Effects of BCG vaccination on immunological characteristics of hematopoetic stem cells: an explorative study
Completed
- Conditions
- tuberculosis vaccineBCG vaccine1000225210027665
- Registration Number
- NL-OMON43468
- Lead Sponsor
- Radboud Universitair Medisch Centrum
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Completed
- Sex
- Not specified
- Target Recruitment
- 21
Inclusion Criteria
Age>18
Healthy
Exclusion Criteria
Use of systemic medication other than oral anti-contraceptive drugs
Vaccination within 3 months prior to study period
History of haematological disease
History of malignancy
Medical history of disease associated with immune deficiency
Previous BCG vaccination
Acute illness within 2 months prior to start of study
Pregnancy
Anaemia or other deviations in a complete blood count
NSAID within the last 2 weeks
History of claustrophobia
Fastened glucose >8mmol/l
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method <p>Cellular composition of the bone marrow<br /><br>Ex vivo differentiation of hematopoetic stem and progenitor cells<br /><br>Ex vivo cytokine responses of hematopoetic stem and progenitor cells</p><br>
- Secondary Outcome Measures
Name Time Method <p>Ex vivo cytokine production of PBMCs and monocytes<br /><br>Gene expression profiles of hematopoetic stem cells and peripheral monocytes<br /><br>Epigenetic profiles relating to immune function of hematopoetic stem cells and<br /><br>peripheral monocytes<br /><br>Cellular metabolism of hematopoetic stem cells and peripheral monocytes</p><br>