Fat-induced Release of GLP-1 in Humans

Not Applicable

Completed

• Conditions: Type 2 Diabetes
• Interventions: Dietary Supplement: Tributyrin; Dietary Supplement: C8-diet oil; Dietary Supplement: Olive oil; Dietary Supplement: Carrot

• Registration Number: NCT02264951
• Lead Sponsor: Glostrup University Hospital, Copenhagen

Brief Summary

This study compared the incretin-releasing effect of C8-diet oil and tributyrin with that of 20 ml olive oil on a molar basis in human volunteers.

Detailed Description

A structured triglyceride (1,3-di-octanoyl-2-oleoyl-glycerol, called C8-diet oil), which upon digestion results in generation of two medium-chain fatty acids and 2-oleoyl-glycerol (2-OG), may lead to activation of GPR119 only, since medium fatty acids are poor agonists for fatty acid receptors.

Study Timeline

• Study Start: 2013-10
• Primary Completion: 2013-12
• Study Completion: 2014-02
• Status Verified: 2014-10
• Study First Submitted: 2014-10-09
• Study First Submitted QC: 2014-10-09
• Last Update Submitted: 2014-10-09
• Study First Posted: 2014-10-15
• Last Update Posted: 2014-10-15

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 12

Inclusion Criteria

• Caucasian healthy male subjects
• Body mass index (BMI) 19 to 25 kg/m2
• Informed consent

Exclusion Criteria

• Anaemia
• Diabetes mellitus
• Gastrointestinal diseases or operations interacting with intestinal absorption

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
A meal containing carrot and tributyrin	Tributyrin	A meal containing 200 g grated carrot and 0.0216 mol tributyrin; blood test taken from 0 - 2 hours postprandial
A meal containing carrot and C8-diet oil	Carrot	A meal containing 200 g grated carrot and 0.0216 mol of C8-diet oil; blood test taken from 0 - 2 hours postprandial
A meal containing carrot	Carrot	A meal containing 200 g grated carrot; blood test taken from 0 - 2 hours postprandial
A meal containing carrot and tributyrin	Carrot	A meal containing 200 g grated carrot and 0.0216 mol tributyrin; blood test taken from 0 - 2 hours postprandial
A meal containing carrot and C8-diet oil	C8-diet oil	A meal containing 200 g grated carrot and 0.0216 mol of C8-diet oil; blood test taken from 0 - 2 hours postprandial
A meal containing carrot and olive oil	Olive oil	A meal containing 200 g grated carrot and 0.0216 mol of olive oil; blood test taken from 0 - 2 hours postprandial
A meal containing carrot and olive oil	Carrot	A meal containing 200 g grated carrot and 0.0216 mol of olive oil; blood test taken from 0 - 2 hours postprandial

Primary Outcome Measures

Name	Time	Method
plasma GLP-1	2 hours postprandial
plasma GIP	2 hours postprandial

Secondary Outcome Measures

Name	Time	Method
plasma insulin	2 hours postprandial
plasma PYY	2 hours postprandial
plasma glucose	2 hours postprandial
plasma neurotensin	2 hour postprandial
plasma CCK	2 hour postprandial