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ChulaCov19 Vaccine in Healthy Adults

Phase 1
Completed
Conditions
COVID-19 Vaccine
Safety Issues
Interventions
Biological: ChulaCov19 vaccine
Other: Placebo
Registration Number
NCT04566276
Lead Sponsor
Chulalongkorn University
Brief Summary

This study will be conducted in 2 phases. Phase 1 of this study will be a single-centre, open label, dose escalation first in human (FIH) study conducted in 2 groups of healthy participants. Group 1 will enrol adults aged 18-55 years (inclusive); Group 2 will enroll elderly adults (elderly) aged 56-75 years (inclusive).

Phase 2 of this study will be a single centre, the proposed design will be observer-blind, placebo-controlled study to assess the safety, reactogenicity, and immunogenicity of ChulaCov19 vaccine in healthy adults (18-75 years of age inclusive).

Detailed Description

This study will be conducted as a combined phase 1/2 study in healthy participants.

The first phase of the study will evaluate the safety, tolerability, and reactogenicity of escalating doses (10 µg, 25 µg, and 50 µg) of the ChulaCov19 vaccine, administered intramuscularly (IM) according to a repeat vaccination schedule (given 21 days apart) in healthy adults aged 18-55 years and in elderly adults aged 56-75 years, up to Visit 10 (Day 50 ±3).

The second phase of the study will evaluate the safety, tolerability, and reactogenicity of escalating doses of the ChulaCov19 vaccine, administered intramuscularly (IM) according to a repeat vaccination schedule (given 21 days apart) in healthy adults aged 18--75 years, up to Visit 10 (Day 50 ±3). The study will also evaluate the immunogenicity measured as neutralising antibody titre (measured by Micro-viral neutralising test \[MicroVNT\]) following repeat vaccination of escalating doses of the ChulaCov19 vaccine, administered IM according to a repeat vaccination schedule (given 21 days apart) in healthy adults aged 18-75 years, at Visit 9 (Day 29 +3).

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
192
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
SEQUENTIAL
Arm && Interventions
GroupInterventionDescription
Elderly Cohort 3: 50 µgChulaCov19 vaccine12 elderlies aged 56-75 years will receive 50 µg of the vaccine IM
Adult Cohort 1: 10 µgChulaCov19 vaccine12 healthy adults aged 18-55 years will receive 10 µg of the vaccine IM
Adult Cohort 3: 50 µgChulaCov19 vaccine12 healthy adults aged 18-55 years will receive 50 µg of the vaccine IM
Elderly Cohort 2: 25 µgChulaCov19 vaccine12 elderlies aged 56-75 years will receive 25 µg of the vaccine IM
Adult Cohort 2: 25 µgChulaCov19 vaccine12 healthy adults aged 18-55 years will receive 25 µg of the vaccine IM
Elderly Cohort 1 :10 µgChulaCov19 vaccine12 elderlies aged 56-75 years will receive 10 µg of the vaccine IM
Phase 2: PlaceboPlaceboadults between 18 and 59 years of age will receive 2 IM saline vaccinations; administered 21days apart (on Day 1 and Day 22 ±3)
Phase 2: ChulaCov19 vaccine Dose 50 ugChulaCov19 vaccineadults between 18 and 59 years of age will receive 2 IM ChulaCov19 vaccine Dose 50 ug vaccinations; administered 21days apart (on Day 1 and Day 22 ±3)
Primary Outcome Measures
NameTimeMethod
Phase 1 and 2: Frequency of Adverse Eventsup to Day 50

Frequency of Adverse Events

Phase 1 and 2: Grade of Adverse Eventsup to Day 50

Grade of Adverse Events

Phase 1 and 2: Frequency of solicited reportable systemic reactogenicity Adverse Eventsduring a 7-day follow-up period post each vaccination

Frequency of solicited reportable systemic Adverse Events: (i.e., headache, fatigue, myalgia, malaise, fever, rigors, arthralgia, nausea/vomiting, diarrhea, light headedness, dizziness, or any other symptoms)

Phase 1 and 2: Changes in vital signsup to Day 50

Changes in vital signs: (i.e., body temperature, respiratory rate, pulse rate, systolic blood pressure (SBP), and diastolic blood pressure (DBP))

Phase 1 and 2: Changes in physical examinationsup to Day 50

Changes in physical examinations: (i.e., head, ears, nose, throat, lungs, lymph nodes, heart, abdomen and skin)

Phase 1 and 2: Frequency of solicited reportable local Adverse Eventsduring a 7-day follow-up period post each vaccination

Frequency of solicited reportable local Adverse Events (i.e., pain, tenderness, erythema/redness, induration/swelling, ulceration, scabs, ecchymosis, oedema, itching, paraesthesia, and hypersensitivity)

Phase 1 and 2: Frequency of Medically-Attended Adverse Eventsup to Day 387

Frequency of Medically-Attended Adverse Events

Phase 1 and 2: Frequency of New-Onset Chronic Medical Conditionsup to Day 387

Frequency of New-Onset Chronic Medical Conditions

Phase 1 and 2: Grade of solicited reportable local Adverse Eventsduring a 7-day follow-up period post each vaccination

Grade of solicited reportable local Adverse Events: (i.e., pain, tenderness, erythema/redness, induration/swelling, ulceration, scabs, ecchymosis, oedema, itching, paraesthesia, and hypersensitivity)

Phase 1 and 2: Presence of injection site reactionsup to Day 50

Presence of injection site reactions

Phase 2: Geometric mean titers (GMT) in SARS-CoV-2-specific serum neutralising antibody levelsat Day 29 (7 days after the second dose)

