Impact of Neutrophil Extracellular Traps on Tissue Plasminogen Activator Induced Thrombolysis in Acute Ischemic Stroke Patients

Withdrawn

• Conditions: Acute Stroke

• Registration Number: NCT02907736
• Lead Sponsor: Fondation Ophtalmologique Adolphe de Rothschild

Brief Summary

Acute Ischemic stroke (AIS) remains a leading cause of adult disability, cognitive impairment and mortality worldwide despite the development of revascularization therapies (intravenous Tissue Plasminogen Activator (t-PA) and endovascular therapy). Thrombosis resistance after IV t-PA therapy is frequent especially in case of AIS with proximal occlusion. In recent years, neutrophil extracellular traps (NETs) have been identified as major triggers and structural factors of various forms of thrombosis. NETs are extracellular webs primarily composed of DNA from neutrophils. A recent study shows that the NETs burden in coronary thrombi is positively correlated with the infarct size and negatively correlated with electrocardiogram (ST-segment) resolution. This later study revealed that in vitro addition of DNase I accelerated the t-PA-induced thrombolysis of coronary thrombi. NETs could, in consequence, be promising targets for improved thrombolysis in AIS.

The aim of this study is to assess the impact of NETs composition of thrombi retrieved during endovascular therapy in AIS patients on IV t-PA induced thrombolysis, clinical outcome and AIS etiologies.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-11-12
• Study First Submitted: 2016-09-06
• Study First Submitted QC: 2016-09-15
• Study First Posted: 2016-09-20
• Primary Completion: 2017-07-01
• Study Completion: 2017-07-01
• Status Verified: 2018-03
• Last Update Submitted: 2018-03-16
• Last Update Posted: 2018-03-19

Recruitment & Eligibility

• Status: WITHDRAWN
• Sex: All
• Target Recruitment: Not specified

Inclusion Criteria

• age > 18 years old
• AIS secondary to a large vessel occlusion
• admitted for endovascular therapy.

Exclusion Criteria

• Pregnant or breast feeding patient patient under legal protection
• Patient opposition to participate in this study

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
thrombus origin	baseline	according to the etiology of the stroke (TOAST classification)
red cells counts	baseline	quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining :
white cells counts	baseline	quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining
platlet counts	baseline	quantitative assessment of the relative fractions of the main constituents after Hematoxylin and Eosin staining

Trial Locations

Locations (1)

Fondation Ophtalmologique Adolphe de Rothschild; 🇫🇷 Paris, France