A Comparison Between Home-made Vitrification & Thawing Media and a Commercially Available Brand on Blastocyst Survival, Expansion and Implantation
试验速览
- 阶段
- 2 期
- 状态
- 已完成
- 入组人数
- 74
- 主要终点
- blastocyst immediate survival
研究概览
简要总结
This study was conducted to evaluate and compare cryo-survival, re-expansion, and implantation rates of Day 5/6 human blastocysts that were vitrified with home-made media (HM) according to (Shady grove protocol) and warmed with gradual Thawing HM according to (Kuwayama protocol)with Global Fast Freeze/Thaw Kits (LifeGlobal, Canada).
详细描述
Group 1 blastocysts were vitrified with the Vit HM according to shady grove protocol using open system on cryotopvit. device and thawed with gradual thawing technique using HM according to Kuwayama protocol. Group 2 blastocysts were cryopreserved with the Global Fast Freeze Kitusing open system on cryotopvit. device and thawed using Gobal fast thaw kit. Blastocysts were subsequently cultured for 6-8 h, assessed for survival and expansion, then transferred and the percentages of Positive hCG, clinical pregnancy, implantation, multiple pregnancy and abortion were recorded.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Basic Science
- 盲法
- Double (Care Provider, Investigator)
盲法说明
only the investigator knew the procedure and the media provided for each case
入排标准
- 年龄范围
- 22 Years 至 37 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •age of the patients 22-37
- •All vitrified & thawed blastocyst.
- •unexplained cases or ovarian factors for infertility.
排除标准
- •extremes of age
- •cases of uterine factor
- •endometriosis
研究组 & 干预措施
Home made media
home made vitrification & thawing kit
干预措施: home made vitrification & thawing kit (Biological)
Commercial media
commercially available vitrification & thawing kit
干预措施: commercially made vitrification & thawing kit (Biological)
结局指标
主要结局
blastocyst immediate survival
时间窗: 0 hour at time of thawing
The effect of Vitrification protocol on the blastocyst immediate survival
次要结局
- live birth rate(9 months)
- Blastocyst survival at 6 hours(6 hours after thawing)
- pregnancy rate(2 weeks after transfer)
研究者
Ahmed Saad
Assistant professor of Ob. & Gyn.
Benha University