable
- Intervention
- Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
- Conditions
- Dry Eye Syndrome
- Sponsor
- Centre hospitalier de l'Université de Montréal (CHUM)
- Enrollment
- 300
- Locations
- 4
- Primary Endpoint
- To obtain the metabolomic and proteomic fingerprint of the studied ocular surface pathologies
- Status
- Recruiting
- Last Updated
- 5 days ago
Overview
Brief Summary
This study aims to obtain the lacrimal fingerprint for frequent pathologies of the ocular surface and establish a normative base for each of them.
Detailed Description
This is an exploratory study. The investigators aim to: * Test and perfect a tear sample analysis technique by mass spectrometry with samples collected by Schirmer strips; * Obtain the lacrimal fingerprint for various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and establish a normative base for each of them. During regular clinics at the cornea service of the ophthalmology department of the CHUM (Centre Hospitalier de l'Université de Montréal), the investigators aim to recruit a few hundred patients with various pathologies of the ocular surface, notably different forms of dry eye syndrome, infectious keratitis/conjunctivitis, mucous membrane pemphigoid and allergic conjunctivitis, and collect a sample of their tears via Schirmer strip. A case report form will be completed for each patient, noting known ocular diagnoses and active topical ophthalmic medication. Schirmer strips will be sent in sterile tubes to UQAM (Université du Québec à Montréal)'s department of chemistry for analysis by mass spectrometry.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Patients with healthy corneas or suffering from one of these pathologies:
- •Dry eye syndrome; Infectious keratitis and/or conjunctivitis; Mucous membrane pemphigoid; Allergic conjunctivitis.
Exclusion Criteria
- •Patients younger than 18 years old;
- •Patients incapable of giving informed consent.
Arms & Interventions
Control group
Healthy, normal ocular surface
Intervention: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
Dry eye syndrome
Patients suffering from either: * Lacrimal insufficiency * Anterior blepharitis * Posterior blepharitis * Sjögren syndrome
Intervention: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
Allergic conjunctivitis
Patients suffering from allergic conjunctivitis
Intervention: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
Mucous membrane pemphigoid
Patients suffering from mucous membrane pemphigoid
Intervention: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
Infectious keratoconjunctivitis
Patients suffering from keratitis and/or conjunctivitis of various etiology: * Viral * Bacterial * Fungal * Acanthamoeba
Intervention: Tear sample collection via Schirmer strip and subsequent analysis by mass spectrometry
Outcomes
Primary Outcomes
To obtain the metabolomic and proteomic fingerprint of the studied ocular surface pathologies
Time Frame: 5 years
Raw data will be analysed with MarkerView software by Sciex to identify specific over- or under-expressed markers in these ocular pathologies.
Secondary Outcomes
- To objectify changes in metabolomic and proteomic profile associated with topical treatments taken by participants in this study(5 years)