The effects of acute exercise on (myokine) gene expression in human skeletal muscle

Completed

• Conditions: Type 2 Diabetes mellitus; diabetes; 10018424

• Registration Number: NL-OMON34451
• Lead Sponsor: Wageningen Universiteit

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Last Update Posted: 6 May 2024

Recruitment & Eligibility

• Status: Completed
• Sex: Not specified
• Target Recruitment: 10

Inclusion Criteria

• Age 40-60 years
• Male gender
• BMI < 30 kg/m2

Exclusion Criteria

• Exercising regularly (> 2 times a week, > 3 hour in total per week)
• Unstable body weight (weight gain or loss > 3 kg in the past three months)
• Following, or have recently followed a (weight-loss) diet
• Donated or intended to donate blood 2 months before until two months after the study
• Medical condition that can interfere with the study outcome (i.e. cardiovascular disease, pulmonary disease)
• Systolic blood pressure >160 mmHg and/or diastolic blood pressure >100 mmHg
• Use of medications known to interfere with gene expression in the muscles (i.e. statins, fenofibrate)
• Use of antithrombotic therapy (marcoumar, sintromitis).
• Diagnosed diabetes mellitus type 1 or 2.
• Drugs or alcohol abuse ( > 3 glasses of alcoholic beverages a day).
• (Chronic) injuries of the locomotor system that can interfere with the intervention
• Participated in another study within the last six months

Study & Design

• Study Type: Interventional
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
<p>Main study outcomes include upregulation of genes in skeletal muscle after<br /><br>exercise (with a focus on genes encoding myokines) and changes of blood plasma<br /><br>levels of selected proteins after exercise. The proteins are selected based on<br /><br>gene expression changes measured.</p><br>

Secondary Outcome Measures

Name	Time	Method
<p>PBMC gene expression: gene expression changes in the blood will be assessed<br /><br>Routine plasma levels: plasma levels of glucose, insulin, fatty acids,<br /><br>cortisol, adrenalin and lactate will be determined.<br /><br>Heart rate: heart rate will be measured.</p><br>