Protocol to Obtain Bone Marrow for Research
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 28
- 试验地点
- 1
- 主要终点
- Regulation of human globin gene expression
研究概览
简要总结
Potential donors who are undergoing bone marrow biopsy or bone marrow donation as part of clinical care will be asked by the principal investigator (PI) if they might be interested in donating marrow for this research project. If they are interested, the PI will meet with the prospective donor to review the information contained in the informed consent document and to answer any questions the potential subject may have. Bone marrow aspirates of approximately 5cc will be obtained from the posterior iliac crest of subjects using standard techniques.
详细描述
The purpose of this study is to determine the mechanisms that regulate human globin gene expression by analyzing the function of cell signaling pathways and the expression of other related genes and their protein products in the bone marrow cells of people without blood diseases. This includes the effects of drugs and other in vitro manipulations of the cells once they are removed from donors. These assays will include, but are not limited to, determinations of DNA methylation status, histone acetylation profiles, DNAse sensitivity, transcription factor binding to DNA, levels of various proteins, messenger RNAs and miRNAs in the cells of the marrow samples. By understanding how this process occurs in people without blood diseases, we hope to to design future treatments for people with diseases such as sickle cell disease and B-thalassemia.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Other
入排标准
- 年龄范围
- 17 Years 至 —（Child, Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Patients undergoing diagnostic bone marrow biopsies at the Norris Cotton Cancer Center of DHMC.
- •Patients undergoing bone marrow harvesting for autologous bone marrow transplantation.
- •Normal donors undergoing bone marrow harvesting for autologous bone marrow transplantation.
- •Normal persons over the age of 17 who volunteer to undergo bone marrow aspiration.
排除标准
- •Normal volunteers with known allergies to lidocaine (or similar analgesics)or betadine.
结局指标
主要结局
Regulation of human globin gene expression
时间窗: Ongoing
The purpose of this study is to determine the mechanisms that regulate human globin gene expression by analyzing the function of cell signaling pathways and the expression of other related genes and their protein products in the bone marrow cells of people without blood diseases.
次要结局
未报告次要终点
研究者
Christopher H. Lowrey
Professor of Medicine and of Pharmacology and Toxicology
Dartmouth-Hitchcock Medical Center