Wound Vitality Markers in Forensic Pathology

• Conditions: Wound
• Interventions: Other: Abdominoplasty with exeresis of cutaneous tissue; Other: Collection of cutaneous tissue

• Registration Number: NCT02892526
• Lead Sponsor: Central Hospital, Nancy, France

Brief Summary

The purpose is to determine intrinsic properties of various immunohistochemical markers (FVIIIra, CD15, CD30, tryptase, TNFα, IL-1β, TGFα et TGFβ1) for diagnosis of vital wound, alone and in association (evaluation of sensibility with surgery wounds and evaluation of specificity with post-mortem wounds).

Secondary purposes are to measure the minimum time to obtain a positive labeling in vital wounds, and to evaluate inter-observer reproducibility of vitality diagnosis with different markers. Expression of microRNA miR 9, miR 21 et miR 198 will be also studied.

Detailed Description

Not available

Study Timeline

• Study Start: 2014-12
• Status Verified: 2016-09
• Study First Submitted: 2016-09-02
• Study First Submitted QC: 2016-09-02
• Last Update Submitted: 2016-09-02
• Study First Posted: 2016-09-08
• Last Update Posted: 2016-09-08
• Primary Completion: 2016-12
• Study Completion: 2016-12

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 57

Inclusion Criteria

Alive persons:

• Abdominoplasty

Deceased persons:

• Medico-scientific autopsy for diagnosis

Exclusion Criteria

Alive persons:

• Fragmented sample with non-visible banks
• Cutaneous pathology

Deceased persons:

• Persons under protection
• Medico-legal obstacle
• Cutaneous pathology

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Vital wounds	Abdominoplasty with exeresis of cutaneous tissue	from abdominoplasty of alive persons
Post-mortem wounds	Collection of cutaneous tissue	from autopsy of deceased persons

Primary Outcome Measures

Name	Time	Method
Sensibility of markers for vitality diagnosis	baseline
Specificity of markers for vitality diagnosis	baseline

Secondary Outcome Measures

Name	Time	Method
Expression level of miR 21 by qRT-PCR	baseline
Expression level of miR 9 by qRT-PCR	baseline
Expression level of miR 198 by qRT-PCR	baseline
Minimum time to obtain a labeling (between incision and devascularization)	baseline
Coefficient of inter-observer correlation of vitality diagnosis (for reproducibility analysis)	baseline

Trial Locations

Locations (1)

CHRU Nancy; 🇫🇷 Nancy, France