Role of Dietary Protein Intake on Whole Body Protein Balance in Elderly Adults
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Protein Overload
- Sponsor
- University of Arkansas
- Enrollment
- 8
- Primary Endpoint
- Changes in whole body anabolism
- Status
- Completed
- Last Updated
- 3 years ago
Overview
Brief Summary
The investigators examined the effects of dietary protein intake in a mixed meal at two levels of protein amount on whole body protein metabolisms in older adults.
Detailed Description
The investigators determined the total anabolic response to an amount of protein intake commonly eaten with dinner (70g) as compared with the amount required to maximally stimulate muscle protein synthesis (35g). Men and women, ages \> 60 years will be studied under two levels of protein intake in mixed meals in random order (35g or 70g protein in isocaloric mixed meals). For two days prior to each stable isotope tracer study, subjects' dietary intake of calories and protein will be stabilized by providing subjects their entire diets. On the third day, a 8-hr metabolic study will be conducted in the UAMS Reynolds Institute on Aging (IOA) to determine the total anabolic response to the particular amount of beef intake, which will be repeated with the other amount of protein in mixed meal after a minimum 7-day washout interval. The investigators measured whole body protein kinetics, muscle fractional synthetic rate, and plasma amino acid profile.
Investigators
Eligibility Criteria
Inclusion Criteria
- •Men and women, ages \> 60 years
Exclusion Criteria
- •History of diabetes
- •History of malignancy in the 6 months prior to enrollment
- •History of gastrointestinal bypass surgery (Lapband, etc)
- •History of a chronic inflammatory condition or disease (Lupus, HIV/AIDS, etc)
- •Pregnant females
- •Subjects who do not or will not eat animal proteins
- •Subjects who cannot refrain from consuming protein or amino acid supplements during their participation in this study
- •Subjects who report regular resistance exercise (more than once per week)
- •Hemoglobin less than 9.5 mg/dL at the screening visit
- •Platelets less than 150,000 at the screening visit
Outcomes
Primary Outcomes
Changes in whole body anabolism
Time Frame: Change before and after a mixed meal intake for approximately 8 hours
Whole body net protein balance in gram protein
Changes in skeletal muscle synthetic rate
Time Frame: Change before and after a mixed meal intake for approximately 8 hours
Skeletal muscle fractional synthetic rate in % synthesis