Influence of acute exercise on activation of the aryl hydrocarbon receptor (AhR) in CD4+ T cells in healthy adults
Not Applicable
Recruiting
- Conditions
- Physiological study with healthy participants. No health issue is being researched.
- Registration Number
- DRKS00028792
- Lead Sponsor
- TU Dortmund, Institut für Sport und Sportwissenschaft, Arbeitsbereich für Leistung und Gesundheit (Sportmedizin)
- Brief Summary
Not available
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- Recruiting
- Sex
- All
- Target Recruitment
- 28
Inclusion Criteria
Age: 20-40 years
German speaking (fluent)
BMI < 30
Exclusion Criteria
- chronic medical condition
- chronic intake of medication at study inclusion or in the past 6 months
- pregnancy or breast feeding
- oral contraceptives
- drug use in the past 6 months
- acute infection or orthopaedic injury
- use of supplements
- smoking
- > 300 minutes endurance exercise per week (during the past 6 months)
- > 3 sessions resistance exercise per week (during the past 6 months)
Study & Design
- Study Type
- interventional
- Study Design
- Not specified
- Primary Outcome Measures
Name Time Method AHR activation in CD4+ T cells (collected from venous blood) immediately after exercise compared to control. The primary endpoint is the comparison between intervention and control pre versus immediately post intervention / control cessation. AHR activation will be measured using art-PCR and flow cytometry.
- Secondary Outcome Measures
Name Time Method AHR activation at all other measurement time points ((1h, 3h, 24h and 48h after cessation of intervention/control) measured in CD4+ cells from venous blood. AHR activation will be assessed using qrt-PCR and flow cytometry.<br><br>- Tryptophan metabolite levels in blood serum and urine using HPLC MS/MS<br>- Inflammation markers ((anti-)inflammatory cytokine profile) using ELISA<br><br><br>