Evaluation of the Effect of Non-surgical Periodontal Treatment on Salivary and Gingival Crevicular Fluid Levels of IL-17 and IL-35 in Periodontitis Patients as Smoker and Non-smoker
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 55
- 试验地点
- 4
- 主要终点
- IL-17 levels in gingival crevicular fluid
研究概览
简要总结
Periodontal diseases are among the major causes of tooth loss. Smoking may play a role as a contributing factor in the development of periodontitis by reducing the immune response. The role of cytokines in the pathogenesis of periodontal disease is clearly indicated in the literature; it has been shown that microorganisms that cause periodontal disease cause cytokine increase in saliva, gingival tissue and gingival crevicular fluid. Among these cytokines, interleukin (IL) -17 is proinflammatory and IL-35 is antiinflammatory and has been associated with periodontal disease.
详细描述
Periodontal diseases are among the complex inflammatory diseases that are considered among the leading causes of tooth loss, mostly observed with multiple etiological factors. The complexity of periodontal disease is due to the presence of the biofilm and the accompanying host response together.
Smoking, which is considered as one of the risk factors in terms of periodontitis, is one of the most preventable risk factors that affect the periodonium with many different mechanisms. Smoking plays a role in the etiopathogenesis of periodontitis, with its physiological changes in gingival tissue and its effects on host response and is considered to be an established risk factor. Although the effects of smoking on host response and its role in increasing the risk of periodontal disease are still being investigated, its effects on natural and acquired immune response cells have been studied separately. Consequently, components of cigarette suppress the immune system's defense responses; however, it has been reported to exacerbate pathological reactions.
The process of progression from periodontal health to periodontitis is formed by biofilm pathogens and host immune responses activated by these microorganisms. Intercellular communication is crucial during the progression of chronic inflammatory diseases, such as periodontitis. Cytokines, one of these ways of communication, are soluble proteins produced by various types of cells, such as structural and inflammatory cells, responsible for maintaining a complex network of communication between homotypic and heterotypic cell groups.
Interleukin-17 (IL-17) is a pro-inflammatory cytokine mostly stimulated from Th17 cells, stimulating inflammatory processes with many different mechanisms.
IL-35 inhibits the differentiation of Th17 cells and suppresses IL-17 production and therefore plays a protective role in Th17-related diseases.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Treatment
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •To be volunteer to participate in the study
- •To be over 18
- •Being systemically healthy
- •Having greater than or equal to 15 teeth out of 3rd molar teeth
- •To have localized or generalized periodontitis for the experimental group (clinical attachment loss in at least 6 surrounding areas and presence of greater than or equal to 5 mm periodontal pocket)
- •For Smoking group; more than 10 cigarettes a day for more than 5 years
- •For non-smokers; have not smoked for at least 3 years.
排除标准
- •Have any systemic disease affecting periodontal condition
- •To receive periodontal treatment in the last 6 months
- •Use any medication that may affect the inflammatory process in the last 3 months
- •Use local or systemic antibiotics in the last 3 months
- •Pregnancy or lactation for female patients
- •Regular use of mouthwash
结局指标
主要结局
IL-17 levels in gingival crevicular fluid
时间窗: Change from baseline to 1st month after non-surgical periodontal treatment
by ELISA method
IL-17 levels in saliva
时间窗: Change from baseline to 1st month after non-surgical periodontal treatment
by ELISA method
IL-35 levels in gingival crevicular fluid
时间窗: Change from baseline to 1st month after non-surgical periodontal treatment
by ELISA method
IL-35 levels in saliva
时间窗: Change from baseline to 1st month after non-surgical periodontal treatment
by ELISA method
次要结局
- Plaque Index (PI)(Change from baseline to 1st month after non-surgical periodontal treatment)
- Pocket Depth (PD)(Change from baseline to 1st month after non-surgical periodontal treatment)
- Bleeding in Probing Index (BOP)(Change from baseline to 1st month after non-surgical periodontal treatment)
- Gingival Index (GI)(Change from baseline to 1st month after non-surgical periodontal treatment)
- Clinical Attachment Loss (CAL)(Change from baseline to 1st month after non-surgical periodontal treatment)
研究者
Ezgi Sıla Taşkaldıran
Investigator
Gazi University