Geometric mean titers (GMT) in SARS-CoV-2-specific serum neutralising antibody levels

Phase 1 and 2: Frequency of Serious Adverse Eventsup to Day 387

Frequency of Serious Adverse Events

Phase 1 and 2: Changes in laboratory measurementsup to Day 50

Changes in laboratory measurements: (i.e., haemoglobin (Hb), haematocrit (HCT), white blood cells (WBC), neutrophil, lymphocytes, eosinophil, basophil, monocytes, platelet, sodium, potassium, chloride, bicarbonate, blood urea nitrogen (BUN), creatinine, total protein, albumin, lipase, phosphorus, gamma-glutamyl transferase (GGT), glucose, creatinine phosphokinase (CPK), calcium, uric acid, C-reactive protein (CRP), alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total bilirubin, estimated glomerular filtration rate (eGFR), prothrombin time (PR), partial thromboplastin time (PTT) and international normalized ratio (INR))

Phase 1 and 2: Grade of of solicited reportable systemic Adverse Eventsduring a 7-day follow-up period post each vaccination

Grade of solicited reportable systemic Adverse Events: (i.e., headache, fatigue, myalgia, malaise, fever, rigors, arthralgia, nausea/vomiting, diarrhea, light headedness, dizziness, or any other symptoms)

Secondary Outcome Measures
NameTimeMethod
Phase 1 and Phase 2: Proportion of participants who achieved a greater than or equal to 4-fold rise from before vaccination in SARS-CoV-2-specific serum neutralising antibody levelsAt Day 29

Proportion of participants who achieved a greater than or equal to 4-fold rise from before vaccination in SARS-CoV-2-specific serum neutralising antibody levels

Phase 1 and Phase 2: Percentage of participants who have positive specific CD4 T-cell IFNγ ELISpot responsesDay 29

Percentage of participants who have positive specific CD4 T-cell IFNγ ELISpot responses

Phase 1 and Phase 2: Percentage of participants who have positive specific CD8 T-cell IFNγ ELISpot responsesDay 29

Percentage of participants who have positive specific CD8 T-cell IFNγ ELISpot responses

Phase 1 and Phase 2: Geometric mean titers (GMT) of SARS-Cov2-spike protein-binding IgG antibodyat Day 29

Geometric mean titers (GMT) of SARS-Cov2-spike protein-binding IgG antibody

Phase 1 and Phase 2: Geometric mean fold rises (GMFR) in SARS-Cov2-spike protein-binding IgG antibodyfrom baseline to Day 29

Geometric mean fold rises (GMFR) in SARS-Cov2-spike protein-binding IgG antibody

Phase 1 and Phase 2: Geometric mean fold rises (GMFR) in SARS-CoV-2-specific serum neutralising titersfrom baseline to Day 29

Geometric mean fold rises (GMFR) in SARS-CoV-2-specific serum neutralising titers

Phase 1 and Phase 2: Proportion of participants who seroconverted: achieving a greater than or equal to 4-fold rise in SARS-Cov2-spike protein-binding IgG antibodyfrom baseline to Day 29

Proportion of participants who seroconverted: achieving a greater than or equal to 4-fold rise in SARS-Cov2-spike protein-binding IgG antibody

Phase 1 and Phase 2: Median number of spot-forming cells (SFC) per 1 million PBMCsDay 29

Median number of spot-forming cells (SFC) per 1 million PBMCs

Phase 1 and Phase 2: Percentage of participants who shows positive specific Th1 responsesDay 29

Percentage of participants who shows positive specific Th1 responses

Phase 1 and Phase 2: Median percentage specific Th2 responsesDay 29

Median percentage specific Th2 responses

Phase 1: Geometric mean titers (GMT) in SARS-CoV-2-specific serum neutralising antibody levelsat Day 29 (7 days after the second dose)

Geometric mean titers (GMT) in SARS-CoV-2-specific serum neutralising antibody levels

Phase 1 and Phase 2: Geometric mean titers (GMT) in SARS-CoV-2 surrogate viral neutralising antibody levelsat Day 29

Geometric mean titers (GMT) in SARS-CoV-2 surrogate viral neutralising antibody levels

Phase 1 and Phase 2: Proportion of participants who achieved a greater than or equal to 4-fold rise from before vaccination in SARS-CoV-2 surrogate viral neutralising antibody levelsat Day 29

Proportion of participants who achieved a greater than or equal to 4-fold rise from before vaccination in SARS-CoV-2 surrogate viral neutralising antibody levels

Phase 1 and Phase 2: Geometric mean fold rises (GMFR) in SARS-CoV-2 surrogate viral neutralising antibody titersfrom baseline to Day 29

Geometric mean fold rises (GMFR) in SARS-CoV-2 surrogate viral neutralising antibody titers

Phase 1 and Phase 2: Median percentage specific Th1 responsesDay 29

Median percentage specific Th1 responses

Phase 1 and Phase 2: Percentage of participants who shows positive specific Th2 responsesDay 29

Percentage of participants who shows positive specific Th2 responses

Trial Locations

Locations (2)

Chula Vaccine Research Center (ChulaCRC) Faculty of Medicine Chulalongkorn University

🇹🇭

Bangkok, Thailand

Center of Excellence for Vaccine Trial (Vaccine Trial Centre), Faculty of Tropical Medicine Mahidol University

🇹🇭

Bangkok, Thailand

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