Effect of Polycystic ovarian syndrome(PCOS) on female egg and its surrounding environment

Not yet recruiting

• Conditions: Abnormal findings in other body fluids and substances,

• Registration Number: CTRI/2020/02/023320
• Lead Sponsor: Department of Clinical Embryology

Brief Summary

Polycysticovary syndrome (PCOS) is a common metabolic dysfunction and heterogeneousendocrine disorder in women of reproductive age. In PCOS, due to ovarianhyperandrogenism and hyperinsulinemia from insulin resistance and paracrinedysregulation of growth factors, including transforming growth factor- β(TGFβ)-related proteins disrupt the intrafollicular environment, altergranulosa cell oocyte interactions and impair cytoplasmic and/or nuclearmaturation of the oocyte. However, the exact pathophysiology is remaining to beelucidated.  Recent studieshave shown that there is significant changes in follicular microenvironment (Leoet al., 2016), altered gene expression profiles (Haouzi et al., 2012) and methylation patternin granulosa cells (Sagvekar et al., 2019) derived from PCOS patients. However,none of the studies have examined the expression of de novo methyl transferases(DNMT’s) which are responsible for additionof methyl group to CpG island of promoter regions thereby altering themethylation status and regulate the gene expression of the granulosa cells. Understanding the DNMT’s expression and global methylation patternin granulosa cells from PCOS patients and its association with altered oocytestructure will be of significant value in unravel the impact of PCOS on oocyteand embryo quality. Hence, the current study will examine the expression ofDNMTs and global methylation of granulosa cells and ultra-structuralmodification of oocytes in women with PCOS.

Detailed Description

Not available

Study Timeline

• Study Start: 2020-02-19
• Last Update Posted: 2020-10-02

Recruitment & Eligibility

• Status: Not Yet Recruiting
• Sex: Female
• Target Recruitment: 60

Inclusion Criteria

Subjects with and without PCOS, age < 35 years were included.

Exclusion Criteria

Patients with endometriosis, diabetics, hyperthyroidism or age > 35 years were excluded from the study.

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
From this study we are expected to uderstand the level of testosterone and luteinizing hormone in follicular fluid, gene expression of de novo methyl transferase in granulosa cells and global methylation, ultrastructure of immature oocyte and fertilization and embryonic development	10.09.2019 to 09.09.2020

Secondary Outcome Measures

Name	Time	Method
Better understanding of PCOS characteristics	10.09.2019 to 09.09.2020

Trial Locations

Locations (1)

Kasturba Medical College; 🇮🇳 Udupi, KARNATAKA, India

Kasturba Medical College

🇮🇳Udupi, KARNATAKA, India

Anjali S

Principal investigator

9746195944

anjali.s4@learner.manipal.edu