MedPath

Tocilizumab in Lung Transplantation

Phase 2
Recruiting
Conditions
Lung Transplant
Interventions
Registration Number
NCT06033196
Lead Sponsor
National Institute of Allergy and Infectious Diseases (NIAID)
Brief Summary

This is a trial in which 350 primary lung transplant recipients will be randomized (1:1) to receive either Tocilizumab (six doses over 20 weeks) plus standard triple maintenance immunosuppression or placebo (sterile normal saline) plus standard triple maintenance immunosuppression (Tacrolimus, Mycophenolate Mofetil, corticosteroids).

The primary objective is to test the hypothesis that treatment with triple maintenance immunosuppression plus Tocilizumab (TCZ) is superior to triple maintenance immunosuppression plus placebo (saline) as defined by a composite endpoint of a) CLAD, b) listed for re-transplantation, and c) death

Detailed Description

Not available

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
350
Inclusion Criteria

Study Entry:

  1. Subject and/or parent guardian must be able to understand the purpose of the study and willing to participate and sign informed consent/assent

  2. Greater than or equal to 30 kg body weight

  3. Listed for a primary lung transplant

  4. No previous or planned desensitization therapy prior to transplant

  5. Serum Immunoglobulin G (IgG) level greater than 400 mg/dL. Patients treated with intravenous immune globulin (IVIG) for hypogammaglobulinemia are eligible for enrollment if their serum IgG level is greater than 400 mg/dL 14 or more days after the most recent IVIG treatment

  6. For women of child-bearing potential, willingness to use highly-effective contraception; according to the Food and Drug Administration (FDA) Office of Women's Health (http://www.fda.gov/birthcontrol).

    Female participants of child-bearing potential must consult with their physician and determine the most suitable method(s) from this list to be used for the duration of the study. Those who choose oral contraception must agree to use a second form of contraception after administration of study drug for a period of 1 year after the last dose of study drug

  7. Tested negative for latent TB infection (LTBI) using a PPD or interferon-gamma release assay (i.e., QuantiFERON-TB, T-SPOT.TB) within 1 year prior to transplant or has completed appropriate LTBI therapy within the 1 year prior to transplant

  8. In the absence of contraindication, vaccinations must be up to date per the Division of Allergy, Immunology, and Transplantation (DAIT) Guidance for Patients in Transplant Trials

Randomization:

  1. Provide written informed consent for the study participation, and agree to continue in the study
  2. Undergoing single or bilateral lung transplant
  3. Agreement to use contraception; according to the FDA Office of Women's Health (http://www.fda.gov/birthcontrol), there are a number of birth control methods that are more than 80% effective. Female participants of child-bearing potential must consult with their physician and determine the most suitable method(s) from this list to be used for the duration of the study. Those who choose oral contraception must agree to use a second form of contraception after administration of study drug for a period of 1 year after the last dose of study drug
  4. Negative physical crossmatch, if the results are available at the time of randomization
  5. No desensitization therapy prior to transplant
  6. Negative pregnancy test (serum or urine) for women of child-bearing potential within 48 hours prior to transplant
  7. Negative severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) Polymerase Chain Reaction (PCR) testing for the recipient prior to transplant as per institutional policy
  8. Negative SARS-CoV2 PCR testing for the donor prior to transplant as per institutional policy
  9. Recipient of lungs that have been supported with ex vivo lung perfusion (EVLP) devices are permitted
Exclusion Criteria

Study Entry:

  1. Listed for multi-organ transplant (e.g., heart-lung, liver-lung, kidney-lung)
  2. Prior history of allogeneic organ or cellular transplantation
  3. Received treatment to deplete Human Leukocyte Antigens (HLA) antibodies before transplantation
  4. Currently breast-feeding a child or plans to become pregnant during the timeframe of the study follow up period
  5. History of severe allergic and/or anaphylactic reactions to humanized or murine monoclonal antibodies
  6. Known hypersensitivity or previous treatment with ACTEMRA(R) (tocilizumab) within the last 3 months
  7. Infection with human immunodeficiency virus (HIV)
  8. Hepatitis B virus surface antigen or core antibody positive
  9. Hepatitis C virus PCR positive (HCV+) patients who have failed to demonstrate sustained viral remission (2 consecutive PCR or Nucleic Acid Tests (NAT) negative tests at least 24 weeks apart), with or without anti-viral treatment;
  10. Chronic infection with Burkholderia cenocepacia or Burkholderia gladioli
  11. Non-tuberculous mycobacterial (NTM) pulmonary disease; if there is a history of NTM pulmonary disease, culture conversion is necessary for eligibility
  12. Presence of active malignancy or history of malignancy less than 5 years in remission, excluding adequately treated in-situ cervical carcinoma, low grade prostate carcinoma, or adequately treated basal or squamous cell carcinoma of the skin
  13. History of hemolytic-uremic syndrome/ thrombotic thrombocytopenia purpura
  14. History of demyelinating disorders (e.g., multiple sclerosis, chronic inflammation demyelinating polyneuropathy)
  15. Current treatment with alkylating agents such as cyclophosphamide
  16. History of gastrointestinal (GI) tract perforation
  17. History of inflammatory bowel disease except fully excised ulcerative colitis
  18. Any history of diverticulitis (event if not perforated) or confirmed diverticular bleeding. (Diverticulosis is not an exclusion).
  19. Patients with a platelet count < 100,000/mm^3 (last measurement within 7 days prior to enrollment)
  20. Patients with an absolute neutrophil count (ANC) < 2,000/mm^3 (last measurement within 7 days prior to enrollment)
  21. Patients with Aspartate Aminotransferase (AST) or Alanine Aminotransferase (ALT) levels >3 times upper limit of normal
  22. Patients who use illegal drugs
  23. Use of investigational drugs within 4 weeks prior to enrollment
  24. Any condition that in the opinion of the site Principal Investigator (PI) introduces undue risk by participating in this study

