Allogeneic Immunotherapy of Hematological Malignancies Using Regulatory T-cell Selective Depletion

Phase 1

Not yet recruiting

• Conditions: Regulatory T Cell Depletion; Hematological Malignancies; Relapse
• Interventions: Drug: T-reg depleted DLI

• Registration Number: NCT06180499
• Lead Sponsor: Assistance Publique - Hôpitaux de Paris

Brief Summary

Since the discovery that Treg suppress anti-tumor immune responses, inhibiting their function has become a major challenge for the development of efficient immunotherapy for cancer. In humans, we previously reported the positive results of a first clinical trial using Treg depletion for anti-tumor response amplification in the field of allogeneic hematopoietic stem cell transplantation (HSCT). The present project aims at developing this anti-tumor immunotherapeutic strategy in the same setting, i.e. donor lymphocyte infusion (DLI) for relapsing hematological malignancies after HSCT, using a new selection marker: CD127. The choice of this new strategy is supported by our results of a retrospective clinical study and pre-clinical data. Using human cells, this studies demonstrated, in vitro and in vivo in animal murine models, that Treg depletion through CD127 positive selection is much more efficient to improve allogeneic immune responses of donor T-cells as compared to the previous strategy using the CD25 marker.

Detailed Description

Not available

Study Timeline

• Study First Submitted: 2023-12-12
• Study First Submitted QC: 2023-12-12
• Study First Posted: 2023-12-22
• Status Verified: 2024-02
• Last Update Submitted: 2024-02-12
• Last Update Posted: 2024-02-14
• Study Start: 2024-03
• Primary Completion: 2028-11
• Study Completion: 2029-09

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: All
• Target Recruitment: 27

Inclusion Criteria

• Adult patient (older than 18 years old without upper limit of age) diagnosed with leukemia, myelodysplasia, myeloproliferative disorder or lymphoproliferative disorder (CLL, myeloma, lymphoma)
• Previous allogeneic HSCT from a matched sibling, haplo-identical or unrelated donor (any type of conditioning regimen)
• Haematological relapse (molecular, cytogenetic or cytological) after HSCT
• Patient refractory (no or partial response) to one or several previous standard unmanipulated DLI
• Availability of cryopreserved lymphapheresis
• No loss of chance by using of DLI rather than more incisive anti-tumor agents according to investigator appreciation
• Written informed consent before any intervention necessary for the trial
• Affiliation to a social security regime
• Negative pregnancy test for women of childbearing age participating in the study
• Effective contraceptive methods for men / women in line with the current CTFG recommendations version 1.1

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Exclusion Criteria

• Acute grade ≥ II or moderate/severe chronic GVHD at the time of inclusion
• Patient receiving immunosuppressive treatment for GVHD or any other reason
• Creatinine clearance< 50 ml/min
• Serum aspartate aminotransferase (AST)/serum glutamic-oxaloacetic transaminase (SGOT) or alanine transaminase (ALT)/serum glutamate pyruvate transaminase (SGPT) > 5.0 x upper limit of normal (ULN)
• Serum total bilirubin > 50µM (expect for unconjugated hyperbilirubinemia due to Gilbert's disease)
• Performance status ECOG>1
• Severe infection according to CTCAE grading (grade>2)
• Pregnant or lactating women
• Patient under tutorship, curatorship or legal protection
• Ongoing participation in another interventional research protocol within the same field of immune modulation (through cell therapy or not)
• State medical aid

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
T-reg depleted DLI	T-reg depleted DLI	-

Primary Outcome Measures

Name	Time	Method
cumulative incidence of GVHD in its acute grade ≥ II and/or severe chronic form (according Glucksberg-Thomas and NIH scales, respectively), and uncontrolled after a 14-day immunosuppressive course including steroids.	occurring within the 2 months following d-DLI infusion	Composite criteria. In this evaluation, death from non-GVHD cause will be taken as a competitive event

Secondary Outcome Measures

Name	Time	Method
Date of putative relapse/progression for estimation disease-free survival	at 2 and 12 month
Number, causes and date of deaths	at 2 and 12 month
Incidence of acute and/or chronic GVHD, with corresponding grades according to NIH and Glucksberg-Thomas scales	at 2 and 12 month
Date of putative relapse/progression for estimation of cumulative incidence (taking into account the competitive risk of death not related to relapse)	at 2 and 12 month