Effects of BCG vaccination on bone marrow cells.
Recruiting
- Conditions
- on specific effects of vaccines, ex-vivo cytokine responses, trained innate immunity. Niet specifieke effecten vaccinatie, ex-vivo cytokine responsen, getrainde aangeboren immuniteit
- Registration Number
- NL-OMON24361
- Lead Sponsor
- Radboudumc
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- Not specified
- Target Recruitment
- 20
Inclusion Criteria
Written informed consent
- Age ¡Ý18
Exclusion Criteria
- Use of systemic medication other than oral anti-contraceptive drugs
- Vaccination within 3 months prior to study period
Study & Design
- Study Type
- Interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method - Cellular composition of bone marrow compartment by FACS<br /><br>- Ex-vivo cytokine (IL-1â, TNF, IL-6, IL-10, IFNã) release of full bone marrow aspirate, PBMCs, CD34+ cell fraction and bone marrow derived macrophages upon stimulation with PAMPs (e.g. LPS) and pathogens (e.g. S. aureus, C. albicans, M. tuberculosis, S. pneumonia)<br /><br>Secondary study parameters<br>
- Secondary Outcome Measures
Name Time Method - Glycolytic activity of bone marrow, spleen and lymph nodes by use of FDG-PET by measuring standardized uptake values in predesignated volumes of interest.<br /><br>- Functional assessment of PBMC function <br /><br>- Hematopoietic lineage differentiation<br /><br>- Gene expression of HSPCs and peripheral blood monocytes<br /><br>- Epigenetic changes in HSPCs and peripheral blood monocytes<br /><br>- Cellular metabolism of HSPCs and peripheral blood monocytes<br /><br>- SNP analysis for SNPs known to influence innate immune function will be analysed and correlated with induction of trained immunity in PBMCs and HSPCs <br /><br>