Randomization:

  1. Recipient of multi-organ or tissue transplants
  2. Received a live virus vaccine within 30 days prior to randomization
  3. Received treatment to deplete HLA antibodies before transplantation to improve the possibility of transplantation
  4. Patients with known donor-specific antibody that will require intervention based on local clinical protocols
  5. History of GI tract perforation
  6. History of inflammatory bowel disease except fully excised ulcerative colitis
  7. History of diverticulitis (diverticulosis is not an exclusion) or diverticular bleeding
  8. History of severe allergic anaphylactic reactions to humanized or murine monoclonal antibodies
  9. Previous treatment with ACTEMRA(R) (tocilizumab) within the last 3 months.
  10. Recipient or donor with infection with human immunodeficiency virus (HIV)
  11. Recipient with hepatitis B virus surface antigen or hepatitis B core antibody positive
  12. Hepatitis B negative transplant recipient that received a transplant from a Hepatitis B core antibody positive donor unless the recipient has a Hepatitis B Surface Antigen (HBsAb) titer >10U/L
  13. Recipient of a hepatitis C virus nucleic acid test (NAT) positive donor organ
  14. Latent TB infection (LTBI) and has not completed appropriate therapy
  15. Chronic infection with Burkholderia cenocepacia or Burkholderia gladioli
  16. Non-tuberculous mycobacterial (NTM) pulmonary disease; if there is a history of NTM pulmonary disease, culture conversion is necessary for eligibility
  17. Presence of active malignancy (except for non-melanoma skin cancer)
  18. History of hemolytic-uremic syndrome/ thrombotic thrombocytopenia purpura
  19. History of demyelinating disorders (e.g., multiple sclerosis, chronic inflammation demyelinating polyneuropathy)
  20. Current treatment with alkylating agents such as cyclophosphamide
  21. Patients with AST or ALT levels > 1.5 times upper limit of normal (last measurement within 1 day prior to randomization)
  22. Patients with platelet count <100,000/mm^3 (last measurement within 1 day prior to randomization)
  23. Patients with an absolute neutrophil count (ANC) <2,000/mm^3 (last measurement within 1 day prior to randomization)
  24. Patients who are administered or intended to be administered cytolytic (such as anti-thymocyte globulin) or anti-CD25 monoclonal antibody agents as induction therapy in the immediate post- transplant period
  25. Patients who have been treated in the past 3 months, or for whom it is anticipated that treatment with any immunomodulatory biological agents post-transplant are excluded
  26. Use of an investigational drug after transplant
  27. Any condition that in the opinion of the site PI introduces undue risk by participating in this study

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Placebo GroupPlacebo for TocilizumabSubject in this group will receive placebo for Tocilizumab (sterile normal saline) plus standard triple maintenance immunosuppression of Tacrolimus, Mycophenolate Mofetil, corticosteroids
Tocilizumab GroupTocilizumabSubject in this group will receive ACTEMRA(R) (Tocilizumab) ,(six injections over 20 weeks) plus standard triple maintenance immunosuppression of Tacrolimus, Mycophenolate Mofetil, corticosteroids
Primary Outcome Measures
NameTimeMethod
Proportion of subjects who meet any one of the pre-specified events detailed in the outcome description: from Baseline up to 36 monthsOver a period of 3 years after transplantation

1. The development of Chronic Lung Allograft Dysfunction (CLAD)

* The development of any form of CLAD will be defined according to the standard 2019 The International Society for Heart and Lung Transplantation (ISHLT) criteria.

2. Listed for re-transplantation

* Re-transplantation defined as the subject has been formally registered on the United Network for Organ Sharing (UNOS) waiting list to undergo a second lung transplant surgery

3. Death

* Primary analysis will be conducted according to an Intent-to-treat (ITT) principle and therefore will include all randomized subjects who receive Tocilizumab(TCZ) or placebo. The time from randomization to development of CLAD will be compared between the two treatment groups (TCZ vs. placebo) using a Pearson's chi-square test.

