Thrombogenicity of Lipoprotein A: a Prospective, Non - Randomised, Open Label, Proof of Concept, Laboratory Study Defining the Prothrombotic Effects of Lipoprotein A
试验速览
- 阶段
- 不适用
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- LP(a) thrombogenicity
研究概览
简要总结
Brief summary: Lipoprotein a (Lp(a)) is an independent risk factor for cardiovascular and cerebrovascular disease. Traditionally, the pathogenic role of Lp(a) has been linked to the atherogenic process given its similarity to low density lipoprotein (LDL), however there is a potential for prothrombotic tendencies given its resemblance to plasminogen. The emerging evidence suggests that the prothrombotic properties of Lp(a) contribute not only to arterial but also to venous thrombosis. Lp(a) has the potential to participate in thrombogenesis via several mechanisms: probable platelet aggregation and activation, increased expression of plasminogen activator inhibitor - 1, and reduced production of plasmin. Prior data suggests that Lp(a), can also modify fibrin clot permeability and its susceptibility to lysis. These observations have potentially important implications in patients with a history of myocardial infarction, stroke and venous thromboembolic disease.
The investigators propose to conduct a proof-of-concept study to assess the prothrombotic effects of Lp(a), using both quantitative and qualitative assessment of thrombosis, in particular analysing clot structure and dynamics.
详细描述
Thrombogenicity of Lp(a) study is a two-centre, open-label, non-randomised, observational laboratory study defining the prothrombotic effects of Lp(a). The study is aimed to answer the question of effect of LP(a) on thrombus formation in patients with previous history of myocardial infarction. Blood thrombogenicity in patients with previous history of myocardial infarction will be studied using the following techniques:
i) Badimon chamber: this will assess thrombogenicity of flowing blood. Approximately 50 mls of blood drawn from the antecubital vein, will be passed through the chamber at 37 degrees C. The chamber will contain strips of porcine aorta denuded of its intima layer to promote thrombosis. The resulting thrombus will be fixed in formalin, stained with haematoxylin/eosin and the amount of thrombus quantified using the Image ProPlus software.
ii) Scanning electron microscope (SEM): A sample of the resulting thrombus from the above experiment will be gold stained and the thrombus ultrastructure analysed under SEM. This will allow for examination and analysis of the microstructure morphology and characterization of the thrombus.
iii) Thromboelastography (TEG) is a comprehensive assay of the overall clotting process, a method used to provide a global assessment of whole blood, coagulation and clot lysis.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Only
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Adult patients with stable coronary artery disease and previous history of myocardial infarction currently receiving aspirin monotherapy;
- •Age ≥ 18;
- •Patients who can provide written informed consent for participation in the trial;
排除标准
- •Haematological disorders (anaemia, malignancy, bleeding disorder)
- •Malignancy (currently diagnosed or under investigation)
- •Current smokers
- •Chronic liver disease
- •End stage renal disease (eGFR<30ml/min)
- •Use of corticosteroids or non-steroidal anti-inflammatory agents
- •Patients taking aspirin at dose of above 75mg daily
- •Patients receiving anticoagulant treatment or antiplatelet treatment other than aspirin
- •Unable to consent
结局指标
主要结局
LP(a) thrombogenicity
时间窗: Through study completion, an average of 1 year
Quantity of thrombus measured following Badimon Perfusion Chamber Test
次要结局
未报告次要终点