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Impact of Allo- and Autoantibodies on Chronic Cardiac Allograft Function

Completed
Conditions
Pediatric Heart Transplantation
Pediatric Heart Transplant Recipients
Registration Number
NCT02752789
Lead Sponsor
National Institute of Allergy and Infectious Diseases (NIAID)
Brief Summary

This is a multi-center, prospective, single cohort, observational study of pediatric heart transplant recipients designed to determine the impact of preformed versus de novo human leukocyte antigen (HLA) donor-specific antibodies (DSA), and antibodies to the self-antigens cardiac myosin and vimentin, on chronic allograft function. In addition, the investigators will explore mechanisms of action and predictors of DSA, rejection and altered pathophysiology.

Detailed Description

Participants that were enrolled in the CTOTC-04 study (ClinicalTrials.gov Identifier NCT01005316) are invited to enroll in this CTOTC-09 study. Conversion from the CTOTC-04 to CTOTC-09 study will occur in such a manner as to avoid/minimize discontinuity of follow-up between the planned CTOTC-04 and CTOTC-09 study visits. In addition, subjects added to the United Network for Organ Sharing (UNOS) system-or Canadian equivalent agency-at a participating study site, who are less than 21 years of age and fulfill all study eligibility criteria, will be invited to enroll in CTOTC-09.

This study focuses on the importance of antibodies against the newly transplanted heart in pediatric heart transplant recipients. The investigators aim to determine if certain antibodies lead to problems with the heart transplant. Antibodies are small proteins in the blood that the body makes to fight off infections, for example with bacteria or viruses. Since a new heart is "foreign" to the recipient's body, their immune system might try to attack it with antibodies, as if it were an infection. For many years it was thought that only white blood cells attacked the new heart, causing rejection.

Now there is new information showing that antibodies may also cause rejection or long-term damage to the heart. At this time, very little is known about how antibodies might cause problems after heart transplantation in transplant recipients younger than 21 years at the time of transplant.

This study will collect a medical history and blood samples at specified times for research. The blood samples will be used to measure antibodies in the blood, and to perform special tests to see how these antibodies might damage the heart.

Participant follow-up is from the day of the heart transplant to year 5 post-transplant.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
407
Inclusion Criteria
  • Subject and/or parent guardian able to understand and provide informed consent and where applicable assent
  • Planned long-term follow-up at one of the study sites

AND either:

-Enrolled in the CTOTC-04 study and actively followed at one of the study sites

OR

-Listed at participating study sites, less than 21 years of age and not yet transplanted.

The inclusion criteria for enrollment of new study patients in the CTOTC-09 Protocol will be the same as the CTOTC-04 study (refer to ClinicalTrials.gov ID NCT01005316).

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Exclusion Criteria
  • Parental withdrawal of consent from the CTOTC-04 study
  • Past or current medical problems or findings from physical examination or laboratory testing that, in the opinion of the investigator, may pose additional risks from participation in the study, may interfere with the participant's ability to comply with study requirements or may impact the quality or interpretation of the data obtained from the study
  • Listed for simultaneous multiple organ transplant.
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Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Pulmonary capillary wedge pressure at heart catheterization3 years post-transplantation
Secondary Outcome Measures
NameTimeMethod
Time to first episode of late acute rejectionFrom >1 year to 5 years post-transplantation

Late acute rejection is defined as occurring \>1 year post-transplantation

Systolic and diastolic graft function3 and 5 years

Graft function as assessed by echocardiography

Other invasive cardiac hemodynamic findings at cardiac catheterization3 and 5 years post-transplantation

Cardiac hemodynamic findings: right and left ventricular end diastolic pressures, right atrial pressure, pulmonary artery pressure and cardiac index

Frequency of development of post-transplant de novo DSA and autoantibodies to cardiac myosin and vimentin3 years post-transplantation
Frequency to recurrent (two or more) late acute rejectionsUp to 5 years post-transplantation
Frequency to first episode of late acute rejection with hemodynamic compromiseUp to 5 years post-transplantation
N-terminal pro-brain Natriuretic Peptide (NT-proBNP)/Brain Natriuretic Peptide (BNP)3 and 5 years post-transplantation
Time course of development of post-transplant de novo DSA and autoantibodies to cardiac myosin and vimentin.3 years post-transplantation
Time to recurrent late acute rejectionsUp to 5 years post-transplantation

Recurrent defined as two or more late acute rejection episodes

Time to first episode of late acute rejection with hemodynamic compromiseUp to 5 years post-transplantation
Time to graft loss (death or retransplantation) after first late rejectionUp to 5 years post-transplantation
Variability of maintenance tacrolimus levelsUp to 5 years post-transplantation
Frequency of first episode of late acute rejectionFrom >1 year to 5 years post-transplantation
Time to graft loss (death or retransplantation) conditional to surviving one year post-transplantationOne year and up to 5 years post-transplantation
Proportion of participants with angiographic evidence of coronary artery disease3 and 5 years post-transplantation
Medication Adherence Measure (MAM) after hospital dischargeUp to 5 years post-transplantation

Trial Locations

Locations (9)

Emory University School of Medicine

🇺🇸

Atlanta, Georgia, United States

Columbia University Medical Center

🇺🇸

New York, New York, United States

Children's Hospital Boston

🇺🇸

Boston, Massachusetts, United States

St. Louis Children's Hospital

🇺🇸

Saint Louis, Missouri, United States

Monroe Carell Jr. Children's Hospital

🇺🇸

Nashville, Tennessee, United States

Children's Hospital at Montefiore

🇺🇸

New York, New York, United States

Hospital for Sick Children

🇨🇦

Toronto, Canada

Children's Hospital of Pittsburgh

🇺🇸

Pittsburgh, Pennsylvania, United States

Children's Hospital of Philadelphia

🇺🇸

Philadelphia, Pennsylvania, United States

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