Secondary Outcome Measures
NameTimeMethod
Time to the onset of Chronic Lung Allograft Dysfunction (CLAD)At 3 years after transplantation
Time to the onset of CLAD, being listed for re-transplantation, or deathAt 3 years after transplantation
Cumulative incidence of Chronic Lung Allograft Dysfunction (CLAD)At 3 years after transplantation
Cumulative incidence listed for re-transplantationAt 3 years after transplantation
Cumulative incidence of deathAt 3 years after transplantation
Incidence of Primary Graft Dysfunction (PGD) grade 3At any timepoint in the first 72 hours
Freedom from Acute Cellular Rejection (ACR) grade >=A2At 3 years after transplantation

Transbronchial lung biopsies will be performed according to the local center standard of care using the 2007 International Society for Heart and Lung Transplantation (ISHLT) criteria.

Acute Cellular Rejection (A grade Rejection) Scale:

* None (A0)

* Minimal (A1)

* Mild (A2)

* Moderate (A3)

* Severe (A4)

* Ungradable (AX)

Proportion of subjects free from Antibody Mediated Rejection (AMR)At 3 years after transplantation

Antibody mediated rejection studies will be performed using original H\&E stained slides, trichrome/elastic trichrome stain, and C4d immunostain (5 slides per case), along with positive controls

Proportion of subjects free from the development of de novo donor specific antibodies (dnDSA)At 3 years after transplantation
Incidence of Gastrointestinal (GI) tract perforationAt 3 years after transplantation
Incidence of serious infections requiring intravenous antimicrobial therapy and need for hospitalizationAt 3 years after transplantation
Incidence of confirmed bacterial infection requiring antimicrobial therapyAt 3 years after transplantation
Incidence of confirmed Cytomegalovirus (CMV) infection requiring antimicrobial therapyAt 3 years after transplantation
Incidence of confirmed mold infection requiring antimicrobial therapyAt 3 years after transplantation
Incidence of confirmed mycobacterial infection requiring antimicrobial therapyAt 3 years after transplantation
Incidence of confirmed community-acquired respiratory viral infection, including coronavirus disease 2019 (COVID-19) infectionAt 3 years after transplantation
Incidence of discontinuation of Tocilizumab (TCZ) due to an adverse eventAt 3 years after transplantation
Incidence of discontinuation of Tocilizumab (TCZ) due to serious adverse eventAt 3 years after transplantation
Incidence of discontinuation of Tocilizumab (TCZ) placebo due to an adverse eventAt 3 years after transplantation
Incidence of discontinuation of Tocilizumab (TCZ) placebo due to serious adverse eventAt 3 years after transplantation
Incidence of malignancy excluding squamous or basal cell skin cancerAt 3 years after transplantation
Incidence of Tuberculosis (TB)At 3 years after transplantation
Incidence of Post-transplant lymphoproliferative disorder (PTLD)At 3 years after transplantation
Time to the onset of being listed for re-transplantationAt 3 years after transplantation
Time to the onset of deathAt 3 years after transplantation

Trial Locations

Locations (15)

St. Joseph's Hospital and Medical Center (Site #: 71192)

🇺🇸

Phoenix, Arizona, United States

Cedars Sinai Medical Center (Site #: 71146)

🇺🇸

Los Angeles, California, United States

David Geffen School of Medicine at UCLA (Site #: 71123)

🇺🇸

Los Angeles, California, United States

University of Maryland Medical Center (Site #: 71138)

🇺🇸

Baltimore, Maryland, United States

Massachusetts General Hospital (Site #: 71107)

🇺🇸

Boston, Massachusetts, United States

Boston Children's Hospital (Site #: 71001)

🇺🇸

Boston, Massachusetts, United States

Barnes Jewish Hospital/ Washington University SOM (Site #: 71191)

🇺🇸

Saint Louis, Missouri, United States

St. Louis Children's Hospital of Washington University (Site #: 71006)

🇺🇸

Saint Louis, Missouri, United States

Columbia University Medical Center (Site #: 71113)

🇺🇸

New York, New York, United States

Duke University Medical Center (Site #: 71139)

🇺🇸

Durham, North Carolina, United States

Houston Methodist Hospital (Site #: 71120)

🇺🇸

Houston, Texas, United States

University of Utah Medical Center (Site #: 71126)

🇺🇸

Salt Lake City, Utah, United States

Cleveland Clinic Lerner College Medicine (Site #: 71101)

🇺🇸

Cleveland, Ohio, United States

University of Texas Southwestern (Site #: 71187)

🇺🇸

Dallas, Texas, United States

University Health Network/ Toronto General Hospital (Site #: 71121)

🇨🇦

Toronto, Ontario, Canada

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