Evaluation and correlation of salivary matrix metalloproteinase 8 levels before and after application of MI varnish 38 percent silver diamine fluoride and nano silver fluoride in children with early childhood caries an in vivo study

A total of 45 children  who  meets  inclusion  criteria will be selected and divided into three  experimental groups, Group 1 ( n =15), Group 2 ( n = 15), Group  3 ( n= 15) . All   subjects will be instructed to refrain from eating  and drinking for 1 hour  before the saliva  sample collection. Pre-intervention unstimulated saliva sample will be collected by spitting method. The patients will be asked to rinse the mouth with filter water  twice before the collection . After the oral rinse, children  will be asked  to  spit  the saliva  into sterile tubes for a period of 5 minutes . Then these tubes will be sealed immediately after sample collection and refigerated at - 20 degree celsius and stored in  laboratory until further analysis. Group 1 - MI varnish, Group 2- 38% SDF , Group 3 -NSF will be applied on selected carious teeth  with micro brush and left for 2 minutes . Post  application  instructions will be given. Patients will be recalled after 1 month and 3 month and post- interventional unstimulated salivary samples will be collected by the same procedure as mentioned above. All samples will be subjected to ELISA to evaluate and compare salivary MMP- 8 levels. Results obtained will be subjected to statistical data.